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Separating NADH and NADPH fluorescence in live cells and tissues using FLIM
NAD is a key determinant of cellular energy metabolism. In contrast, its phosphorylated form, NADP, plays a central role in biosynthetic pathways and antioxidant defence. The reduced forms of both pyridine nucleotides are fluorescent in living cells but they cannot be distinguished, as they are spec...
Autores principales: | Blacker, Thomas S., Mann, Zoe F., Gale, Jonathan E., Ziegler, Mathias, Bain, Angus J., Szabadkai, Gyorgy, Duchen, Michael R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046109/ https://www.ncbi.nlm.nih.gov/pubmed/24874098 http://dx.doi.org/10.1038/ncomms4936 |
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