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Monitoring Protein Misfolding by Site-Specific Labeling of Proteins In Vivo
Incorporating fluorescent amino acids by suppression of the TAG amber codon is a useful tool for site-specific labeling of proteins and visualizing their localization in living cells. Here we use a plasmid encoded orthogonal tRNA/aminoacyl-tRNA synthetase pair to site-specifically label firefly luci...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051779/ https://www.ncbi.nlm.nih.gov/pubmed/24915041 http://dx.doi.org/10.1371/journal.pone.0099395 |