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Specialized Transduction Designed for Precise High-Throughput Unmarked Deletions in Mycobacterium tuberculosis

Specialized transduction has proven to be useful for generating deletion mutants in most mycobacteria, including virulent Mycobacterium tuberculosis. We have improved this system by developing (i) a single-step strategy for the construction of allelic exchange substrates (AES), (ii) a temperature-se...

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Detalles Bibliográficos
Autores principales:	Jain, Paras, Hsu, Tsungda, Arai, Masayoshi, Biermann, Karolin, Thaler, David S., Nguyen, Andrew, González, Pablo A., Tufariello, Joann M., Kriakov, Jordan, Chen, Bing, Larsen, Michelle H., Jacobs, William R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society of Microbiology 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049104/ https://www.ncbi.nlm.nih.gov/pubmed/24895308 http://dx.doi.org/10.1128/mBio.01245-14

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