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Modulation of microRNA expression in human lung cancer cells by the G9a histone methyltransferase inhibitor BIX01294
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the expression of their target genes at the post-transcriptional level. In cancer cells, miRNAs, depending on the biological functions of their target genes, may have a tumor-promoting or -suppressing effect. Treatment of cancer cells with i...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049738/ https://www.ncbi.nlm.nih.gov/pubmed/24932239 http://dx.doi.org/10.3892/ol.2014.2034 |
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author | PANG, ALAN LAP-YIN TITLE, ALEXANDRA C. RENNERT, OWEN M. |
author_facet | PANG, ALAN LAP-YIN TITLE, ALEXANDRA C. RENNERT, OWEN M. |
author_sort | PANG, ALAN LAP-YIN |
collection | PubMed |
description | MicroRNAs (miRNAs) are small non-coding RNAs that regulate the expression of their target genes at the post-transcriptional level. In cancer cells, miRNAs, depending on the biological functions of their target genes, may have a tumor-promoting or -suppressing effect. Treatment of cancer cells with inhibitors of DNA methylation and/or histone deacetylation modulates the expression level of miRNAs, which provides evidence for epigenetic regulation of miRNA expression. The consequences of inhibition of histone methyltransferase on miRNA expression, however, have not been thoroughly investigated. The present study examined the expression pattern of miRNAs in the non-small cell lung cancer cell line, H1299 with or without treatment of BIX01294, a potent chemical inhibitor of G9a methyltransferase that catalyzes the mono-and di-methylation of the lysine 9 residue of histone H3. By coupling microarray analysis with quantitative real-time polymerase chain reaction analysis, two miRNAs were identified that showed consistent downregulation following BIX01294 treatment. The results indicate that histone H3 methylation regulates miRNA expression in lung cancer cells, which may provide additional insight for future chemical treatment of lung cancer. |
format | Online Article Text |
id | pubmed-4049738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-40497382014-06-13 Modulation of microRNA expression in human lung cancer cells by the G9a histone methyltransferase inhibitor BIX01294 PANG, ALAN LAP-YIN TITLE, ALEXANDRA C. RENNERT, OWEN M. Oncol Lett Articles MicroRNAs (miRNAs) are small non-coding RNAs that regulate the expression of their target genes at the post-transcriptional level. In cancer cells, miRNAs, depending on the biological functions of their target genes, may have a tumor-promoting or -suppressing effect. Treatment of cancer cells with inhibitors of DNA methylation and/or histone deacetylation modulates the expression level of miRNAs, which provides evidence for epigenetic regulation of miRNA expression. The consequences of inhibition of histone methyltransferase on miRNA expression, however, have not been thoroughly investigated. The present study examined the expression pattern of miRNAs in the non-small cell lung cancer cell line, H1299 with or without treatment of BIX01294, a potent chemical inhibitor of G9a methyltransferase that catalyzes the mono-and di-methylation of the lysine 9 residue of histone H3. By coupling microarray analysis with quantitative real-time polymerase chain reaction analysis, two miRNAs were identified that showed consistent downregulation following BIX01294 treatment. The results indicate that histone H3 methylation regulates miRNA expression in lung cancer cells, which may provide additional insight for future chemical treatment of lung cancer. D.A. Spandidos 2014-06 2014-04-04 /pmc/articles/PMC4049738/ /pubmed/24932239 http://dx.doi.org/10.3892/ol.2014.2034 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles PANG, ALAN LAP-YIN TITLE, ALEXANDRA C. RENNERT, OWEN M. Modulation of microRNA expression in human lung cancer cells by the G9a histone methyltransferase inhibitor BIX01294 |
title | Modulation of microRNA expression in human lung cancer cells by the G9a histone methyltransferase inhibitor BIX01294 |
title_full | Modulation of microRNA expression in human lung cancer cells by the G9a histone methyltransferase inhibitor BIX01294 |
title_fullStr | Modulation of microRNA expression in human lung cancer cells by the G9a histone methyltransferase inhibitor BIX01294 |
title_full_unstemmed | Modulation of microRNA expression in human lung cancer cells by the G9a histone methyltransferase inhibitor BIX01294 |
title_short | Modulation of microRNA expression in human lung cancer cells by the G9a histone methyltransferase inhibitor BIX01294 |
title_sort | modulation of microrna expression in human lung cancer cells by the g9a histone methyltransferase inhibitor bix01294 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049738/ https://www.ncbi.nlm.nih.gov/pubmed/24932239 http://dx.doi.org/10.3892/ol.2014.2034 |
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