Cargando…

Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns

In vitro models of Chlamydia trachomatis growth have long been studied to predict growth in vivo. Alternative or persistent growth modes in vitro have been shown to occur under the influence of numerous stressors but have not been studied in vivo. Here, we report the development of methods for sampl...

Descripción completa

Detalles Bibliográficos
Autores principales: Lewis, Maria E., Belland, Robert J., AbdelRahman, Yasser M., Beatty, Wandy L., Aiyar, Ashok A., Zea, Arnold H., Greene, Sheila J., Marrero, Luis, Buckner, Lyndsey R., Tate, David J., McGowin, Chris L., Kozlowski, Pamela A., O'Brien, Michelle, Lillis, Rebecca A., Martin, David H., Quayle, Alison J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050528/
https://www.ncbi.nlm.nih.gov/pubmed/24959423
http://dx.doi.org/10.3389/fcimb.2014.00071
_version_ 1782319969756774400
author Lewis, Maria E.
Belland, Robert J.
AbdelRahman, Yasser M.
Beatty, Wandy L.
Aiyar, Ashok A.
Zea, Arnold H.
Greene, Sheila J.
Marrero, Luis
Buckner, Lyndsey R.
Tate, David J.
McGowin, Chris L.
Kozlowski, Pamela A.
O'Brien, Michelle
Lillis, Rebecca A.
Martin, David H.
Quayle, Alison J.
author_facet Lewis, Maria E.
Belland, Robert J.
AbdelRahman, Yasser M.
Beatty, Wandy L.
Aiyar, Ashok A.
Zea, Arnold H.
Greene, Sheila J.
Marrero, Luis
Buckner, Lyndsey R.
Tate, David J.
McGowin, Chris L.
Kozlowski, Pamela A.
O'Brien, Michelle
Lillis, Rebecca A.
Martin, David H.
Quayle, Alison J.
author_sort Lewis, Maria E.
collection PubMed
description In vitro models of Chlamydia trachomatis growth have long been studied to predict growth in vivo. Alternative or persistent growth modes in vitro have been shown to occur under the influence of numerous stressors but have not been studied in vivo. Here, we report the development of methods for sampling human infections from the endocervix in a manner that permits a multifaceted analysis of the bacteria, host and the endocervical environment. Our approach permits evaluating total bacterial load, transcriptional patterns, morphology by immunofluorescence and electron microscopy, and levels of cytokines and nutrients in the infection microenvironment. By applying this approach to two pilot patients with disparate infections, we have determined that their contrasting growth patterns correlate with strikingly distinct transcriptional biomarkers, and are associated with differences in local levels of IFNγ. Our multifaceted approach will be useful to dissect infections in the human host and be useful in identifying patients at risk for chronic disease. Importantly, the molecular and morphological analyses described here indicate that persistent growth forms can be isolated from the human endocervix when the infection microenvironment resembles the in vitro model of IFNγ-induced persistence.
format Online
Article
Text
id pubmed-4050528
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-40505282014-06-23 Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns Lewis, Maria E. Belland, Robert J. AbdelRahman, Yasser M. Beatty, Wandy L. Aiyar, Ashok A. Zea, Arnold H. Greene, Sheila J. Marrero, Luis Buckner, Lyndsey R. Tate, David J. McGowin, Chris L. Kozlowski, Pamela A. O'Brien, Michelle Lillis, Rebecca A. Martin, David H. Quayle, Alison J. Front Cell Infect Microbiol Microbiology In vitro models of Chlamydia trachomatis growth have long been studied to predict growth in vivo. Alternative or persistent growth modes in vitro have been shown to occur under the influence of numerous stressors but have not been studied in vivo. Here, we report the development of methods for sampling human infections from the endocervix in a manner that permits a multifaceted analysis of the bacteria, host and the endocervical environment. Our approach permits evaluating total bacterial load, transcriptional patterns, morphology by immunofluorescence and electron microscopy, and levels of cytokines and nutrients in the infection microenvironment. By applying this approach to two pilot patients with disparate infections, we have determined that their contrasting growth patterns correlate with strikingly distinct transcriptional biomarkers, and are associated with differences in local levels of IFNγ. Our multifaceted approach will be useful to dissect infections in the human host and be useful in identifying patients at risk for chronic disease. Importantly, the molecular and morphological analyses described here indicate that persistent growth forms can be isolated from the human endocervix when the infection microenvironment resembles the in vitro model of IFNγ-induced persistence. Frontiers Media S.A. 2014-06-10 /pmc/articles/PMC4050528/ /pubmed/24959423 http://dx.doi.org/10.3389/fcimb.2014.00071 Text en Copyright © 2014 Lewis, Belland, AbdelRahman, Beatty, Aiyar, Zea, Greene, Marrero, Buckner, Tate, McGowin, Kozlowski, O'Brien, Lillis, Martin and Quayle. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lewis, Maria E.
Belland, Robert J.
AbdelRahman, Yasser M.
Beatty, Wandy L.
Aiyar, Ashok A.
Zea, Arnold H.
Greene, Sheila J.
Marrero, Luis
Buckner, Lyndsey R.
Tate, David J.
McGowin, Chris L.
Kozlowski, Pamela A.
O'Brien, Michelle
Lillis, Rebecca A.
Martin, David H.
Quayle, Alison J.
Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns
title Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns
title_full Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns
title_fullStr Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns
title_full_unstemmed Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns
title_short Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns
title_sort morphologic and molecular evaluation of chlamydia trachomatis growth in human endocervix reveals distinct growth patterns
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050528/
https://www.ncbi.nlm.nih.gov/pubmed/24959423
http://dx.doi.org/10.3389/fcimb.2014.00071
work_keys_str_mv AT lewismariae morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT bellandrobertj morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT abdelrahmanyasserm morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT beattywandyl morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT aiyarashoka morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT zeaarnoldh morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT greenesheilaj morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT marreroluis morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT bucknerlyndseyr morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT tatedavidj morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT mcgowinchrisl morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT kozlowskipamelaa morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT obrienmichelle morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT lillisrebeccaa morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT martindavidh morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns
AT quaylealisonj morphologicandmolecularevaluationofchlamydiatrachomatisgrowthinhumanendocervixrevealsdistinctgrowthpatterns