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Bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes

OBJECTIVE(S): This study investigated the effects of melatonin, famotidine, mirtazapine, and thiamine pyrophosphate on ischemia/reperfusion (I/R) injury in diabetic rats and evaluated oxidant and antioxidant marker measurement results. It also examined the effects of the drugs aimed at preventing in...

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Autores principales: Yapca, Omer Erkan, Turan, Mehmet Ibrahim, Borekci, Bunyamin, Akcay, Fatih, Suleyman, Halis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046235/
https://www.ncbi.nlm.nih.gov/pubmed/24904723
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author Yapca, Omer Erkan
Turan, Mehmet Ibrahim
Borekci, Bunyamin
Akcay, Fatih
Suleyman, Halis
author_facet Yapca, Omer Erkan
Turan, Mehmet Ibrahim
Borekci, Bunyamin
Akcay, Fatih
Suleyman, Halis
author_sort Yapca, Omer Erkan
collection PubMed
description OBJECTIVE(S): This study investigated the effects of melatonin, famotidine, mirtazapine, and thiamine pyrophosphate on ischemia/reperfusion (I/R) injury in diabetic rats and evaluated oxidant and antioxidant marker measurement results. It also examined the effects of the drugs aimed at preventing infertility that may result from I/R injury. MATERIALS AND METHODS: Diabetic rats were divided into a control group (IRC) to be exposed to I/R, an ovarian I/R + 2.2 mg/kg melatonin (IRML) group, an ovarian I/R + famotidine (IRFA) group, an ovarian I/R + 20 mg/kg mirtazapine (IRMR) group, an ovarian I/R + 20 mg/kg thiamine pyrophosphate (IRTP) group, and a sham operation (SO) group. RESULTS: In the control group exposed to I/R, the levels of the oxidant parameters Malondialdehyde (MDA) and Myeloperoxidase (MPO) were significantly higher compared with the SO group, while the levels of the antioxidant parameters glutathione (GSH), Glutathione peroxidase (GPO), Glutathione reductase (GSHRd), Glutathione S - transferase (GST), and Superoxide dismutase (SOD) were significantly lower. Melatonin, famotidine, mirtazapine, and thiamin pyrophosphate prevented a rise in oxidant parameters and a decrease in antioxidants in ovarian tissue exposed to I/R. However, apart from thiamin pyrophosphate, none of the drugs were able to prevent infertility caused by I/R injury. CONCLUSION: Prevention of ovarian I/R injury-related infertility in rats with induced diabetes is not through antioxidant activity. Thiamine pyrophosphate prevents infertility through an as yet unknown mechanism. This study suggests that thiamine pyrophosphate may be useful in the prevention of I/R-related infertility in diabetics.
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spelling pubmed-40462352014-06-05 Bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes Yapca, Omer Erkan Turan, Mehmet Ibrahim Borekci, Bunyamin Akcay, Fatih Suleyman, Halis Iran J Basic Med Sci Original Article OBJECTIVE(S): This study investigated the effects of melatonin, famotidine, mirtazapine, and thiamine pyrophosphate on ischemia/reperfusion (I/R) injury in diabetic rats and evaluated oxidant and antioxidant marker measurement results. It also examined the effects of the drugs aimed at preventing infertility that may result from I/R injury. MATERIALS AND METHODS: Diabetic rats were divided into a control group (IRC) to be exposed to I/R, an ovarian I/R + 2.2 mg/kg melatonin (IRML) group, an ovarian I/R + famotidine (IRFA) group, an ovarian I/R + 20 mg/kg mirtazapine (IRMR) group, an ovarian I/R + 20 mg/kg thiamine pyrophosphate (IRTP) group, and a sham operation (SO) group. RESULTS: In the control group exposed to I/R, the levels of the oxidant parameters Malondialdehyde (MDA) and Myeloperoxidase (MPO) were significantly higher compared with the SO group, while the levels of the antioxidant parameters glutathione (GSH), Glutathione peroxidase (GPO), Glutathione reductase (GSHRd), Glutathione S - transferase (GST), and Superoxide dismutase (SOD) were significantly lower. Melatonin, famotidine, mirtazapine, and thiamin pyrophosphate prevented a rise in oxidant parameters and a decrease in antioxidants in ovarian tissue exposed to I/R. However, apart from thiamin pyrophosphate, none of the drugs were able to prevent infertility caused by I/R injury. CONCLUSION: Prevention of ovarian I/R injury-related infertility in rats with induced diabetes is not through antioxidant activity. Thiamine pyrophosphate prevents infertility through an as yet unknown mechanism. This study suggests that thiamine pyrophosphate may be useful in the prevention of I/R-related infertility in diabetics. Mashhad University of Medical Sciences 2014-04 /pmc/articles/PMC4046235/ /pubmed/24904723 Text en Copyright: © Journal Management System. Created by sinaweb http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yapca, Omer Erkan
Turan, Mehmet Ibrahim
Borekci, Bunyamin
Akcay, Fatih
Suleyman, Halis
Bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes
title Bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes
title_full Bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes
title_fullStr Bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes
title_full_unstemmed Bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes
title_short Bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes
title_sort bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046235/
https://www.ncbi.nlm.nih.gov/pubmed/24904723
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