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CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells
The germinal center (GC) is a dynamic microenvironment where antigen (Ag)-activated B cells rapidly expand and differentiate, generating plasma cells (PC) that produce high-affinity antibodies. Precise regulation of survival and proliferation of Ag-activated B cells within the GC is crucial for humo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050195/ https://www.ncbi.nlm.nih.gov/pubmed/24918051 http://dx.doi.org/10.1016/j.fob.2014.04.001 |
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author | Yoon, Sun-Ok Lee, In Yong Zhang, Xin Zapata, Mariana C. Choi, Yong Sung |
author_facet | Yoon, Sun-Ok Lee, In Yong Zhang, Xin Zapata, Mariana C. Choi, Yong Sung |
author_sort | Yoon, Sun-Ok |
collection | PubMed |
description | The germinal center (GC) is a dynamic microenvironment where antigen (Ag)-activated B cells rapidly expand and differentiate, generating plasma cells (PC) that produce high-affinity antibodies. Precise regulation of survival and proliferation of Ag-activated B cells within the GC is crucial for humoral immune responses. The follicular dendritic cells (FDC) are the specialized stromal cells in the GC that prevent apoptosis of GC-B cells. Recently, we reported that human GC-B cells consist of CD9+ and CD9− populations and that it is the CD9+ cells that are committed to the PC lineage. In this study, we investigated the functional role of CD9 on GC-B cells. Tonsillar tissue section staining revealed that in vivo CD9+ GC-B cells localized in the light zone FDC area. Consistent this, in vitro CD9+ GC-B cells survived better than CD9− GC-B cells in the presence of HK cells, an FDC line, in a cell–cell contact-dependent manner. The frozen tonsillar tissue section binding assay showed that CD9+ GC-B cells bound to the GC area of tonsillar tissues significantly more than the CD9− GC-B cells did and that the binding was significantly inhibited by neutralizing anti-integrin β1 antibody. Furthermore, CD9+ cells bound to soluble VCAM-1 more than CD9− cells did, resulting in activation and stabilization of the active epitope of integrin β1. All together, our data suggest that CD9 on GC-B cells contributes to survival by strengthening their binding to FDC through the VLA4/VCAM-1 axis. |
format | Online Article Text |
id | pubmed-4050195 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-40501952014-06-10 CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells Yoon, Sun-Ok Lee, In Yong Zhang, Xin Zapata, Mariana C. Choi, Yong Sung FEBS Open Bio Article The germinal center (GC) is a dynamic microenvironment where antigen (Ag)-activated B cells rapidly expand and differentiate, generating plasma cells (PC) that produce high-affinity antibodies. Precise regulation of survival and proliferation of Ag-activated B cells within the GC is crucial for humoral immune responses. The follicular dendritic cells (FDC) are the specialized stromal cells in the GC that prevent apoptosis of GC-B cells. Recently, we reported that human GC-B cells consist of CD9+ and CD9− populations and that it is the CD9+ cells that are committed to the PC lineage. In this study, we investigated the functional role of CD9 on GC-B cells. Tonsillar tissue section staining revealed that in vivo CD9+ GC-B cells localized in the light zone FDC area. Consistent this, in vitro CD9+ GC-B cells survived better than CD9− GC-B cells in the presence of HK cells, an FDC line, in a cell–cell contact-dependent manner. The frozen tonsillar tissue section binding assay showed that CD9+ GC-B cells bound to the GC area of tonsillar tissues significantly more than the CD9− GC-B cells did and that the binding was significantly inhibited by neutralizing anti-integrin β1 antibody. Furthermore, CD9+ cells bound to soluble VCAM-1 more than CD9− cells did, resulting in activation and stabilization of the active epitope of integrin β1. All together, our data suggest that CD9 on GC-B cells contributes to survival by strengthening their binding to FDC through the VLA4/VCAM-1 axis. Elsevier 2014-04-13 /pmc/articles/PMC4050195/ /pubmed/24918051 http://dx.doi.org/10.1016/j.fob.2014.04.001 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Article Yoon, Sun-Ok Lee, In Yong Zhang, Xin Zapata, Mariana C. Choi, Yong Sung CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells |
title | CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells |
title_full | CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells |
title_fullStr | CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells |
title_full_unstemmed | CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells |
title_short | CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells |
title_sort | cd9 may contribute to the survival of human germinal center b cells by facilitating the interaction with follicular dendritic cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050195/ https://www.ncbi.nlm.nih.gov/pubmed/24918051 http://dx.doi.org/10.1016/j.fob.2014.04.001 |
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