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Detection of piroplasms infection in sheep, dogs and hedgehogs in Central China
BACKGROUND: Piroplasms are kinds of tick-borne parasitic apicomplexan protozoa, which are detrimental to humans and animals in tropical and subtropical areas around the world. Up until now, there has been a limited amount of reliable information available about the prevalence of piroplasms infection...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051148/ https://www.ncbi.nlm.nih.gov/pubmed/24917932 http://dx.doi.org/10.1186/2049-9957-3-18 |
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author | Chen, Zhuo Liu, Qin Jiao, Feng-Chao Xu, Bian-Li Zhou, Xiao-Nong |
author_facet | Chen, Zhuo Liu, Qin Jiao, Feng-Chao Xu, Bian-Li Zhou, Xiao-Nong |
author_sort | Chen, Zhuo |
collection | PubMed |
description | BACKGROUND: Piroplasms are kinds of tick-borne parasitic apicomplexan protozoa, which are detrimental to humans and animals in tropical and subtropical areas around the world. Up until now, there has been a limited amount of reliable information available about the prevalence of piroplasms infections in wild animals in China. Therefore, we have investigated the infections of Babesia and Theileria species in both domestic and wild animals in Xinyang city, Henan province, where tick-borne diseases have recently been reported. This study aims to analyze the distribution patterns of piroplasms infections in animals, and assess their potential threat to humans in Central China. METHODS: Blood samples were collected from sheep, dogs and hedgehogs in two regions, including Shihe District and Luoshan County, of Xinyang city, Henan province from August to December 2012. Babesia spp. and Theileria spp. were detected by polymerase chain reaction (PCR) and identified by sequencing and phylogenetic analysis. Moreover, the characteristics of detected piroplasms in different animal hosts were compared between the two study regions. RESULTS: A total of 227 blood samples were collected from 73 sheep, two dogs and 152 hedgehogs. Babesia spp. was only detected in the two dogs. Theileria spp. was detected both in the sheep and the hedgehogs, and the total positive rate of Theileria spp. in the sheep and the hedgehogs was 57.53% and 13.82%, respectively. Sequencing and phylogenetic analysis revealed that the Theileria spp. detected in the sheep and the hedgehogs were very close to T. lunwenshuni cloned from a small ruminant and Theileria spp. isolated from a febrile hospitalized patient in China. CONCLUSION: Babesia and Theileria infections were detected in both domestic and wild animals in Xinyang city, Henan province in Central China, thus warranting further studies in these regions. |
format | Online Article Text |
id | pubmed-4051148 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40511482014-06-11 Detection of piroplasms infection in sheep, dogs and hedgehogs in Central China Chen, Zhuo Liu, Qin Jiao, Feng-Chao Xu, Bian-Li Zhou, Xiao-Nong Infect Dis Poverty Research Article BACKGROUND: Piroplasms are kinds of tick-borne parasitic apicomplexan protozoa, which are detrimental to humans and animals in tropical and subtropical areas around the world. Up until now, there has been a limited amount of reliable information available about the prevalence of piroplasms infections in wild animals in China. Therefore, we have investigated the infections of Babesia and Theileria species in both domestic and wild animals in Xinyang city, Henan province, where tick-borne diseases have recently been reported. This study aims to analyze the distribution patterns of piroplasms infections in animals, and assess their potential threat to humans in Central China. METHODS: Blood samples were collected from sheep, dogs and hedgehogs in two regions, including Shihe District and Luoshan County, of Xinyang city, Henan province from August to December 2012. Babesia spp. and Theileria spp. were detected by polymerase chain reaction (PCR) and identified by sequencing and phylogenetic analysis. Moreover, the characteristics of detected piroplasms in different animal hosts were compared between the two study regions. RESULTS: A total of 227 blood samples were collected from 73 sheep, two dogs and 152 hedgehogs. Babesia spp. was only detected in the two dogs. Theileria spp. was detected both in the sheep and the hedgehogs, and the total positive rate of Theileria spp. in the sheep and the hedgehogs was 57.53% and 13.82%, respectively. Sequencing and phylogenetic analysis revealed that the Theileria spp. detected in the sheep and the hedgehogs were very close to T. lunwenshuni cloned from a small ruminant and Theileria spp. isolated from a febrile hospitalized patient in China. CONCLUSION: Babesia and Theileria infections were detected in both domestic and wild animals in Xinyang city, Henan province in Central China, thus warranting further studies in these regions. BioMed Central 2014-06-03 /pmc/articles/PMC4051148/ /pubmed/24917932 http://dx.doi.org/10.1186/2049-9957-3-18 Text en Copyright © 2014 Chen et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Chen, Zhuo Liu, Qin Jiao, Feng-Chao Xu, Bian-Li Zhou, Xiao-Nong Detection of piroplasms infection in sheep, dogs and hedgehogs in Central China |
title | Detection of piroplasms infection in sheep, dogs and hedgehogs in Central China |
title_full | Detection of piroplasms infection in sheep, dogs and hedgehogs in Central China |
title_fullStr | Detection of piroplasms infection in sheep, dogs and hedgehogs in Central China |
title_full_unstemmed | Detection of piroplasms infection in sheep, dogs and hedgehogs in Central China |
title_short | Detection of piroplasms infection in sheep, dogs and hedgehogs in Central China |
title_sort | detection of piroplasms infection in sheep, dogs and hedgehogs in central china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051148/ https://www.ncbi.nlm.nih.gov/pubmed/24917932 http://dx.doi.org/10.1186/2049-9957-3-18 |
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