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Genome-Wide Sequencing and an Open Reading Frame Analysis of Dichlorodiphenyltrichloroethane (DDT) Susceptible (91-C) and Resistant (91-R) Drosophila melanogaster Laboratory Populations
The Drosophila melanogaster 91-R and 91-C strains are of common origin, however, 91-R has been intensely selected for dichlorodiphenyltrichloroethane (DDT) resistance over six decades while 91-C has been maintained as the non-selected control strain. These fly strains represent a unique genetic reso...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051598/ https://www.ncbi.nlm.nih.gov/pubmed/24915415 http://dx.doi.org/10.1371/journal.pone.0098584 |
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author | Steele, Laura D. Muir, William M. Seong, Keon Mook Valero, M. Carmen Rangesa, Madhumitha Sun, Weilin Clark, John M. Coates, Brad Pittendrigh, Barry R. |
author_facet | Steele, Laura D. Muir, William M. Seong, Keon Mook Valero, M. Carmen Rangesa, Madhumitha Sun, Weilin Clark, John M. Coates, Brad Pittendrigh, Barry R. |
author_sort | Steele, Laura D. |
collection | PubMed |
description | The Drosophila melanogaster 91-R and 91-C strains are of common origin, however, 91-R has been intensely selected for dichlorodiphenyltrichloroethane (DDT) resistance over six decades while 91-C has been maintained as the non-selected control strain. These fly strains represent a unique genetic resource to understand the accumulation and fixation of mutations under laboratory conditions over decades of pesticide selection. Considerable research has been done to investigate the differential expression of genes associated with the highly DDT resistant strain 91-R, however, with the advent of whole genome sequencing we can now begin to develop an in depth understanding of the genomic changes associated with this intense decades-long xenobiotic selection pressure. Here we present the first whole genome sequencing analysis of the 91-R and 91-C fly strains to identify genome-wide structural changes within the open reading frames. Between-strain changes in allele frequencies revealed a higher percent of new alleles going to fixation for the 91-R strain, as compared to 91-C (P<0.0001). These results suggest that resistance to DDT in the 91-R laboratory strain could potentially be due primarily to new mutations, as well as being polygenic rather than the result of a few major mutations, two hypotheses that remain to be tested. |
format | Online Article Text |
id | pubmed-4051598 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40515982014-06-18 Genome-Wide Sequencing and an Open Reading Frame Analysis of Dichlorodiphenyltrichloroethane (DDT) Susceptible (91-C) and Resistant (91-R) Drosophila melanogaster Laboratory Populations Steele, Laura D. Muir, William M. Seong, Keon Mook Valero, M. Carmen Rangesa, Madhumitha Sun, Weilin Clark, John M. Coates, Brad Pittendrigh, Barry R. PLoS One Research Article The Drosophila melanogaster 91-R and 91-C strains are of common origin, however, 91-R has been intensely selected for dichlorodiphenyltrichloroethane (DDT) resistance over six decades while 91-C has been maintained as the non-selected control strain. These fly strains represent a unique genetic resource to understand the accumulation and fixation of mutations under laboratory conditions over decades of pesticide selection. Considerable research has been done to investigate the differential expression of genes associated with the highly DDT resistant strain 91-R, however, with the advent of whole genome sequencing we can now begin to develop an in depth understanding of the genomic changes associated with this intense decades-long xenobiotic selection pressure. Here we present the first whole genome sequencing analysis of the 91-R and 91-C fly strains to identify genome-wide structural changes within the open reading frames. Between-strain changes in allele frequencies revealed a higher percent of new alleles going to fixation for the 91-R strain, as compared to 91-C (P<0.0001). These results suggest that resistance to DDT in the 91-R laboratory strain could potentially be due primarily to new mutations, as well as being polygenic rather than the result of a few major mutations, two hypotheses that remain to be tested. Public Library of Science 2014-06-10 /pmc/articles/PMC4051598/ /pubmed/24915415 http://dx.doi.org/10.1371/journal.pone.0098584 Text en © 2014 Steele et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Steele, Laura D. Muir, William M. Seong, Keon Mook Valero, M. Carmen Rangesa, Madhumitha Sun, Weilin Clark, John M. Coates, Brad Pittendrigh, Barry R. Genome-Wide Sequencing and an Open Reading Frame Analysis of Dichlorodiphenyltrichloroethane (DDT) Susceptible (91-C) and Resistant (91-R) Drosophila melanogaster Laboratory Populations |
title | Genome-Wide Sequencing and an Open Reading Frame Analysis of Dichlorodiphenyltrichloroethane (DDT) Susceptible (91-C) and Resistant (91-R) Drosophila melanogaster Laboratory Populations |
title_full | Genome-Wide Sequencing and an Open Reading Frame Analysis of Dichlorodiphenyltrichloroethane (DDT) Susceptible (91-C) and Resistant (91-R) Drosophila melanogaster Laboratory Populations |
title_fullStr | Genome-Wide Sequencing and an Open Reading Frame Analysis of Dichlorodiphenyltrichloroethane (DDT) Susceptible (91-C) and Resistant (91-R) Drosophila melanogaster Laboratory Populations |
title_full_unstemmed | Genome-Wide Sequencing and an Open Reading Frame Analysis of Dichlorodiphenyltrichloroethane (DDT) Susceptible (91-C) and Resistant (91-R) Drosophila melanogaster Laboratory Populations |
title_short | Genome-Wide Sequencing and an Open Reading Frame Analysis of Dichlorodiphenyltrichloroethane (DDT) Susceptible (91-C) and Resistant (91-R) Drosophila melanogaster Laboratory Populations |
title_sort | genome-wide sequencing and an open reading frame analysis of dichlorodiphenyltrichloroethane (ddt) susceptible (91-c) and resistant (91-r) drosophila melanogaster laboratory populations |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051598/ https://www.ncbi.nlm.nih.gov/pubmed/24915415 http://dx.doi.org/10.1371/journal.pone.0098584 |
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