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High-density single-molecule analysis of cell surface dynamics in C. elegans embryos

We describe a general, versatile and non-invasive method to image single molecules near the cell surface that can be applied to any GFP-tagged protein in C. elegans embryos. We exploit tunable expression via RNAi and a dynamically exchanging monomer pool to achieve fast continuous single-molecule im...

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Detalles Bibliográficos
Autores principales:	Robin, Francois B., McFadden, William M., Yao, Baixue, Munro, Edwin M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046709/ https://www.ncbi.nlm.nih.gov/pubmed/24727651 http://dx.doi.org/10.1038/nmeth.2928

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