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Imaging of polysaccharides in the tomato cell wall with Raman microspectroscopy

BACKGROUND: The primary cell wall of fruits and vegetables is a structure mainly composed of polysaccharides (pectins, hemicelluloses, cellulose). Polysaccharides are assembled into a network and linked together. It is thought that the percentage of components and of plant cell wall has an important...

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Autores principales: Chylińska, Monika, Szymańska-Chargot, Monika, Zdunek, Artur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051370/
https://www.ncbi.nlm.nih.gov/pubmed/24917885
http://dx.doi.org/10.1186/1746-4811-10-14
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author Chylińska, Monika
Szymańska-Chargot, Monika
Zdunek, Artur
author_facet Chylińska, Monika
Szymańska-Chargot, Monika
Zdunek, Artur
author_sort Chylińska, Monika
collection PubMed
description BACKGROUND: The primary cell wall of fruits and vegetables is a structure mainly composed of polysaccharides (pectins, hemicelluloses, cellulose). Polysaccharides are assembled into a network and linked together. It is thought that the percentage of components and of plant cell wall has an important influence on mechanical properties of fruits and vegetables. RESULTS: In this study the Raman microspectroscopy technique was introduced to the visualization of the distribution of polysaccharides in cell wall of fruit. The methodology of the sample preparation, the measurement using Raman microscope and multivariate image analysis are discussed. Single band imaging (for preliminary analysis) and multivariate image analysis methods (principal component analysis and multivariate curve resolution) were used for the identification and localization of the components in the primary cell wall. CONCLUSIONS: Raman microspectroscopy supported by multivariate image analysis methods is useful in distinguishing cellulose and pectins in the cell wall in tomatoes. It presents how the localization of biopolymers was possible with minimally prepared samples.
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spelling pubmed-40513702014-06-11 Imaging of polysaccharides in the tomato cell wall with Raman microspectroscopy Chylińska, Monika Szymańska-Chargot, Monika Zdunek, Artur Plant Methods Methodology BACKGROUND: The primary cell wall of fruits and vegetables is a structure mainly composed of polysaccharides (pectins, hemicelluloses, cellulose). Polysaccharides are assembled into a network and linked together. It is thought that the percentage of components and of plant cell wall has an important influence on mechanical properties of fruits and vegetables. RESULTS: In this study the Raman microspectroscopy technique was introduced to the visualization of the distribution of polysaccharides in cell wall of fruit. The methodology of the sample preparation, the measurement using Raman microscope and multivariate image analysis are discussed. Single band imaging (for preliminary analysis) and multivariate image analysis methods (principal component analysis and multivariate curve resolution) were used for the identification and localization of the components in the primary cell wall. CONCLUSIONS: Raman microspectroscopy supported by multivariate image analysis methods is useful in distinguishing cellulose and pectins in the cell wall in tomatoes. It presents how the localization of biopolymers was possible with minimally prepared samples. BioMed Central 2014-05-29 /pmc/articles/PMC4051370/ /pubmed/24917885 http://dx.doi.org/10.1186/1746-4811-10-14 Text en Copyright © 2014 Chylińska et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology
Chylińska, Monika
Szymańska-Chargot, Monika
Zdunek, Artur
Imaging of polysaccharides in the tomato cell wall with Raman microspectroscopy
title Imaging of polysaccharides in the tomato cell wall with Raman microspectroscopy
title_full Imaging of polysaccharides in the tomato cell wall with Raman microspectroscopy
title_fullStr Imaging of polysaccharides in the tomato cell wall with Raman microspectroscopy
title_full_unstemmed Imaging of polysaccharides in the tomato cell wall with Raman microspectroscopy
title_short Imaging of polysaccharides in the tomato cell wall with Raman microspectroscopy
title_sort imaging of polysaccharides in the tomato cell wall with raman microspectroscopy
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051370/
https://www.ncbi.nlm.nih.gov/pubmed/24917885
http://dx.doi.org/10.1186/1746-4811-10-14
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