Cargando…

Genome sequencing of high-penicillin producing industrial strain of Penicillium chrysogenum

BACKGROUND: Due to the importance of Penicillium chrysogenum holding in medicine, the genome of low-penicillin producing laboratorial strain Wisconsin54-1255 had been sequenced and fully annotated. Through classical mutagenesis of Wisconsin54-1255, product titers and productivities of penicillin hav...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Fu-Qiang, Zhong, Jun, Zhao, Ying, Xiao, Jingfa, Liu, Jing, Dai, Meng, Zheng, Guizhen, Zhang, Li, Yu, Jun, Wu, Jiayan, Duan, Baoling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046689/
https://www.ncbi.nlm.nih.gov/pubmed/24564352
http://dx.doi.org/10.1186/1471-2164-15-S1-S11
_version_ 1782480299782832128
author Wang, Fu-Qiang
Zhong, Jun
Zhao, Ying
Xiao, Jingfa
Liu, Jing
Dai, Meng
Zheng, Guizhen
Zhang, Li
Yu, Jun
Wu, Jiayan
Duan, Baoling
author_facet Wang, Fu-Qiang
Zhong, Jun
Zhao, Ying
Xiao, Jingfa
Liu, Jing
Dai, Meng
Zheng, Guizhen
Zhang, Li
Yu, Jun
Wu, Jiayan
Duan, Baoling
author_sort Wang, Fu-Qiang
collection PubMed
description BACKGROUND: Due to the importance of Penicillium chrysogenum holding in medicine, the genome of low-penicillin producing laboratorial strain Wisconsin54-1255 had been sequenced and fully annotated. Through classical mutagenesis of Wisconsin54-1255, product titers and productivities of penicillin have dramatically increased, but what underlying genome structural variations is still little known. Therefore, genome sequencing of a high-penicillin producing industrial strain is very meaningful. RESULTS: To reveal more insights into the genome structural variations of high-penicillin producing strain, we sequenced an industrial strain P. chrysogenum NCPC10086. By whole genome comparative analysis, we observed a large number of mutations, insertions and deletions, and structural variations. There are 69 new genes that not exist in the genome sequence of Wisconsin54-1255 and some of them are involved in energy metabolism, nitrogen metabolism and glutathione metabolism. Most importantly, we discovered a 53.7 Kb "new shift fragment" in a seven copies of determinative penicillin biosynthesis cluster in NCPC10086 and the arrangement type of amplified region is unique. Moreover, we presented two large-scale translocations in NCPC10086, containing genes involved energy, nitrogen metabolism and peroxysome pathway. At last, we found some non-synonymous mutations in the genes participating in homogentisate pathway or working as regulators of penicillin biosynthesis. CONCLUSIONS: We provided the first high-quality genome sequence of industrial high-penicillin strain of P. chrysogenum and carried out a comparative genome analysis with a low-producing experimental strain. The genomic variations we discovered are related with energy metabolism, nitrogen metabolism and so on. These findings demonstrate the potential information for insights into the high-penicillin yielding mechanism and metabolic engineering in the future. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-S1-S11) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4046689
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-40466892014-06-06 Genome sequencing of high-penicillin producing industrial strain of Penicillium chrysogenum Wang, Fu-Qiang Zhong, Jun Zhao, Ying Xiao, Jingfa Liu, Jing Dai, Meng Zheng, Guizhen Zhang, Li Yu, Jun Wu, Jiayan Duan, Baoling BMC Genomics Proceedings BACKGROUND: Due to the importance of Penicillium chrysogenum holding in medicine, the genome of low-penicillin producing laboratorial strain Wisconsin54-1255 had been sequenced and fully annotated. Through classical mutagenesis of Wisconsin54-1255, product titers and productivities of penicillin have dramatically increased, but what underlying genome structural variations is still little known. Therefore, genome sequencing of a high-penicillin producing industrial strain is very meaningful. RESULTS: To reveal more insights into the genome structural variations of high-penicillin producing strain, we sequenced an industrial strain P. chrysogenum NCPC10086. By whole genome comparative analysis, we observed a large number of mutations, insertions and deletions, and structural variations. There are 69 new genes that not exist in the genome sequence of Wisconsin54-1255 and some of them are involved in energy metabolism, nitrogen metabolism and glutathione metabolism. Most importantly, we discovered a 53.7 Kb "new shift fragment" in a seven copies of determinative penicillin biosynthesis cluster in NCPC10086 and the arrangement type of amplified region is unique. Moreover, we presented two large-scale translocations in NCPC10086, containing genes involved energy, nitrogen metabolism and peroxysome pathway. At last, we found some non-synonymous mutations in the genes participating in homogentisate pathway or working as regulators of penicillin biosynthesis. CONCLUSIONS: We provided the first high-quality genome sequence of industrial high-penicillin strain of P. chrysogenum and carried out a comparative genome analysis with a low-producing experimental strain. The genomic variations we discovered are related with energy metabolism, nitrogen metabolism and so on. These findings demonstrate the potential information for insights into the high-penicillin yielding mechanism and metabolic engineering in the future. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-S1-S11) contains supplementary material, which is available to authorized users. BioMed Central 2014-01-24 /pmc/articles/PMC4046689/ /pubmed/24564352 http://dx.doi.org/10.1186/1471-2164-15-S1-S11 Text en © Wang et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Proceedings
Wang, Fu-Qiang
Zhong, Jun
Zhao, Ying
Xiao, Jingfa
Liu, Jing
Dai, Meng
Zheng, Guizhen
Zhang, Li
Yu, Jun
Wu, Jiayan
Duan, Baoling
Genome sequencing of high-penicillin producing industrial strain of Penicillium chrysogenum
title Genome sequencing of high-penicillin producing industrial strain of Penicillium chrysogenum
title_full Genome sequencing of high-penicillin producing industrial strain of Penicillium chrysogenum
title_fullStr Genome sequencing of high-penicillin producing industrial strain of Penicillium chrysogenum
title_full_unstemmed Genome sequencing of high-penicillin producing industrial strain of Penicillium chrysogenum
title_short Genome sequencing of high-penicillin producing industrial strain of Penicillium chrysogenum
title_sort genome sequencing of high-penicillin producing industrial strain of penicillium chrysogenum
topic Proceedings
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046689/
https://www.ncbi.nlm.nih.gov/pubmed/24564352
http://dx.doi.org/10.1186/1471-2164-15-S1-S11
work_keys_str_mv AT wangfuqiang genomesequencingofhighpenicillinproducingindustrialstrainofpenicilliumchrysogenum
AT zhongjun genomesequencingofhighpenicillinproducingindustrialstrainofpenicilliumchrysogenum
AT zhaoying genomesequencingofhighpenicillinproducingindustrialstrainofpenicilliumchrysogenum
AT xiaojingfa genomesequencingofhighpenicillinproducingindustrialstrainofpenicilliumchrysogenum
AT liujing genomesequencingofhighpenicillinproducingindustrialstrainofpenicilliumchrysogenum
AT daimeng genomesequencingofhighpenicillinproducingindustrialstrainofpenicilliumchrysogenum
AT zhengguizhen genomesequencingofhighpenicillinproducingindustrialstrainofpenicilliumchrysogenum
AT zhangli genomesequencingofhighpenicillinproducingindustrialstrainofpenicilliumchrysogenum
AT yujun genomesequencingofhighpenicillinproducingindustrialstrainofpenicilliumchrysogenum
AT wujiayan genomesequencingofhighpenicillinproducingindustrialstrainofpenicilliumchrysogenum
AT duanbaoling genomesequencingofhighpenicillinproducingindustrialstrainofpenicilliumchrysogenum