Function and Interaction of the Coupled Genes Responsible for Pik-h Encoded Rice Blast Resistance

Pik-h, an allele of Pik, confers resistance against the rice blast pathogen Magnaporthe oryzae. Its positional cloning has shown that it comprises a pair of NBS-LRR genes, Pikh-1 and Pikh-2. While Pikh-1 appears to be constitutively transcribed, the transcript abundance of Pikh-2 responds to pathoge...

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Autores principales: Zhai, Chun, Zhang, Yu, Yao, Nan, Lin, Fei, Liu, Zhe, Dong, Zhongqiu, Wang, Ling, Pan, Qinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045721/
https://www.ncbi.nlm.nih.gov/pubmed/24896089
http://dx.doi.org/10.1371/journal.pone.0098067
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author Zhai, Chun
Zhang, Yu
Yao, Nan
Lin, Fei
Liu, Zhe
Dong, Zhongqiu
Wang, Ling
Pan, Qinghua
author_facet Zhai, Chun
Zhang, Yu
Yao, Nan
Lin, Fei
Liu, Zhe
Dong, Zhongqiu
Wang, Ling
Pan, Qinghua
author_sort Zhai, Chun
collection PubMed
description Pik-h, an allele of Pik, confers resistance against the rice blast pathogen Magnaporthe oryzae. Its positional cloning has shown that it comprises a pair of NBS-LRR genes, Pikh-1 and Pikh-2. While Pikh-1 appears to be constitutively transcribed, the transcript abundance of Pikh-2 responds to pathogen challenge. The Pikh-1 CC (coiled coil) domain interacts directly with both AvrPik-h and Pikh-2. Transient expression assays demonstrated that Pikh-2 mediates the initiation of the host defence response. Nucleocytoplasmic partitioning of both Pikh-1 and Pikh-2 is required for their functionalities. In a proposed mechanistic model of Pik-h resistance, it is suggested that Pikh-1 acts as an adaptor between AvrPik-h and Pikh-2, while Pikh-2 transduces the signal to trigger Pik-h-specific resistance.
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spelling pubmed-40457212014-06-09 Function and Interaction of the Coupled Genes Responsible for Pik-h Encoded Rice Blast Resistance Zhai, Chun Zhang, Yu Yao, Nan Lin, Fei Liu, Zhe Dong, Zhongqiu Wang, Ling Pan, Qinghua PLoS One Research Article Pik-h, an allele of Pik, confers resistance against the rice blast pathogen Magnaporthe oryzae. Its positional cloning has shown that it comprises a pair of NBS-LRR genes, Pikh-1 and Pikh-2. While Pikh-1 appears to be constitutively transcribed, the transcript abundance of Pikh-2 responds to pathogen challenge. The Pikh-1 CC (coiled coil) domain interacts directly with both AvrPik-h and Pikh-2. Transient expression assays demonstrated that Pikh-2 mediates the initiation of the host defence response. Nucleocytoplasmic partitioning of both Pikh-1 and Pikh-2 is required for their functionalities. In a proposed mechanistic model of Pik-h resistance, it is suggested that Pikh-1 acts as an adaptor between AvrPik-h and Pikh-2, while Pikh-2 transduces the signal to trigger Pik-h-specific resistance. Public Library of Science 2014-06-04 /pmc/articles/PMC4045721/ /pubmed/24896089 http://dx.doi.org/10.1371/journal.pone.0098067 Text en © 2014 Zhai et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhai, Chun
Zhang, Yu
Yao, Nan
Lin, Fei
Liu, Zhe
Dong, Zhongqiu
Wang, Ling
Pan, Qinghua
Function and Interaction of the Coupled Genes Responsible for Pik-h Encoded Rice Blast Resistance
title Function and Interaction of the Coupled Genes Responsible for Pik-h Encoded Rice Blast Resistance
title_full Function and Interaction of the Coupled Genes Responsible for Pik-h Encoded Rice Blast Resistance
title_fullStr Function and Interaction of the Coupled Genes Responsible for Pik-h Encoded Rice Blast Resistance
title_full_unstemmed Function and Interaction of the Coupled Genes Responsible for Pik-h Encoded Rice Blast Resistance
title_short Function and Interaction of the Coupled Genes Responsible for Pik-h Encoded Rice Blast Resistance
title_sort function and interaction of the coupled genes responsible for pik-h encoded rice blast resistance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045721/
https://www.ncbi.nlm.nih.gov/pubmed/24896089
http://dx.doi.org/10.1371/journal.pone.0098067
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