Cargando…

A PCR Based Protocol for Detecting Indel Mutations Induced by TALENs and CRISPR/Cas9 in Zebrafish

Genome editing techniques such as the zinc-finger nucleases (ZFNs), transcription activator-like effecter nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system Cas9 can induce efficient DNA double strand breaks (DSBs) at the target g...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yu, Chuan, Zhang, Yaguang, Yao, Shaohua, Wei, Yuquan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046980/ https://www.ncbi.nlm.nih.gov/pubmed/24901507 http://dx.doi.org/10.1371/journal.pone.0098282

Ejemplares similares

Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish
por: Xiao, An, et al.
Publicado: (2013)

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing
por: Montague, Tessa G., et al.
Publicado: (2014)

RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency
por: Song, Jun, et al.
Publicado: (2016)

A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
por: Sakurai, Takayuki, et al.
Publicado: (2014)

Genome-wide specificity of plant genome editing by both CRISPR–Cas9 and TALEN
por: Bessoltane, Nadia, et al.
Publicado: (2022)

Precision targeting tumor cells using cancer-specific InDel mutations with CRISPR-Cas9
por: Kwon, Taejoon, et al.
Publicado: (2022)

Discrepancies in indel software resolution with somatic CRISPR/Cas9 tumorigenesis models
por: Brockman, Qierra R., et al.
Publicado: (2023)

TALEN and CRISPR/Cas Genome Editing Systems: Tools of Discovery
por: Nemudryi, A. A., et al.
Publicado: (2014)

Various applications of TALEN- and CRISPR/Cas9-mediated homologous recombination to modify the Drosophila genome
por: Yu, Zhongsheng, et al.
Publicado: (2014)

Genomic Access to Monarch Migration Using TALEN and CRISPR/Cas9-Mediated Targeted Mutagenesis
por: Markert, Matthew J., et al.
Publicado: (2016)

Genome-wide Specificity of Highly Efficient TALENs and CRISPR/Cas9 for T Cell Receptor Modification
por: Knipping, Friederike, et al.
Publicado: (2017)

CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
por: Amorim, João Pedro, et al.
Publicado: (2020)

CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula
por: Curtin, Shaun J., et al.
Publicado: (2017)

CRISPR-induced indels and base editing using the Staphylococcus aureus Cas9 in potato
por: Veillet, Florian, et al.
Publicado: (2020)

Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9
por: Prykhozhij, Sergey V, et al.
Publicado: (2018)

Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9
por: Prykhozhij, Sergey V, et al.
Publicado: (2018)

A simple genotyping method to detect small CRISPR-Cas9 induced indels by agarose gel electrophoresis
por: Bhattacharya, Debanjan, et al.
Publicado: (2019)

Genome Editing With TALEN, CRISPR-Cas9 and CRISPR-Cas12a in Combination With AAV6 Homology Donor Restores T Cell Function for XLP
por: Houghton, Benjamin C., et al.
Publicado: (2022)

Testing the causality between CYP9M10 and pyrethroid resistance using the TALEN and CRISPR/Cas9 technologies
por: Itokawa, Kentaro, et al.
Publicado: (2016)

Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9
por: Sato, Takuya, et al.
Publicado: (2015)

Comparison of CRISPR/Cas9 and TALENs on editing an integrated EGFP gene in the genome of HEK293FT cells
por: He, Zuyong, et al.
Publicado: (2016)

Disruption of miRNA sequences by TALENs and CRISPR/Cas9 induces varied lengths of miRNA production
por: Bi, Honghao, et al.
Publicado: (2019)

TALEN outperforms Cas9 in editing heterochromatin target sites
por: Jain, Surbhi, et al.
Publicado: (2021)

Both TALENs and CRISPR/Cas9 directly target the HBB IVS2–654 (C > T) mutation in β-thalassemia-derived iPSCs
por: Xu, Peng, et al.
Publicado: (2015)

Challenges associated with homologous directed repair using CRISPR-Cas9 and TALEN to edit the DMD genetic mutation in canine Duchenne muscular dystrophy
por: Mata López, Sara, et al.
Publicado: (2020)

CRISPR-Cas9-induced gene knockout in zebrafish
por: Medishetti, Raghavender, et al.
Publicado: (2022)

Rapid generation of drug-resistance alleles at endogenous loci using CRISPR-Cas9 indel mutagenesis
por: Ipsaro, Jonathan J., et al.
Publicado: (2017)

Prediction of CRISPR-Cas9 off-target activities with mismatches and indels based on hybrid neural network
por: Yang, Yanpeng, et al.
Publicado: (2023)

Controlled delivery of β-globin-targeting TALENs and CRISPR/Cas9 into mammalian cells for genome editing using microinjection
por: Cottle, Renee N., et al.
Publicado: (2015)

Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9
por: Nakade, Shota, et al.
Publicado: (2014)

Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene
por: Nerys-Junior, Arildo, et al.
Publicado: (2018)

Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome
por: Dahlem, Timothy J., et al.
Publicado: (2012)

Correction: Challenges associated with homologous directed repair using CRISPR-Cas9 and TALEN to edit the DMD genetic mutation in canine Duchenne muscular dystrophy
por: Mata López, Sara, et al.
Publicado: (2020)

Knockout of Zebrafish Ovarian Aromatase Gene (cyp19a1a) by TALEN and CRISPR/Cas9 Leads to All-male Offspring Due to Failed Ovarian Differentiation
por: Lau, Esther Shuk-Wa, et al.
Publicado: (2016)

Plant genome editing with TALEN and CRISPR
por: Malzahn, Aimee, et al.
Publicado: (2017)

Digital droplet PCR and IDAA for the detection of CRISPR indel edits in the malaria species Anopheles stephensi
por: Carballar-Lejarazú, Rebeca, et al.
Publicado: (2020)

Heritable Genomic Fragment Deletions and Small Indels in the Putative ENGase Gene Induced by CRISPR/Cas9 in Barley
por: Kapusi, Eszter, et al.
Publicado: (2017)

Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9
por: Feng, Yan, et al.
Publicado: (2016)

Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9
por: Li, Hongmei Lisa, et al.
Publicado: (2014)

Comparative Analysis of piggyBac, CRISPR/Cas9 and TALEN Mediated BAC Transgenesis in the Zygote for the Generation of Humanized SIRPA Rats
por: Jung, Chris J., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_18e26lq7mk2vbl4cnm915utb89