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3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

Cornification and epidermal barrier defects are associated with a number of clinically diverse skin disorders. However, a suitable in vitro model for studying normal barrier function and barrier defects is still lacking. Here, we demonstrate the generation of human epidermal equivalents (HEEs) from...

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Autores principales: Petrova, Anastasia, Celli, Anna, Jacquet, Laureen, Dafou, Dimitra, Crumrine, Debra, Hupe, Melanie, Arno, Matthew, Hobbs, Carl, Cvoro, Aleksandra, Karagiannis, Panagiotis, Devito, Liani, Sun, Richard, Adame, Lillian C., Vaughan, Robert, McGrath, John A., Mauro, Theodora M., Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050479/
https://www.ncbi.nlm.nih.gov/pubmed/24936454
http://dx.doi.org/10.1016/j.stemcr.2014.03.009
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author Petrova, Anastasia
Celli, Anna
Jacquet, Laureen
Dafou, Dimitra
Crumrine, Debra
Hupe, Melanie
Arno, Matthew
Hobbs, Carl
Cvoro, Aleksandra
Karagiannis, Panagiotis
Devito, Liani
Sun, Richard
Adame, Lillian C.
Vaughan, Robert
McGrath, John A.
Mauro, Theodora M.
Ilic, Dusko
author_facet Petrova, Anastasia
Celli, Anna
Jacquet, Laureen
Dafou, Dimitra
Crumrine, Debra
Hupe, Melanie
Arno, Matthew
Hobbs, Carl
Cvoro, Aleksandra
Karagiannis, Panagiotis
Devito, Liani
Sun, Richard
Adame, Lillian C.
Vaughan, Robert
McGrath, John A.
Mauro, Theodora M.
Ilic, Dusko
author_sort Petrova, Anastasia
collection PubMed
description Cornification and epidermal barrier defects are associated with a number of clinically diverse skin disorders. However, a suitable in vitro model for studying normal barrier function and barrier defects is still lacking. Here, we demonstrate the generation of human epidermal equivalents (HEEs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). HEEs are structurally similar to native epidermis, with a functional permeability barrier. We exposed a pure population of hESC/iPSC-derived keratinocytes, whose transcriptome corresponds to the gene signature of normal primary human keratinocytes (NHKs), to a sequential high-to-low humidity environment in an air/liquid interface culture. The resulting HEEs had all of the cellular strata of the human epidermis, with skin barrier properties similar to those of normal skin. Such HEEs generated from disease-specific iPSCs will be an invaluable tool not only for dissecting molecular mechanisms that lead to epidermal barrier defects but also for drug development and screening.
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spelling pubmed-40504792014-06-16 3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Petrova, Anastasia Celli, Anna Jacquet, Laureen Dafou, Dimitra Crumrine, Debra Hupe, Melanie Arno, Matthew Hobbs, Carl Cvoro, Aleksandra Karagiannis, Panagiotis Devito, Liani Sun, Richard Adame, Lillian C. Vaughan, Robert McGrath, John A. Mauro, Theodora M. Ilic, Dusko Stem Cell Reports Article Cornification and epidermal barrier defects are associated with a number of clinically diverse skin disorders. However, a suitable in vitro model for studying normal barrier function and barrier defects is still lacking. Here, we demonstrate the generation of human epidermal equivalents (HEEs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). HEEs are structurally similar to native epidermis, with a functional permeability barrier. We exposed a pure population of hESC/iPSC-derived keratinocytes, whose transcriptome corresponds to the gene signature of normal primary human keratinocytes (NHKs), to a sequential high-to-low humidity environment in an air/liquid interface culture. The resulting HEEs had all of the cellular strata of the human epidermis, with skin barrier properties similar to those of normal skin. Such HEEs generated from disease-specific iPSCs will be an invaluable tool not only for dissecting molecular mechanisms that lead to epidermal barrier defects but also for drug development and screening. Elsevier 2014-04-24 /pmc/articles/PMC4050479/ /pubmed/24936454 http://dx.doi.org/10.1016/j.stemcr.2014.03.009 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Petrova, Anastasia
Celli, Anna
Jacquet, Laureen
Dafou, Dimitra
Crumrine, Debra
Hupe, Melanie
Arno, Matthew
Hobbs, Carl
Cvoro, Aleksandra
Karagiannis, Panagiotis
Devito, Liani
Sun, Richard
Adame, Lillian C.
Vaughan, Robert
McGrath, John A.
Mauro, Theodora M.
Ilic, Dusko
3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
title 3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
title_full 3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
title_fullStr 3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
title_full_unstemmed 3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
title_short 3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
title_sort 3d in vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050479/
https://www.ncbi.nlm.nih.gov/pubmed/24936454
http://dx.doi.org/10.1016/j.stemcr.2014.03.009
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