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Pulsed and Continuous Ultrasound Increase Chondrogenesis through the Increase of Heat Shock Protein 70 Expression in Rat Articular Cartilage

[Purpose] The present study was aimed to investigate the effects of pulsed and continuous ultrasound (US) irradiation on heat shock protein (HSP) 70 and mRNA levels of chondrogenesis-related gene expression in rat tibial articular cartilage. [Subjects and Methods] Forty-eight rats with body weights...

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Autores principales: Nam, Ki Won, Seo, Dong Yel, Kim, Min Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society of Physical Therapy Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4047224/
https://www.ncbi.nlm.nih.gov/pubmed/24926124
http://dx.doi.org/10.1589/jpts.26.647
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author Nam, Ki Won
Seo, Dong Yel
Kim, Min Hee
author_facet Nam, Ki Won
Seo, Dong Yel
Kim, Min Hee
author_sort Nam, Ki Won
collection PubMed
description [Purpose] The present study was aimed to investigate the effects of pulsed and continuous ultrasound (US) irradiation on heat shock protein (HSP) 70 and mRNA levels of chondrogenesis-related gene expression in rat tibial articular cartilage. [Subjects and Methods] Forty-eight rats with body weights of 200−250 g were randomly divided into three groups. In the control (CON) group, three rats were treated with sham sonication. The pulsed US irradiation group was irradiated with a pulse rate of 20%, a frequency of 1 MHz, and an intensity of 1.5 W/cm(2) for 10 minutes. The continuous US irradiation group was continuously with a frequency of 1 MHz and an intensity of 1.5 W/cm(2) for 10 minutes. Immunohistochemistry for evaluation of HSP 70 and RT-PCR for expression of the chondrogenesis-related mRNA were used. [Results] The expression of HSP70 protein was increased in the pulsed and continuous US groups. The increase in the continuous US group was more prominent than in the pulsed US group. In addition, pulsed and continuous US irradiation increased the expression of Mustn1 and Sox9. [Conclusion] The results of this study show that US increases chondrogenesis via the increase of HSP 70 and chondrogenesis-related mRNA expressions in rat articular cartilage.
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spelling pubmed-40472242014-06-12 Pulsed and Continuous Ultrasound Increase Chondrogenesis through the Increase of Heat Shock Protein 70 Expression in Rat Articular Cartilage Nam, Ki Won Seo, Dong Yel Kim, Min Hee J Phys Ther Sci Original [Purpose] The present study was aimed to investigate the effects of pulsed and continuous ultrasound (US) irradiation on heat shock protein (HSP) 70 and mRNA levels of chondrogenesis-related gene expression in rat tibial articular cartilage. [Subjects and Methods] Forty-eight rats with body weights of 200−250 g were randomly divided into three groups. In the control (CON) group, three rats were treated with sham sonication. The pulsed US irradiation group was irradiated with a pulse rate of 20%, a frequency of 1 MHz, and an intensity of 1.5 W/cm(2) for 10 minutes. The continuous US irradiation group was continuously with a frequency of 1 MHz and an intensity of 1.5 W/cm(2) for 10 minutes. Immunohistochemistry for evaluation of HSP 70 and RT-PCR for expression of the chondrogenesis-related mRNA were used. [Results] The expression of HSP70 protein was increased in the pulsed and continuous US groups. The increase in the continuous US group was more prominent than in the pulsed US group. In addition, pulsed and continuous US irradiation increased the expression of Mustn1 and Sox9. [Conclusion] The results of this study show that US increases chondrogenesis via the increase of HSP 70 and chondrogenesis-related mRNA expressions in rat articular cartilage. The Society of Physical Therapy Science 2014-05-29 2014-05 /pmc/articles/PMC4047224/ /pubmed/24926124 http://dx.doi.org/10.1589/jpts.26.647 Text en 2014©by the Society of Physical Therapy Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original
Nam, Ki Won
Seo, Dong Yel
Kim, Min Hee
Pulsed and Continuous Ultrasound Increase Chondrogenesis through the Increase of Heat Shock Protein 70 Expression in Rat Articular Cartilage
title Pulsed and Continuous Ultrasound Increase Chondrogenesis through the Increase of Heat Shock Protein 70 Expression in Rat Articular Cartilage
title_full Pulsed and Continuous Ultrasound Increase Chondrogenesis through the Increase of Heat Shock Protein 70 Expression in Rat Articular Cartilage
title_fullStr Pulsed and Continuous Ultrasound Increase Chondrogenesis through the Increase of Heat Shock Protein 70 Expression in Rat Articular Cartilage
title_full_unstemmed Pulsed and Continuous Ultrasound Increase Chondrogenesis through the Increase of Heat Shock Protein 70 Expression in Rat Articular Cartilage
title_short Pulsed and Continuous Ultrasound Increase Chondrogenesis through the Increase of Heat Shock Protein 70 Expression in Rat Articular Cartilage
title_sort pulsed and continuous ultrasound increase chondrogenesis through the increase of heat shock protein 70 expression in rat articular cartilage
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4047224/
https://www.ncbi.nlm.nih.gov/pubmed/24926124
http://dx.doi.org/10.1589/jpts.26.647
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