Cargando…

CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells

The germinal center (GC) is a dynamic microenvironment where antigen (Ag)-activated B cells rapidly expand and differentiate, generating plasma cells (PC) that produce high-affinity antibodies. Precise regulation of survival and proliferation of Ag-activated B cells within the GC is crucial for humo...

Descripción completa

Detalles Bibliográficos
Autores principales: Yoon, Sun-Ok, Lee, In Yong, Zhang, Xin, Zapata, Mariana C., Choi, Yong Sung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050195/
https://www.ncbi.nlm.nih.gov/pubmed/24918051
http://dx.doi.org/10.1016/j.fob.2014.04.001
_version_ 1782319916790054912
author Yoon, Sun-Ok
Lee, In Yong
Zhang, Xin
Zapata, Mariana C.
Choi, Yong Sung
author_facet Yoon, Sun-Ok
Lee, In Yong
Zhang, Xin
Zapata, Mariana C.
Choi, Yong Sung
author_sort Yoon, Sun-Ok
collection PubMed
description The germinal center (GC) is a dynamic microenvironment where antigen (Ag)-activated B cells rapidly expand and differentiate, generating plasma cells (PC) that produce high-affinity antibodies. Precise regulation of survival and proliferation of Ag-activated B cells within the GC is crucial for humoral immune responses. The follicular dendritic cells (FDC) are the specialized stromal cells in the GC that prevent apoptosis of GC-B cells. Recently, we reported that human GC-B cells consist of CD9+ and CD9− populations and that it is the CD9+ cells that are committed to the PC lineage. In this study, we investigated the functional role of CD9 on GC-B cells. Tonsillar tissue section staining revealed that in vivo CD9+ GC-B cells localized in the light zone FDC area. Consistent this, in vitro CD9+ GC-B cells survived better than CD9− GC-B cells in the presence of HK cells, an FDC line, in a cell–cell contact-dependent manner. The frozen tonsillar tissue section binding assay showed that CD9+ GC-B cells bound to the GC area of tonsillar tissues significantly more than the CD9− GC-B cells did and that the binding was significantly inhibited by neutralizing anti-integrin β1 antibody. Furthermore, CD9+ cells bound to soluble VCAM-1 more than CD9− cells did, resulting in activation and stabilization of the active epitope of integrin β1. All together, our data suggest that CD9 on GC-B cells contributes to survival by strengthening their binding to FDC through the VLA4/VCAM-1 axis.
format Online
Article
Text
id pubmed-4050195
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-40501952014-06-10 CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells Yoon, Sun-Ok Lee, In Yong Zhang, Xin Zapata, Mariana C. Choi, Yong Sung FEBS Open Bio Article The germinal center (GC) is a dynamic microenvironment where antigen (Ag)-activated B cells rapidly expand and differentiate, generating plasma cells (PC) that produce high-affinity antibodies. Precise regulation of survival and proliferation of Ag-activated B cells within the GC is crucial for humoral immune responses. The follicular dendritic cells (FDC) are the specialized stromal cells in the GC that prevent apoptosis of GC-B cells. Recently, we reported that human GC-B cells consist of CD9+ and CD9− populations and that it is the CD9+ cells that are committed to the PC lineage. In this study, we investigated the functional role of CD9 on GC-B cells. Tonsillar tissue section staining revealed that in vivo CD9+ GC-B cells localized in the light zone FDC area. Consistent this, in vitro CD9+ GC-B cells survived better than CD9− GC-B cells in the presence of HK cells, an FDC line, in a cell–cell contact-dependent manner. The frozen tonsillar tissue section binding assay showed that CD9+ GC-B cells bound to the GC area of tonsillar tissues significantly more than the CD9− GC-B cells did and that the binding was significantly inhibited by neutralizing anti-integrin β1 antibody. Furthermore, CD9+ cells bound to soluble VCAM-1 more than CD9− cells did, resulting in activation and stabilization of the active epitope of integrin β1. All together, our data suggest that CD9 on GC-B cells contributes to survival by strengthening their binding to FDC through the VLA4/VCAM-1 axis. Elsevier 2014-04-13 /pmc/articles/PMC4050195/ /pubmed/24918051 http://dx.doi.org/10.1016/j.fob.2014.04.001 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Yoon, Sun-Ok
Lee, In Yong
Zhang, Xin
Zapata, Mariana C.
Choi, Yong Sung
CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells
title CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells
title_full CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells
title_fullStr CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells
title_full_unstemmed CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells
title_short CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells
title_sort cd9 may contribute to the survival of human germinal center b cells by facilitating the interaction with follicular dendritic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050195/
https://www.ncbi.nlm.nih.gov/pubmed/24918051
http://dx.doi.org/10.1016/j.fob.2014.04.001
work_keys_str_mv AT yoonsunok cd9maycontributetothesurvivalofhumangerminalcenterbcellsbyfacilitatingtheinteractionwithfolliculardendriticcells
AT leeinyong cd9maycontributetothesurvivalofhumangerminalcenterbcellsbyfacilitatingtheinteractionwithfolliculardendriticcells
AT zhangxin cd9maycontributetothesurvivalofhumangerminalcenterbcellsbyfacilitatingtheinteractionwithfolliculardendriticcells
AT zapatamarianac cd9maycontributetothesurvivalofhumangerminalcenterbcellsbyfacilitatingtheinteractionwithfolliculardendriticcells
AT choiyongsung cd9maycontributetothesurvivalofhumangerminalcenterbcellsbyfacilitatingtheinteractionwithfolliculardendriticcells