Cargando…
Source-Identifying Biomarker Ions between Environmental and Clinical Burkholderia pseudomallei Using Whole-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
Burkholderia pseudomallei is the causative agent of melioidosis, which is an endemic disease in Northeast Thailand and Northern Australia. Environmental reservoirs, including wet soils and muddy water, serve as the major sources for contributing bacterial infection to both humans and animals. The wh...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051666/ https://www.ncbi.nlm.nih.gov/pubmed/24914956 http://dx.doi.org/10.1371/journal.pone.0099160 |
_version_ | 1782320123528347648 |
---|---|
author | Niyompanich, Suthamat Jaresitthikunchai, Janthima Srisanga, Kitima Roytrakul, Sittiruk Tungpradabkul, Sumalee |
author_facet | Niyompanich, Suthamat Jaresitthikunchai, Janthima Srisanga, Kitima Roytrakul, Sittiruk Tungpradabkul, Sumalee |
author_sort | Niyompanich, Suthamat |
collection | PubMed |
description | Burkholderia pseudomallei is the causative agent of melioidosis, which is an endemic disease in Northeast Thailand and Northern Australia. Environmental reservoirs, including wet soils and muddy water, serve as the major sources for contributing bacterial infection to both humans and animals. The whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (whole-cell MALDI-TOF MS) has recently been applied as a rapid, accurate, and high-throughput tool for clinical diagnosis and microbiological research. In this present study, we employed a whole-cell MALDI-TOF MS approach for assessing its potency in clustering a total of 11 different B. pseudomallei isolates (consisting of 5 environmental and 6 clinical isolates) with respect to their origins and to further investigate the source-identifying biomarker ions belonging to each bacterial group. The cluster analysis demonstrated that six out of eleven isolates were grouped correctly to their sources. Our results revealed a total of ten source-identifying biomarker ions, which exhibited statistically significant differences in peak intensity between average environmental and clinical mass spectra using ClinProTools software. Six out of ten mass ions were assigned as environmental-identifying biomarker ions (EIBIs), including, m/z 4,056, 4,214, 5,814, 7,545, 7,895, and 8,112, whereas the remaining four mass ions were defined as clinical-identifying biomarker ions (CIBIs) consisting of m/z 3,658, 6,322, 7,035, and 7,984. Hence, our findings represented, for the first time, the source-specific biomarkers of environmental and clinical B. pseudomallei. |
format | Online Article Text |
id | pubmed-4051666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40516662014-06-18 Source-Identifying Biomarker Ions between Environmental and Clinical Burkholderia pseudomallei Using Whole-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Niyompanich, Suthamat Jaresitthikunchai, Janthima Srisanga, Kitima Roytrakul, Sittiruk Tungpradabkul, Sumalee PLoS One Research Article Burkholderia pseudomallei is the causative agent of melioidosis, which is an endemic disease in Northeast Thailand and Northern Australia. Environmental reservoirs, including wet soils and muddy water, serve as the major sources for contributing bacterial infection to both humans and animals. The whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (whole-cell MALDI-TOF MS) has recently been applied as a rapid, accurate, and high-throughput tool for clinical diagnosis and microbiological research. In this present study, we employed a whole-cell MALDI-TOF MS approach for assessing its potency in clustering a total of 11 different B. pseudomallei isolates (consisting of 5 environmental and 6 clinical isolates) with respect to their origins and to further investigate the source-identifying biomarker ions belonging to each bacterial group. The cluster analysis demonstrated that six out of eleven isolates were grouped correctly to their sources. Our results revealed a total of ten source-identifying biomarker ions, which exhibited statistically significant differences in peak intensity between average environmental and clinical mass spectra using ClinProTools software. Six out of ten mass ions were assigned as environmental-identifying biomarker ions (EIBIs), including, m/z 4,056, 4,214, 5,814, 7,545, 7,895, and 8,112, whereas the remaining four mass ions were defined as clinical-identifying biomarker ions (CIBIs) consisting of m/z 3,658, 6,322, 7,035, and 7,984. Hence, our findings represented, for the first time, the source-specific biomarkers of environmental and clinical B. pseudomallei. Public Library of Science 2014-06-10 /pmc/articles/PMC4051666/ /pubmed/24914956 http://dx.doi.org/10.1371/journal.pone.0099160 Text en © 2014 Niyompanich et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Niyompanich, Suthamat Jaresitthikunchai, Janthima Srisanga, Kitima Roytrakul, Sittiruk Tungpradabkul, Sumalee Source-Identifying Biomarker Ions between Environmental and Clinical Burkholderia pseudomallei Using Whole-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) |
title | Source-Identifying Biomarker Ions between Environmental and Clinical Burkholderia pseudomallei Using Whole-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) |
title_full | Source-Identifying Biomarker Ions between Environmental and Clinical Burkholderia pseudomallei Using Whole-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) |
title_fullStr | Source-Identifying Biomarker Ions between Environmental and Clinical Burkholderia pseudomallei Using Whole-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) |
title_full_unstemmed | Source-Identifying Biomarker Ions between Environmental and Clinical Burkholderia pseudomallei Using Whole-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) |
title_short | Source-Identifying Biomarker Ions between Environmental and Clinical Burkholderia pseudomallei Using Whole-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) |
title_sort | source-identifying biomarker ions between environmental and clinical burkholderia pseudomallei using whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051666/ https://www.ncbi.nlm.nih.gov/pubmed/24914956 http://dx.doi.org/10.1371/journal.pone.0099160 |
work_keys_str_mv | AT niyompanichsuthamat sourceidentifyingbiomarkerionsbetweenenvironmentalandclinicalburkholderiapseudomalleiusingwholecellmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometrymalditofms AT jaresitthikunchaijanthima sourceidentifyingbiomarkerionsbetweenenvironmentalandclinicalburkholderiapseudomalleiusingwholecellmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometrymalditofms AT srisangakitima sourceidentifyingbiomarkerionsbetweenenvironmentalandclinicalburkholderiapseudomalleiusingwholecellmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometrymalditofms AT roytrakulsittiruk sourceidentifyingbiomarkerionsbetweenenvironmentalandclinicalburkholderiapseudomalleiusingwholecellmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometrymalditofms AT tungpradabkulsumalee sourceidentifyingbiomarkerionsbetweenenvironmentalandclinicalburkholderiapseudomalleiusingwholecellmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometrymalditofms |