Cargando…
Artemisia scoparia Enhances Adipocyte Development and Endocrine Function In Vitro and Enhances Insulin Action In Vivo
BACKGROUND: Failure of adipocytes to expand during periods of energy excess can result in undesirable metabolic consequences such as ectopic fat accumulation and insulin resistance. Blinded screening studies have indicated that Artemisia scoparia (SCO) extracts can enhance adipocyte differentiation...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051605/ https://www.ncbi.nlm.nih.gov/pubmed/24915004 http://dx.doi.org/10.1371/journal.pone.0098897 |
_version_ | 1782320111190802432 |
---|---|
author | Richard, Allison J. Fuller, Scott Fedorcenco, Veaceslav Beyl, Robbie Burris, Thomas P. Mynatt, Randall Ribnicky, David M. Stephens, Jacqueline M. |
author_facet | Richard, Allison J. Fuller, Scott Fedorcenco, Veaceslav Beyl, Robbie Burris, Thomas P. Mynatt, Randall Ribnicky, David M. Stephens, Jacqueline M. |
author_sort | Richard, Allison J. |
collection | PubMed |
description | BACKGROUND: Failure of adipocytes to expand during periods of energy excess can result in undesirable metabolic consequences such as ectopic fat accumulation and insulin resistance. Blinded screening studies have indicated that Artemisia scoparia (SCO) extracts can enhance adipocyte differentiation and lipid accumulation in cultured adipocytes. The present study tested the hypothesis that SCO treatment modulates fat cell development and function in vitro and insulin sensitivity in adipose tissue in vivo. METHODS: In vitro experiments utilized a Gal4-PPARγ ligand binding domain (LBD) fusion protein-luciferase reporter assay to examine PPARγ activation. To investigate the ability of SCO to modulate adipogenesis and mature fat cell function in 3T3-L1 cells, neutral lipid accumulation, gene expression, and protein secretion were measured by Oil Red O staining, qRT-PCR, and immunoblotting, respectively. For the in vivo experiments, diet-induced obese (DIO) C57BL/6J mice were fed a high-fat diet (HFD) or HFD containing 1% w/w SCO for four weeks. Body weight and composition, food intake, and fasting glucose and insulin levels were measured. Phospho-activation and expression of insulin-sensitizing proteins in epididymal adipose tissue (eWAT) were measured by immunoblotting. RESULTS: Ethanolic extracts of A. scoparia significantly activated the PPARγ LBD and enhanced lipid accumulation in differentiating 3T3-L1 cells. SCO increased the transcription of several PPARγ target genes in differentiating 3T3-L1 cells and rescued the negative effects of tumor necrosis factor α on production and secretion of adiponectin and monocyte chemoattractant protein-1 in fully differentiated fat cells. DIO mice treated with SCO had elevated adiponectin levels and increased phosphorylation of AMPKα in eWAT when compared to control mice. In SCO-treated mice, these changes were also associated with decreased fasting insulin and glucose levels. CONCLUSION: SCO has metabolically beneficial effects on adipocytes in vitro and adipose tissue in vivo, highlighting its potential as a metabolically favorable botanical supplement. |
format | Online Article Text |
id | pubmed-4051605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40516052014-06-18 Artemisia scoparia Enhances Adipocyte Development and Endocrine Function In Vitro and Enhances Insulin Action In Vivo Richard, Allison J. Fuller, Scott Fedorcenco, Veaceslav Beyl, Robbie Burris, Thomas P. Mynatt, Randall Ribnicky, David M. Stephens, Jacqueline M. PLoS One Research Article BACKGROUND: Failure of adipocytes to expand during periods of energy excess can result in undesirable metabolic consequences such as ectopic fat accumulation and insulin resistance. Blinded screening studies have indicated that Artemisia scoparia (SCO) extracts can enhance adipocyte differentiation and lipid accumulation in cultured adipocytes. The present study tested the hypothesis that SCO treatment modulates fat cell development and function in vitro and insulin sensitivity in adipose tissue in vivo. METHODS: In vitro experiments utilized a Gal4-PPARγ ligand binding domain (LBD) fusion protein-luciferase reporter assay to examine PPARγ activation. To investigate the ability of SCO to modulate adipogenesis and mature fat cell function in 3T3-L1 cells, neutral lipid accumulation, gene expression, and protein secretion were measured by Oil Red O staining, qRT-PCR, and immunoblotting, respectively. For the in vivo experiments, diet-induced obese (DIO) C57BL/6J mice were fed a high-fat diet (HFD) or HFD containing 1% w/w SCO for four weeks. Body weight and composition, food intake, and fasting glucose and insulin levels were measured. Phospho-activation and expression of insulin-sensitizing proteins in epididymal adipose tissue (eWAT) were measured by immunoblotting. RESULTS: Ethanolic extracts of A. scoparia significantly activated the PPARγ LBD and enhanced lipid accumulation in differentiating 3T3-L1 cells. SCO increased the transcription of several PPARγ target genes in differentiating 3T3-L1 cells and rescued the negative effects of tumor necrosis factor α on production and secretion of adiponectin and monocyte chemoattractant protein-1 in fully differentiated fat cells. DIO mice treated with SCO had elevated adiponectin levels and increased phosphorylation of AMPKα in eWAT when compared to control mice. In SCO-treated mice, these changes were also associated with decreased fasting insulin and glucose levels. CONCLUSION: SCO has metabolically beneficial effects on adipocytes in vitro and adipose tissue in vivo, highlighting its potential as a metabolically favorable botanical supplement. Public Library of Science 2014-06-10 /pmc/articles/PMC4051605/ /pubmed/24915004 http://dx.doi.org/10.1371/journal.pone.0098897 Text en © 2014 Richard et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Richard, Allison J. Fuller, Scott Fedorcenco, Veaceslav Beyl, Robbie Burris, Thomas P. Mynatt, Randall Ribnicky, David M. Stephens, Jacqueline M. Artemisia scoparia Enhances Adipocyte Development and Endocrine Function In Vitro and Enhances Insulin Action In Vivo |
title |
Artemisia scoparia Enhances Adipocyte Development and Endocrine Function In Vitro and Enhances Insulin Action In Vivo
|
title_full |
Artemisia scoparia Enhances Adipocyte Development and Endocrine Function In Vitro and Enhances Insulin Action In Vivo
|
title_fullStr |
Artemisia scoparia Enhances Adipocyte Development and Endocrine Function In Vitro and Enhances Insulin Action In Vivo
|
title_full_unstemmed |
Artemisia scoparia Enhances Adipocyte Development and Endocrine Function In Vitro and Enhances Insulin Action In Vivo
|
title_short |
Artemisia scoparia Enhances Adipocyte Development and Endocrine Function In Vitro and Enhances Insulin Action In Vivo
|
title_sort | artemisia scoparia enhances adipocyte development and endocrine function in vitro and enhances insulin action in vivo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051605/ https://www.ncbi.nlm.nih.gov/pubmed/24915004 http://dx.doi.org/10.1371/journal.pone.0098897 |
work_keys_str_mv | AT richardallisonj artemisiascopariaenhancesadipocytedevelopmentandendocrinefunctioninvitroandenhancesinsulinactioninvivo AT fullerscott artemisiascopariaenhancesadipocytedevelopmentandendocrinefunctioninvitroandenhancesinsulinactioninvivo AT fedorcencoveaceslav artemisiascopariaenhancesadipocytedevelopmentandendocrinefunctioninvitroandenhancesinsulinactioninvivo AT beylrobbie artemisiascopariaenhancesadipocytedevelopmentandendocrinefunctioninvitroandenhancesinsulinactioninvivo AT burristhomasp artemisiascopariaenhancesadipocytedevelopmentandendocrinefunctioninvitroandenhancesinsulinactioninvivo AT mynattrandall artemisiascopariaenhancesadipocytedevelopmentandendocrinefunctioninvitroandenhancesinsulinactioninvivo AT ribnickydavidm artemisiascopariaenhancesadipocytedevelopmentandendocrinefunctioninvitroandenhancesinsulinactioninvivo AT stephensjacquelinem artemisiascopariaenhancesadipocytedevelopmentandendocrinefunctioninvitroandenhancesinsulinactioninvivo |