Secondary Ion Mass Spectrometry Imaging of Dictyostelium discoideum Aggregation Streams

High resolution imaging mass spectrometry could become a valuable tool for cell and developmental biology, but both, high spatial and mass spectral resolution are needed to enable this. In this report, we employed Bi(3) bombardment time-of-flight (Bi(3) ToF-SIMS) and C(60) bombardment Fourier transf...

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Detalles Bibliográficos
Autores principales: DeBord, John Daniel, Smith, Donald F., Anderton, Christopher R., Heeren, Ron M. A., Paša-Tolić, Ljiljana, Gomer, Richard H., Fernandez-Lima, Francisco A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049834/
https://www.ncbi.nlm.nih.gov/pubmed/24911189
http://dx.doi.org/10.1371/journal.pone.0099319
Descripción
Sumario:High resolution imaging mass spectrometry could become a valuable tool for cell and developmental biology, but both, high spatial and mass spectral resolution are needed to enable this. In this report, we employed Bi(3) bombardment time-of-flight (Bi(3) ToF-SIMS) and C(60) bombardment Fourier transform ion cyclotron resonance secondary ion mass spectrometry (C(60) FTICR-SIMS) to image Dictyostelium discoideum aggregation streams. Nearly 300 lipid species were identified from the aggregation streams. High resolution mass spectrometry imaging (FTICR-SIMS) enabled the generation of multiple molecular ion maps at the nominal mass level and provided good coverage for fatty acyls, prenol lipids, and sterol lipids. The comparison of Bi(3) ToF-SIMS and C(60) FTICR-SIMS suggested that while the first provides fast, high spatial resolution molecular ion images, the chemical complexity of biological samples warrants the use of high resolution analyzers for accurate ion identification.

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