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Hydrogen-Rich Saline Promotes Survival of Retinal Ganglion Cells in a Rat Model of Optic Nerve Crush
OBJECTIVE: To investigate the effect of molecular hydrogen (H(2)) in a rat model subjected to optic nerve crush (ONC). METHODS: We tested the hypothesis that after optic nerve crush (ONC), retinal ganglion cell (RGC) could be protected by H(2). Rats in different groups received saline or hydrogen-ri...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051757/ https://www.ncbi.nlm.nih.gov/pubmed/24915536 http://dx.doi.org/10.1371/journal.pone.0099299 |
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author | Sun, Jing-chuan Xu, Tao Zuo, Qiao Wang, Ruo-bing Qi, Ai-qing Cao, Wen-luo Sun, Ai-jun Sun, Xue-jun Xu, Jiajun |
author_facet | Sun, Jing-chuan Xu, Tao Zuo, Qiao Wang, Ruo-bing Qi, Ai-qing Cao, Wen-luo Sun, Ai-jun Sun, Xue-jun Xu, Jiajun |
author_sort | Sun, Jing-chuan |
collection | PubMed |
description | OBJECTIVE: To investigate the effect of molecular hydrogen (H(2)) in a rat model subjected to optic nerve crush (ONC). METHODS: We tested the hypothesis that after optic nerve crush (ONC), retinal ganglion cell (RGC) could be protected by H(2). Rats in different groups received saline or hydrogen-rich saline every day for 14 days after ONC. Retinas from animals in each group underwent measurements of hematoxylin and eosin (H&E) staining, cholera toxin beta (CTB) tracing, gamma synuclein staining, and terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) staining 2 weeks post operation. Flash visual evoked potentials (FVEP) and pupillary light reflex (PLR) were then tested to evaluate the function of optic nerve. The malondialdehyde (MDA) level in retina was evaluated. RESULTS: H&E, gamma synuclein staining and CTB tracing showed that the survival rate of RGCs in hydrogen saline-treated group was significantly higher than that in saline-treated group. Apoptosis of RGCs assessed by TUNEL staining were less observed in hydrogen saline-treated group. The MDA level in retina of H(2) group was much lower than that in placebo group. Furthermore, animals treated with hydrogen saline showed better function of optic nerve in assessments of FVEP and PLR. CONCLUSION: These results demonstrated that H(2) protects RGCs and helps preserve the visual function after ONC and had a neuroprotective effect in a rat model subjected to ONC. |
format | Online Article Text |
id | pubmed-4051757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40517572014-06-18 Hydrogen-Rich Saline Promotes Survival of Retinal Ganglion Cells in a Rat Model of Optic Nerve Crush Sun, Jing-chuan Xu, Tao Zuo, Qiao Wang, Ruo-bing Qi, Ai-qing Cao, Wen-luo Sun, Ai-jun Sun, Xue-jun Xu, Jiajun PLoS One Research Article OBJECTIVE: To investigate the effect of molecular hydrogen (H(2)) in a rat model subjected to optic nerve crush (ONC). METHODS: We tested the hypothesis that after optic nerve crush (ONC), retinal ganglion cell (RGC) could be protected by H(2). Rats in different groups received saline or hydrogen-rich saline every day for 14 days after ONC. Retinas from animals in each group underwent measurements of hematoxylin and eosin (H&E) staining, cholera toxin beta (CTB) tracing, gamma synuclein staining, and terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) staining 2 weeks post operation. Flash visual evoked potentials (FVEP) and pupillary light reflex (PLR) were then tested to evaluate the function of optic nerve. The malondialdehyde (MDA) level in retina was evaluated. RESULTS: H&E, gamma synuclein staining and CTB tracing showed that the survival rate of RGCs in hydrogen saline-treated group was significantly higher than that in saline-treated group. Apoptosis of RGCs assessed by TUNEL staining were less observed in hydrogen saline-treated group. The MDA level in retina of H(2) group was much lower than that in placebo group. Furthermore, animals treated with hydrogen saline showed better function of optic nerve in assessments of FVEP and PLR. CONCLUSION: These results demonstrated that H(2) protects RGCs and helps preserve the visual function after ONC and had a neuroprotective effect in a rat model subjected to ONC. Public Library of Science 2014-06-10 /pmc/articles/PMC4051757/ /pubmed/24915536 http://dx.doi.org/10.1371/journal.pone.0099299 Text en © 2014 Sun et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Sun, Jing-chuan Xu, Tao Zuo, Qiao Wang, Ruo-bing Qi, Ai-qing Cao, Wen-luo Sun, Ai-jun Sun, Xue-jun Xu, Jiajun Hydrogen-Rich Saline Promotes Survival of Retinal Ganglion Cells in a Rat Model of Optic Nerve Crush |
title | Hydrogen-Rich Saline Promotes Survival of Retinal Ganglion Cells in a Rat Model of Optic Nerve Crush |
title_full | Hydrogen-Rich Saline Promotes Survival of Retinal Ganglion Cells in a Rat Model of Optic Nerve Crush |
title_fullStr | Hydrogen-Rich Saline Promotes Survival of Retinal Ganglion Cells in a Rat Model of Optic Nerve Crush |
title_full_unstemmed | Hydrogen-Rich Saline Promotes Survival of Retinal Ganglion Cells in a Rat Model of Optic Nerve Crush |
title_short | Hydrogen-Rich Saline Promotes Survival of Retinal Ganglion Cells in a Rat Model of Optic Nerve Crush |
title_sort | hydrogen-rich saline promotes survival of retinal ganglion cells in a rat model of optic nerve crush |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051757/ https://www.ncbi.nlm.nih.gov/pubmed/24915536 http://dx.doi.org/10.1371/journal.pone.0099299 |
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