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Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns
In vitro models of Chlamydia trachomatis growth have long been studied to predict growth in vivo. Alternative or persistent growth modes in vitro have been shown to occur under the influence of numerous stressors but have not been studied in vivo. Here, we report the development of methods for sampl...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050528/ https://www.ncbi.nlm.nih.gov/pubmed/24959423 http://dx.doi.org/10.3389/fcimb.2014.00071 |
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author | Lewis, Maria E. Belland, Robert J. AbdelRahman, Yasser M. Beatty, Wandy L. Aiyar, Ashok A. Zea, Arnold H. Greene, Sheila J. Marrero, Luis Buckner, Lyndsey R. Tate, David J. McGowin, Chris L. Kozlowski, Pamela A. O'Brien, Michelle Lillis, Rebecca A. Martin, David H. Quayle, Alison J. |
author_facet | Lewis, Maria E. Belland, Robert J. AbdelRahman, Yasser M. Beatty, Wandy L. Aiyar, Ashok A. Zea, Arnold H. Greene, Sheila J. Marrero, Luis Buckner, Lyndsey R. Tate, David J. McGowin, Chris L. Kozlowski, Pamela A. O'Brien, Michelle Lillis, Rebecca A. Martin, David H. Quayle, Alison J. |
author_sort | Lewis, Maria E. |
collection | PubMed |
description | In vitro models of Chlamydia trachomatis growth have long been studied to predict growth in vivo. Alternative or persistent growth modes in vitro have been shown to occur under the influence of numerous stressors but have not been studied in vivo. Here, we report the development of methods for sampling human infections from the endocervix in a manner that permits a multifaceted analysis of the bacteria, host and the endocervical environment. Our approach permits evaluating total bacterial load, transcriptional patterns, morphology by immunofluorescence and electron microscopy, and levels of cytokines and nutrients in the infection microenvironment. By applying this approach to two pilot patients with disparate infections, we have determined that their contrasting growth patterns correlate with strikingly distinct transcriptional biomarkers, and are associated with differences in local levels of IFNγ. Our multifaceted approach will be useful to dissect infections in the human host and be useful in identifying patients at risk for chronic disease. Importantly, the molecular and morphological analyses described here indicate that persistent growth forms can be isolated from the human endocervix when the infection microenvironment resembles the in vitro model of IFNγ-induced persistence. |
format | Online Article Text |
id | pubmed-4050528 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-40505282014-06-23 Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns Lewis, Maria E. Belland, Robert J. AbdelRahman, Yasser M. Beatty, Wandy L. Aiyar, Ashok A. Zea, Arnold H. Greene, Sheila J. Marrero, Luis Buckner, Lyndsey R. Tate, David J. McGowin, Chris L. Kozlowski, Pamela A. O'Brien, Michelle Lillis, Rebecca A. Martin, David H. Quayle, Alison J. Front Cell Infect Microbiol Microbiology In vitro models of Chlamydia trachomatis growth have long been studied to predict growth in vivo. Alternative or persistent growth modes in vitro have been shown to occur under the influence of numerous stressors but have not been studied in vivo. Here, we report the development of methods for sampling human infections from the endocervix in a manner that permits a multifaceted analysis of the bacteria, host and the endocervical environment. Our approach permits evaluating total bacterial load, transcriptional patterns, morphology by immunofluorescence and electron microscopy, and levels of cytokines and nutrients in the infection microenvironment. By applying this approach to two pilot patients with disparate infections, we have determined that their contrasting growth patterns correlate with strikingly distinct transcriptional biomarkers, and are associated with differences in local levels of IFNγ. Our multifaceted approach will be useful to dissect infections in the human host and be useful in identifying patients at risk for chronic disease. Importantly, the molecular and morphological analyses described here indicate that persistent growth forms can be isolated from the human endocervix when the infection microenvironment resembles the in vitro model of IFNγ-induced persistence. Frontiers Media S.A. 2014-06-10 /pmc/articles/PMC4050528/ /pubmed/24959423 http://dx.doi.org/10.3389/fcimb.2014.00071 Text en Copyright © 2014 Lewis, Belland, AbdelRahman, Beatty, Aiyar, Zea, Greene, Marrero, Buckner, Tate, McGowin, Kozlowski, O'Brien, Lillis, Martin and Quayle. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Lewis, Maria E. Belland, Robert J. AbdelRahman, Yasser M. Beatty, Wandy L. Aiyar, Ashok A. Zea, Arnold H. Greene, Sheila J. Marrero, Luis Buckner, Lyndsey R. Tate, David J. McGowin, Chris L. Kozlowski, Pamela A. O'Brien, Michelle Lillis, Rebecca A. Martin, David H. Quayle, Alison J. Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns |
title | Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns |
title_full | Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns |
title_fullStr | Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns |
title_full_unstemmed | Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns |
title_short | Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns |
title_sort | morphologic and molecular evaluation of chlamydia trachomatis growth in human endocervix reveals distinct growth patterns |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050528/ https://www.ncbi.nlm.nih.gov/pubmed/24959423 http://dx.doi.org/10.3389/fcimb.2014.00071 |
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