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Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication

The genetic information encoded in genomes must be faithfully replicated and transmitted to daughter cells. The recent discovery of consecutive DNA conversions by TET family proteins of 5-methylcytosine into 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine (5caC) suggests these modi...

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Autores principales: Shibutani, Toshihiro, Ito, Shinsuke, Toda, Mariko, Kanao, Rie, Collins, Leonard B., Shibata, Marika, Urabe, Miho, Koseki, Haruhiko, Masuda, Yuji, Swenberg, James A., Masutani, Chikahide, Hanaoka, Fumio, Iwai, Shigenori, Kuraoka, Isao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4048885/
https://www.ncbi.nlm.nih.gov/pubmed/24910358
http://dx.doi.org/10.1038/srep05220
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author Shibutani, Toshihiro
Ito, Shinsuke
Toda, Mariko
Kanao, Rie
Collins, Leonard B.
Shibata, Marika
Urabe, Miho
Koseki, Haruhiko
Masuda, Yuji
Swenberg, James A.
Masutani, Chikahide
Hanaoka, Fumio
Iwai, Shigenori
Kuraoka, Isao
author_facet Shibutani, Toshihiro
Ito, Shinsuke
Toda, Mariko
Kanao, Rie
Collins, Leonard B.
Shibata, Marika
Urabe, Miho
Koseki, Haruhiko
Masuda, Yuji
Swenberg, James A.
Masutani, Chikahide
Hanaoka, Fumio
Iwai, Shigenori
Kuraoka, Isao
author_sort Shibutani, Toshihiro
collection PubMed
description The genetic information encoded in genomes must be faithfully replicated and transmitted to daughter cells. The recent discovery of consecutive DNA conversions by TET family proteins of 5-methylcytosine into 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine (5caC) suggests these modified cytosines act as DNA lesions, which could threaten genome integrity. Here, we have shown that although 5caC pairs with guanine during DNA replication in vitro, G·5caC pairs stimulated DNA polymerase exonuclease activity and were recognized by the mismatch repair (MMR) proteins. Knockdown of thymine DNA glycosylase increased 5caC in genome, affected cell proliferation via MMR, indicating MMR is a novel reader for 5caC. These results suggest the epigenetic modification products of 5caC behave as DNA lesions.
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spelling pubmed-40488852014-06-12 Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication Shibutani, Toshihiro Ito, Shinsuke Toda, Mariko Kanao, Rie Collins, Leonard B. Shibata, Marika Urabe, Miho Koseki, Haruhiko Masuda, Yuji Swenberg, James A. Masutani, Chikahide Hanaoka, Fumio Iwai, Shigenori Kuraoka, Isao Sci Rep Article The genetic information encoded in genomes must be faithfully replicated and transmitted to daughter cells. The recent discovery of consecutive DNA conversions by TET family proteins of 5-methylcytosine into 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine (5caC) suggests these modified cytosines act as DNA lesions, which could threaten genome integrity. Here, we have shown that although 5caC pairs with guanine during DNA replication in vitro, G·5caC pairs stimulated DNA polymerase exonuclease activity and were recognized by the mismatch repair (MMR) proteins. Knockdown of thymine DNA glycosylase increased 5caC in genome, affected cell proliferation via MMR, indicating MMR is a novel reader for 5caC. These results suggest the epigenetic modification products of 5caC behave as DNA lesions. Nature Publishing Group 2014-06-09 /pmc/articles/PMC4048885/ /pubmed/24910358 http://dx.doi.org/10.1038/srep05220 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Article
Shibutani, Toshihiro
Ito, Shinsuke
Toda, Mariko
Kanao, Rie
Collins, Leonard B.
Shibata, Marika
Urabe, Miho
Koseki, Haruhiko
Masuda, Yuji
Swenberg, James A.
Masutani, Chikahide
Hanaoka, Fumio
Iwai, Shigenori
Kuraoka, Isao
Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication
title Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication
title_full Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication
title_fullStr Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication
title_full_unstemmed Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication
title_short Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication
title_sort guanine- 5-carboxylcytosine base pairs mimic mismatches during dna replication
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4048885/
https://www.ncbi.nlm.nih.gov/pubmed/24910358
http://dx.doi.org/10.1038/srep05220
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