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OsHUS1 Facilitates Accurate Meiotic Recombination in Rice
Meiotic recombination normally takes place between allelic sequences on homologs. This process can also occur between non-allelic homologous sequences. Such ectopic interaction events can lead to chromosome rearrangements and are normally avoided. However, much remains unknown about how these ectopi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046934/ https://www.ncbi.nlm.nih.gov/pubmed/24901798 http://dx.doi.org/10.1371/journal.pgen.1004405 |
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author | Che, Lixiao Wang, Kejian Tang, Ding Liu, Qiaoquan Chen, Xiaojun Li, Yafei Hu, Qing Shen, Yi Yu, Hengxiu Gu, Minghong Cheng, Zhukuan |
author_facet | Che, Lixiao Wang, Kejian Tang, Ding Liu, Qiaoquan Chen, Xiaojun Li, Yafei Hu, Qing Shen, Yi Yu, Hengxiu Gu, Minghong Cheng, Zhukuan |
author_sort | Che, Lixiao |
collection | PubMed |
description | Meiotic recombination normally takes place between allelic sequences on homologs. This process can also occur between non-allelic homologous sequences. Such ectopic interaction events can lead to chromosome rearrangements and are normally avoided. However, much remains unknown about how these ectopic interaction events are sensed and eliminated. In this study, using a screen in rice, we characterized a homolog of HUS1 and explored its function in meiotic recombination. In Oshus1 mutants, in conjunction with nearly normal homologous pairing and synapsis, vigorous, aberrant ectopic interactions occurred between nonhomologous chromosomes, leading to multivalent formation and subsequent chromosome fragmentation. These ectopic interactions relied on programed meiotic double strand breaks and were formed in a manner independent of the OsMER3-mediated interference-sensitive crossover pathway. Although early homologous recombination events occurred normally, the number of interference-sensitive crossovers was reduced in the absence of OsHUS1. Together, our results indicate that OsHUS1 might be involved in regulating ectopic interactions during meiosis, probably by forming the canonical RAD9-RAD1-HUS1 (9-1-1) complex. |
format | Online Article Text |
id | pubmed-4046934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40469342014-06-09 OsHUS1 Facilitates Accurate Meiotic Recombination in Rice Che, Lixiao Wang, Kejian Tang, Ding Liu, Qiaoquan Chen, Xiaojun Li, Yafei Hu, Qing Shen, Yi Yu, Hengxiu Gu, Minghong Cheng, Zhukuan PLoS Genet Research Article Meiotic recombination normally takes place between allelic sequences on homologs. This process can also occur between non-allelic homologous sequences. Such ectopic interaction events can lead to chromosome rearrangements and are normally avoided. However, much remains unknown about how these ectopic interaction events are sensed and eliminated. In this study, using a screen in rice, we characterized a homolog of HUS1 and explored its function in meiotic recombination. In Oshus1 mutants, in conjunction with nearly normal homologous pairing and synapsis, vigorous, aberrant ectopic interactions occurred between nonhomologous chromosomes, leading to multivalent formation and subsequent chromosome fragmentation. These ectopic interactions relied on programed meiotic double strand breaks and were formed in a manner independent of the OsMER3-mediated interference-sensitive crossover pathway. Although early homologous recombination events occurred normally, the number of interference-sensitive crossovers was reduced in the absence of OsHUS1. Together, our results indicate that OsHUS1 might be involved in regulating ectopic interactions during meiosis, probably by forming the canonical RAD9-RAD1-HUS1 (9-1-1) complex. Public Library of Science 2014-06-05 /pmc/articles/PMC4046934/ /pubmed/24901798 http://dx.doi.org/10.1371/journal.pgen.1004405 Text en © 2014 Che et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Che, Lixiao Wang, Kejian Tang, Ding Liu, Qiaoquan Chen, Xiaojun Li, Yafei Hu, Qing Shen, Yi Yu, Hengxiu Gu, Minghong Cheng, Zhukuan OsHUS1 Facilitates Accurate Meiotic Recombination in Rice |
title | OsHUS1 Facilitates Accurate Meiotic Recombination in Rice |
title_full | OsHUS1 Facilitates Accurate Meiotic Recombination in Rice |
title_fullStr | OsHUS1 Facilitates Accurate Meiotic Recombination in Rice |
title_full_unstemmed | OsHUS1 Facilitates Accurate Meiotic Recombination in Rice |
title_short | OsHUS1 Facilitates Accurate Meiotic Recombination in Rice |
title_sort | oshus1 facilitates accurate meiotic recombination in rice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046934/ https://www.ncbi.nlm.nih.gov/pubmed/24901798 http://dx.doi.org/10.1371/journal.pgen.1004405 |
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