Cargando…

Expression and Regulation of the Proton-Coupled Oligopeptide Transporter PhT2 by LPS in Macrophages and Mouse Spleen

[Image: see text] Membrane transporter PhT2 (SLC15A3), which belongs to the proton-coupled oligopeptide transporter family, mediates the transport of di/tripeptides and histidine utilizing an inwardly directed proton gradient and negative membrane potential. The aim of this study was to elucidate th...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yuqing, Sun, Dongli, Song, Feifeng, Hu, Yongjun, Smith, David E., Jiang, Huidi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051248/
https://www.ncbi.nlm.nih.gov/pubmed/24754256
http://dx.doi.org/10.1021/mp500014r
_version_ 1782320080626909184
author Wang, Yuqing
Sun, Dongli
Song, Feifeng
Hu, Yongjun
Smith, David E.
Jiang, Huidi
author_facet Wang, Yuqing
Sun, Dongli
Song, Feifeng
Hu, Yongjun
Smith, David E.
Jiang, Huidi
author_sort Wang, Yuqing
collection PubMed
description [Image: see text] Membrane transporter PhT2 (SLC15A3), which belongs to the proton-coupled oligopeptide transporter family, mediates the transport of di/tripeptides and histidine utilizing an inwardly directed proton gradient and negative membrane potential. The aim of this study was to elucidate the molecular expression of PhT2 in macrophages and mouse tissues and to explore the regulation of PhT2 by lipopolysaccharide (LPS). The results showed relatively high expression of PhT2 in J774A.1 and THP-1 macrophage cells, mouse spleen, and lung. Using an LPS-induced inflammatory cell model, we found that hPhT2 mRNA expression was up-regulated in THP-1 cells and that the up-regulation was suppressed by pyrrolidine dithiocarbamate, a specific inhibitor of NF-κB. Similar results were observed in mouse spleen during LPS-induced acute inflammation. Using dual-labeling immunofluorescence and confocal laser scanning microscopy, we confirmed that mPhT2 was colocalizing with lysosome-associated membrane protein 1 in transfected HEK293 cells. These results suggested that PhT2, a lysosomal membrane transporter, was up-regulated by LPS via the NF-κB signaling pathway.
format Online
Article
Text
id pubmed-4051248
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-40512482015-04-23 Expression and Regulation of the Proton-Coupled Oligopeptide Transporter PhT2 by LPS in Macrophages and Mouse Spleen Wang, Yuqing Sun, Dongli Song, Feifeng Hu, Yongjun Smith, David E. Jiang, Huidi Mol Pharm [Image: see text] Membrane transporter PhT2 (SLC15A3), which belongs to the proton-coupled oligopeptide transporter family, mediates the transport of di/tripeptides and histidine utilizing an inwardly directed proton gradient and negative membrane potential. The aim of this study was to elucidate the molecular expression of PhT2 in macrophages and mouse tissues and to explore the regulation of PhT2 by lipopolysaccharide (LPS). The results showed relatively high expression of PhT2 in J774A.1 and THP-1 macrophage cells, mouse spleen, and lung. Using an LPS-induced inflammatory cell model, we found that hPhT2 mRNA expression was up-regulated in THP-1 cells and that the up-regulation was suppressed by pyrrolidine dithiocarbamate, a specific inhibitor of NF-κB. Similar results were observed in mouse spleen during LPS-induced acute inflammation. Using dual-labeling immunofluorescence and confocal laser scanning microscopy, we confirmed that mPhT2 was colocalizing with lysosome-associated membrane protein 1 in transfected HEK293 cells. These results suggested that PhT2, a lysosomal membrane transporter, was up-regulated by LPS via the NF-κB signaling pathway. American Chemical Society 2014-04-23 2014-06-02 /pmc/articles/PMC4051248/ /pubmed/24754256 http://dx.doi.org/10.1021/mp500014r Text en Copyright © 2014 American Chemical Society
spellingShingle Wang, Yuqing
Sun, Dongli
Song, Feifeng
Hu, Yongjun
Smith, David E.
Jiang, Huidi
Expression and Regulation of the Proton-Coupled Oligopeptide Transporter PhT2 by LPS in Macrophages and Mouse Spleen
title Expression and Regulation of the Proton-Coupled Oligopeptide Transporter PhT2 by LPS in Macrophages and Mouse Spleen
title_full Expression and Regulation of the Proton-Coupled Oligopeptide Transporter PhT2 by LPS in Macrophages and Mouse Spleen
title_fullStr Expression and Regulation of the Proton-Coupled Oligopeptide Transporter PhT2 by LPS in Macrophages and Mouse Spleen
title_full_unstemmed Expression and Regulation of the Proton-Coupled Oligopeptide Transporter PhT2 by LPS in Macrophages and Mouse Spleen
title_short Expression and Regulation of the Proton-Coupled Oligopeptide Transporter PhT2 by LPS in Macrophages and Mouse Spleen
title_sort expression and regulation of the proton-coupled oligopeptide transporter pht2 by lps in macrophages and mouse spleen
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051248/
https://www.ncbi.nlm.nih.gov/pubmed/24754256
http://dx.doi.org/10.1021/mp500014r
work_keys_str_mv AT wangyuqing expressionandregulationoftheprotoncoupledoligopeptidetransporterpht2bylpsinmacrophagesandmousespleen
AT sundongli expressionandregulationoftheprotoncoupledoligopeptidetransporterpht2bylpsinmacrophagesandmousespleen
AT songfeifeng expressionandregulationoftheprotoncoupledoligopeptidetransporterpht2bylpsinmacrophagesandmousespleen
AT huyongjun expressionandregulationoftheprotoncoupledoligopeptidetransporterpht2bylpsinmacrophagesandmousespleen
AT smithdavide expressionandregulationoftheprotoncoupledoligopeptidetransporterpht2bylpsinmacrophagesandmousespleen
AT jianghuidi expressionandregulationoftheprotoncoupledoligopeptidetransporterpht2bylpsinmacrophagesandmousespleen