Cargando…

Immunohistological and electrophysiological characterization of Globose basal stem cells

OBJECTIVE(S): In the past few decades, variety of foetal, embryonic and adult stem and progenitor cells have been tried with conflicting outcome for cell therapy of central nervous system injury and diseases. Cellular characteristics and functional plasticity of Globose basal stem cells (GBCs) resid...

Descripción completa

Detalles Bibliográficos
Autores principales: Thakur, Avinash, Muniswami, Duraimurugan, Tharion, George, Kanakasabapathy, Indirani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046242/
https://www.ncbi.nlm.nih.gov/pubmed/24904721
_version_ 1782480259373858816
author Thakur, Avinash
Muniswami, Duraimurugan
Tharion, George
Kanakasabapathy, Indirani
author_facet Thakur, Avinash
Muniswami, Duraimurugan
Tharion, George
Kanakasabapathy, Indirani
author_sort Thakur, Avinash
collection PubMed
description OBJECTIVE(S): In the past few decades, variety of foetal, embryonic and adult stem and progenitor cells have been tried with conflicting outcome for cell therapy of central nervous system injury and diseases. Cellular characteristics and functional plasticity of Globose basal stem cells (GBCs) residing in the olfactory epithelium of rat olfactory mucosa have not been studied in the past by the neuroscientists due to unavailability of specific markers for GBCs. In the present research, we standardized some techniques to isolate GBCs from rat olfactory epithelium in pure form using a highly selective GBC-III antibody passaged through fluorescence activated cell sorter (FACS). We also characterized these cells immunohistologically using various pluripotent stem cell markers. This work also throws some light on ionic channels present on these stem cells which are responsible for their neuron induction potential. MATERIALS AND METHODS: Globose basal stem cells were isolated from rat olfactory epithelium using GBC-III antibody and were characterized as multipotent stem cells using various neural progenitor markers. Ionic channels on GBCs were studied with voltage clamping. RESULTS: GBCs could be isolated in pure (99% purity) form and were found to be stained positive for all neural progenitor cell markers. Voltage gated Na(+) channels were completely absent, which proves the unexcitable nature of GBCs. Leaky K(+) channels were found to be present on the GBC which was of no significance. CONCLUSION: This research work can be helpful in understanding the nature of these stem cells and utilising them in future as potent candidates for neuro-regenerative therapies.
format Online
Article
Text
id pubmed-4046242
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Mashhad University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-40462422014-06-05 Immunohistological and electrophysiological characterization of Globose basal stem cells Thakur, Avinash Muniswami, Duraimurugan Tharion, George Kanakasabapathy, Indirani Iran J Basic Med Sci Original Article OBJECTIVE(S): In the past few decades, variety of foetal, embryonic and adult stem and progenitor cells have been tried with conflicting outcome for cell therapy of central nervous system injury and diseases. Cellular characteristics and functional plasticity of Globose basal stem cells (GBCs) residing in the olfactory epithelium of rat olfactory mucosa have not been studied in the past by the neuroscientists due to unavailability of specific markers for GBCs. In the present research, we standardized some techniques to isolate GBCs from rat olfactory epithelium in pure form using a highly selective GBC-III antibody passaged through fluorescence activated cell sorter (FACS). We also characterized these cells immunohistologically using various pluripotent stem cell markers. This work also throws some light on ionic channels present on these stem cells which are responsible for their neuron induction potential. MATERIALS AND METHODS: Globose basal stem cells were isolated from rat olfactory epithelium using GBC-III antibody and were characterized as multipotent stem cells using various neural progenitor markers. Ionic channels on GBCs were studied with voltage clamping. RESULTS: GBCs could be isolated in pure (99% purity) form and were found to be stained positive for all neural progenitor cell markers. Voltage gated Na(+) channels were completely absent, which proves the unexcitable nature of GBCs. Leaky K(+) channels were found to be present on the GBC which was of no significance. CONCLUSION: This research work can be helpful in understanding the nature of these stem cells and utilising them in future as potent candidates for neuro-regenerative therapies. Mashhad University of Medical Sciences 2014-04 /pmc/articles/PMC4046242/ /pubmed/24904721 Text en Copyright: © Journal Management System. Created by sinaweb http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Thakur, Avinash
Muniswami, Duraimurugan
Tharion, George
Kanakasabapathy, Indirani
Immunohistological and electrophysiological characterization of Globose basal stem cells
title Immunohistological and electrophysiological characterization of Globose basal stem cells
title_full Immunohistological and electrophysiological characterization of Globose basal stem cells
title_fullStr Immunohistological and electrophysiological characterization of Globose basal stem cells
title_full_unstemmed Immunohistological and electrophysiological characterization of Globose basal stem cells
title_short Immunohistological and electrophysiological characterization of Globose basal stem cells
title_sort immunohistological and electrophysiological characterization of globose basal stem cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046242/
https://www.ncbi.nlm.nih.gov/pubmed/24904721
work_keys_str_mv AT thakuravinash immunohistologicalandelectrophysiologicalcharacterizationofglobosebasalstemcells
AT muniswamiduraimurugan immunohistologicalandelectrophysiologicalcharacterizationofglobosebasalstemcells
AT thariongeorge immunohistologicalandelectrophysiologicalcharacterizationofglobosebasalstemcells
AT kanakasabapathyindirani immunohistologicalandelectrophysiologicalcharacterizationofglobosebasalstemcells