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From Laboratory to Field: OsNRAMP5-Knockdown Rice Is a Promising Candidate for Cd Phytoremediation in Paddy Fields

Previously, we reported that OsNRAMP5 functions as a manganese, iron, and cadmium (Cd) transporter. The shoot Cd content in OsNRAMP5 RNAi plants was higher than that in wild-type (WT) plants, whereas the total Cd content (roots plus shoots) was lower. For efficient Cd phytoremediation, we produced O...

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Autores principales: Takahashi, Ryuichi, Ishimaru, Yasuhiro, Shimo, Hugo, Bashir, Khurram, Senoura, Takeshi, Sugimoto, Kazuhiko, Ono, Kazuko, Suzui, Nobuo, Kawachi, Naoki, Ishii, Satomi, Yin, Yong-Gen, Fujimaki, Shu, Yano, Masahiro, Nishizawa, Naoko K., Nakanishi, Hiromi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4047016/
https://www.ncbi.nlm.nih.gov/pubmed/24901230
http://dx.doi.org/10.1371/journal.pone.0098816
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author Takahashi, Ryuichi
Ishimaru, Yasuhiro
Shimo, Hugo
Bashir, Khurram
Senoura, Takeshi
Sugimoto, Kazuhiko
Ono, Kazuko
Suzui, Nobuo
Kawachi, Naoki
Ishii, Satomi
Yin, Yong-Gen
Fujimaki, Shu
Yano, Masahiro
Nishizawa, Naoko K.
Nakanishi, Hiromi
author_facet Takahashi, Ryuichi
Ishimaru, Yasuhiro
Shimo, Hugo
Bashir, Khurram
Senoura, Takeshi
Sugimoto, Kazuhiko
Ono, Kazuko
Suzui, Nobuo
Kawachi, Naoki
Ishii, Satomi
Yin, Yong-Gen
Fujimaki, Shu
Yano, Masahiro
Nishizawa, Naoko K.
Nakanishi, Hiromi
author_sort Takahashi, Ryuichi
collection PubMed
description Previously, we reported that OsNRAMP5 functions as a manganese, iron, and cadmium (Cd) transporter. The shoot Cd content in OsNRAMP5 RNAi plants was higher than that in wild-type (WT) plants, whereas the total Cd content (roots plus shoots) was lower. For efficient Cd phytoremediation, we produced OsNRAMP5 RNAi plants using the natural high Cd-accumulating cultivar Anjana Dhan (A5i). Using a positron-emitting tracer imaging system, we assessed the time-course of Cd absorption and accumulation in A5i plants. Enhanced (107)Cd translocation from the roots to the shoots was observed in A5i plants. To evaluate the phytoremediation capability of A5i plants, we performed a field experiment in a Cd-contaminated paddy field. The biomass of the A5i plants was unchanged by the suppression of OsNRAMP5 expression; the A5i plants accumulated twice as much Cd in their shoots as WT plants. Thus, A5i plants could be used for rapid Cd extraction and the efficient phytoremediation of Cd from paddy fields, leading to safer food production.
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spelling pubmed-40470162014-06-09 From Laboratory to Field: OsNRAMP5-Knockdown Rice Is a Promising Candidate for Cd Phytoremediation in Paddy Fields Takahashi, Ryuichi Ishimaru, Yasuhiro Shimo, Hugo Bashir, Khurram Senoura, Takeshi Sugimoto, Kazuhiko Ono, Kazuko Suzui, Nobuo Kawachi, Naoki Ishii, Satomi Yin, Yong-Gen Fujimaki, Shu Yano, Masahiro Nishizawa, Naoko K. Nakanishi, Hiromi PLoS One Research Article Previously, we reported that OsNRAMP5 functions as a manganese, iron, and cadmium (Cd) transporter. The shoot Cd content in OsNRAMP5 RNAi plants was higher than that in wild-type (WT) plants, whereas the total Cd content (roots plus shoots) was lower. For efficient Cd phytoremediation, we produced OsNRAMP5 RNAi plants using the natural high Cd-accumulating cultivar Anjana Dhan (A5i). Using a positron-emitting tracer imaging system, we assessed the time-course of Cd absorption and accumulation in A5i plants. Enhanced (107)Cd translocation from the roots to the shoots was observed in A5i plants. To evaluate the phytoremediation capability of A5i plants, we performed a field experiment in a Cd-contaminated paddy field. The biomass of the A5i plants was unchanged by the suppression of OsNRAMP5 expression; the A5i plants accumulated twice as much Cd in their shoots as WT plants. Thus, A5i plants could be used for rapid Cd extraction and the efficient phytoremediation of Cd from paddy fields, leading to safer food production. Public Library of Science 2014-06-05 /pmc/articles/PMC4047016/ /pubmed/24901230 http://dx.doi.org/10.1371/journal.pone.0098816 Text en © 2014 Takahashi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Takahashi, Ryuichi
Ishimaru, Yasuhiro
Shimo, Hugo
Bashir, Khurram
Senoura, Takeshi
Sugimoto, Kazuhiko
Ono, Kazuko
Suzui, Nobuo
Kawachi, Naoki
Ishii, Satomi
Yin, Yong-Gen
Fujimaki, Shu
Yano, Masahiro
Nishizawa, Naoko K.
Nakanishi, Hiromi
From Laboratory to Field: OsNRAMP5-Knockdown Rice Is a Promising Candidate for Cd Phytoremediation in Paddy Fields
title From Laboratory to Field: OsNRAMP5-Knockdown Rice Is a Promising Candidate for Cd Phytoremediation in Paddy Fields
title_full From Laboratory to Field: OsNRAMP5-Knockdown Rice Is a Promising Candidate for Cd Phytoremediation in Paddy Fields
title_fullStr From Laboratory to Field: OsNRAMP5-Knockdown Rice Is a Promising Candidate for Cd Phytoremediation in Paddy Fields
title_full_unstemmed From Laboratory to Field: OsNRAMP5-Knockdown Rice Is a Promising Candidate for Cd Phytoremediation in Paddy Fields
title_short From Laboratory to Field: OsNRAMP5-Knockdown Rice Is a Promising Candidate for Cd Phytoremediation in Paddy Fields
title_sort from laboratory to field: osnramp5-knockdown rice is a promising candidate for cd phytoremediation in paddy fields
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4047016/
https://www.ncbi.nlm.nih.gov/pubmed/24901230
http://dx.doi.org/10.1371/journal.pone.0098816
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