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Injury to the Spinal Cord Niche Alters the Engraftment Dynamics of Human Neural Stem Cells
The microenvironment is a critical mediator of stem cell survival, proliferation, migration, and differentiation. The majority of preclinical studies involving transplantation of neural stem cells (NSCs) into the CNS have focused on injured or degenerating microenvironments, leaving a dearth of info...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050489/ https://www.ncbi.nlm.nih.gov/pubmed/24936450 http://dx.doi.org/10.1016/j.stemcr.2014.03.005 |
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author | Sontag, Christopher J. Uchida, Nobuko Cummings, Brian J. Anderson, Aileen J. |
author_facet | Sontag, Christopher J. Uchida, Nobuko Cummings, Brian J. Anderson, Aileen J. |
author_sort | Sontag, Christopher J. |
collection | PubMed |
description | The microenvironment is a critical mediator of stem cell survival, proliferation, migration, and differentiation. The majority of preclinical studies involving transplantation of neural stem cells (NSCs) into the CNS have focused on injured or degenerating microenvironments, leaving a dearth of information as to how NSCs differentially respond to intact versus damaged CNS. Furthermore, single, terminal histological endpoints predominate, providing limited insight into the spatiotemporal dynamics of NSC engraftment and migration. We investigated the early and long-term engraftment dynamics of human CNS stem cells propagated as neurospheres (hCNS-SCns) following transplantation into uninjured versus subacutely injured spinal cords of immunodeficient NOD-scid mice. We stereologically quantified engraftment, survival, proliferation, migration, and differentiation at 1, 7, 14, 28, and 98 days posttransplantation, and identified injury-dependent alterations. Notably, the injured microenvironment decreased hCNS-SCns survival, delayed and altered the location of proliferation, influenced both total and fate-specific migration, and promoted oligodendrocyte maturation. |
format | Online Article Text |
id | pubmed-4050489 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-40504892014-06-16 Injury to the Spinal Cord Niche Alters the Engraftment Dynamics of Human Neural Stem Cells Sontag, Christopher J. Uchida, Nobuko Cummings, Brian J. Anderson, Aileen J. Stem Cell Reports Article The microenvironment is a critical mediator of stem cell survival, proliferation, migration, and differentiation. The majority of preclinical studies involving transplantation of neural stem cells (NSCs) into the CNS have focused on injured or degenerating microenvironments, leaving a dearth of information as to how NSCs differentially respond to intact versus damaged CNS. Furthermore, single, terminal histological endpoints predominate, providing limited insight into the spatiotemporal dynamics of NSC engraftment and migration. We investigated the early and long-term engraftment dynamics of human CNS stem cells propagated as neurospheres (hCNS-SCns) following transplantation into uninjured versus subacutely injured spinal cords of immunodeficient NOD-scid mice. We stereologically quantified engraftment, survival, proliferation, migration, and differentiation at 1, 7, 14, 28, and 98 days posttransplantation, and identified injury-dependent alterations. Notably, the injured microenvironment decreased hCNS-SCns survival, delayed and altered the location of proliferation, influenced both total and fate-specific migration, and promoted oligodendrocyte maturation. Elsevier 2014-04-18 /pmc/articles/PMC4050489/ /pubmed/24936450 http://dx.doi.org/10.1016/j.stemcr.2014.03.005 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Article Sontag, Christopher J. Uchida, Nobuko Cummings, Brian J. Anderson, Aileen J. Injury to the Spinal Cord Niche Alters the Engraftment Dynamics of Human Neural Stem Cells |
title | Injury to the Spinal Cord Niche Alters the Engraftment Dynamics of Human Neural Stem Cells |
title_full | Injury to the Spinal Cord Niche Alters the Engraftment Dynamics of Human Neural Stem Cells |
title_fullStr | Injury to the Spinal Cord Niche Alters the Engraftment Dynamics of Human Neural Stem Cells |
title_full_unstemmed | Injury to the Spinal Cord Niche Alters the Engraftment Dynamics of Human Neural Stem Cells |
title_short | Injury to the Spinal Cord Niche Alters the Engraftment Dynamics of Human Neural Stem Cells |
title_sort | injury to the spinal cord niche alters the engraftment dynamics of human neural stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050489/ https://www.ncbi.nlm.nih.gov/pubmed/24936450 http://dx.doi.org/10.1016/j.stemcr.2014.03.005 |
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