Cargando…

A novel, nested, multiplex, real-time PCR for detection of bacteria and fungi in blood

BACKGROUND: The study describes the application of the PCR method for the simultaneous detection of DNA of Gram-negative bacteria, Gram-positive bacteria, yeast fungi and filamentous fungi in blood and, thus, a whole range of microbial etiological agents that may cause sepsis. Material for the study...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gosiewski, Tomasz, Jurkiewicz-Badacz, Danuta, Sroka, Agnieszka, Brzychczy-Włoch, Monika, Bulanda, Małgorzata
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049433/ https://www.ncbi.nlm.nih.gov/pubmed/24893651 http://dx.doi.org/10.1186/1471-2180-14-144

Ejemplares similares

Comparison of nested, multiplex, qPCR; FISH; SeptiFast and blood culture methods in detection and identification of bacteria and fungi in blood of patients with sepsis
por: Gosiewski, Tomasz, et al.
Publicado: (2014)

Comparison of PCR, Fluorescent in Situ Hybridization and Blood Cultures for Detection of Bacteremia in Children and Adolescents During Antibiotic Therapy
por: ŹRÓDŁOWSKI, TOMASZ W., et al.
Publicado: (2018)

Multilocus Sequence Types of Invasive and Colonizing Neonatal Group B Streptococci in Poland
por: Brzychczy-Wloch, Monika, et al.
Publicado: (2014)

The application of multiplex PCR to detect seven different DNA targets in group B streptococci
por: Gosiewski, Tomasz, et al.
Publicado: (2012)

Comparison of Antigen Tests and qPCR in Rapid Diagnostics of Infections Caused by SARS-CoV-2 Virus
por: Klajmon, Adrianna, et al.
Publicado: (2021)

A study of the effects of therapeutic doses of ionizing radiation in vitro on Lactobacillus isolates originating from the vagina - a pilot study
por: Gosiewski, Tomasz, et al.
Publicado: (2016)

Comparison of Methods for Isolation of Bacterial and Fungal DNA from Human Blood
por: Gosiewski, Tomasz, et al.
Publicado: (2013)

The dynamics of vaginal and rectal Lactobacillus spp. flora in subsequent trimesters of pregnancy in healthy Polish women, assessed using the Sanger sequencing method
por: Dobrut, Anna, et al.
Publicado: (2018)

Quantitative evaluation of fungi of the genus Candida in the feces of adult patients with type 1 and 2 diabetes - a pilot study
por: Gosiewski, Tomasz, et al.
Publicado: (2014)

Deep learning approach to describe and classify fungi microscopic images
por: Zieliński, Bartosz, et al.
Publicado: (2020)

The application of genetics methods to differentiation of three Lactobacillus species of human origin
por: Gosiewski, Tomasz, et al.
Publicado: (2011)

Next-Generation Sequencing as a Tool to Detect Vaginal Microbiota Disturbances during Pregnancy
por: Sroka-Oleksiak, Agnieszka, et al.
Publicado: (2020)

A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus
por: Feng, Zhi-shan, et al.
Publicado: (2018)

Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR
por: Kuamsab, Napaporn, et al.
Publicado: (2012)

Clonal Dissemination of KPC-2, VIM-1, OXA-48-Producing Klebsiella pneumoniae ST147 in Katowice, Poland
por: OCHOŃSKA, DOROTA, et al.
Publicado: (2021)

Metagenomic Analysis of Duodenal Microbiota Reveals a Potential Biomarker of Dysbiosis in the Course of Obesity and Type 2 Diabetes: A Pilot Study
por: Sroka-Oleksiak, Agnieszka, et al.
Publicado: (2020)

Evidence for Infections by the Same Strain of Beta 2-toxigenic Clostridium perfringens Type A Acquired in One Hospital Ward
por: SALAMON, DOMINIKA, et al.
Publicado: (2019)

Improved detection of Escherichia coli and coliform bacteria by multiplex PCR
por: Molina, Felipe, et al.
Publicado: (2015)

Do NAAT-Based Methods Increase the Diagnostic Sensitivity of Streptococcus agalactiae Carriage Detection in Pregnant Women?
por: Sroka-Oleksiak, Agnieszka, et al.
Publicado: (2023)

Analysis of the Gut Mycobiome in Adult Patients with Type 1 and Type 2 Diabetes Using Next-Generation Sequencing (NGS) with Increased Sensitivity—Pilot Study
por: Salamon, Dominika, et al.
Publicado: (2021)

Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group
por: Vezenegho, Samuel B, et al.
Publicado: (2009)

Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR
por: Hernández-Arteaga, Socorro, et al.
Publicado: (2010)

A nested real-time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots
por: Tran, Tuan M, et al.
Publicado: (2014)

Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS)
por: Brzychczy- Sroka, Barbara, et al.
Publicado: (2023)

Epitopes of Immunoreactive Proteins of Streptococcus Agalactiae: Enolase, Inosine 5′-Monophosphate Dehydrogenase and Molecular Chaperone GroEL
por: Dobrut, Anna, et al.
Publicado: (2018)

Multiplex bisulfite PCR resequencing of clinical FFPE DNA
por: Korbie, Darren, et al.
Publicado: (2015)

Fast multiplex real-time PCR assay for simultaneous detection of dog and human blood and Leishmania parasites in sand flies
por: Sales, Kamila Gaudêncio da Silva, et al.
Publicado: (2020)

A TaqMan-based multiplex real-time PCR assay for the rapid detection of tigecycline resistance genes from bacteria, faeces and environmental samples
por: Li, Yiming, et al.
Publicado: (2020)

FungiQuant: A broad-coverage fungal quantitative real-time PCR assay
por: Liu, Cindy M, et al.
Publicado: (2012)

A novel, multiplex, real-time PCR–based approach for the detection of the commonly occurring pathogenic fungi and bacteria
por: Horváth, Ádám, et al.
Publicado: (2013)

Sample-ready multiplex qPCR assay for detection of malaria
por: Kamau, Edwin, et al.
Publicado: (2014)

Prevalence of antibiotic resistance in multi-drug resistant coagulase-negative staphylococci isolated from invasive infection in very low birth weight neonates in two Polish NICUs
por: Brzychczy-Wloch, Monika, et al.
Publicado: (2013)

Comparative Evaluation of LAMP and Nested PCR for the Rapid Diagnosis of Mycobacterium marinum Infection
por: Feng, Yumiao, et al.
Publicado: (2023)

Rapid and robust squashed spore/colony PCR of industrially important fungi
por: Yuan, Guoliang, et al.
Publicado: (2023)

A multiplex PCR for rapid identification of Brassica species in the triangle of U
por: Koh, Joshua C. O., et al.
Publicado: (2017)

Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping
por: Hayden, Matthew J, et al.
Publicado: (2008)

Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples
por: O'Regan, Edel, et al.
Publicado: (2008)

Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI
por: Bester, Rachelle, et al.
Publicado: (2012)

A multiplex PCR assay for six Aedini species, including Aedes albopictus
por: Bang, Woo Jun, et al.
Publicado: (2021)

The Gut Microbiota Profile According to Glycemic Control in Type 1 Diabetes Patients Treated with Personal Insulin Pumps
por: Mrozinska, Sandra, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_dm51nlvaios6ig6j9a29odu497