Two missense mutations, E123Q and K151E, identified in the ERG11 allele of an azole-resistant isolate of Candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia

We report on the first cloning and nucleotide sequencing of an ERG11 allele from a clinical isolate of Candida kefyr cross-resistant to azole antifungals. It was recovered from a stem cell transplant patient, in an oncohematology unit exhibiting unexpected high prevalence of C. kefyr. Two amino acid...

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Autores principales: Couzigou, Célia, Gabriel, Frédéric, Biteau, Nicolas, Fitton-Ouhabi, Valérie, Noël, Thierry, Accoceberry, Isabelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052357/
https://www.ncbi.nlm.nih.gov/pubmed/24936404
http://dx.doi.org/10.1016/j.mmcr.2014.04.002
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author Couzigou, Célia
Gabriel, Frédéric
Biteau, Nicolas
Fitton-Ouhabi, Valérie
Noël, Thierry
Accoceberry, Isabelle
author_facet Couzigou, Célia
Gabriel, Frédéric
Biteau, Nicolas
Fitton-Ouhabi, Valérie
Noël, Thierry
Accoceberry, Isabelle
author_sort Couzigou, Célia
collection PubMed
description We report on the first cloning and nucleotide sequencing of an ERG11 allele from a clinical isolate of Candida kefyr cross-resistant to azole antifungals. It was recovered from a stem cell transplant patient, in an oncohematology unit exhibiting unexpected high prevalence of C. kefyr. Two amino acid substitutions were identified: K151E, whose role in fluconazole resistance was already demonstrated in Candida albicans, and E123Q, a new substitution never described so far in azole-resistant Candida yeast.
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spelling pubmed-40523572014-06-16 Two missense mutations, E123Q and K151E, identified in the ERG11 allele of an azole-resistant isolate of Candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia Couzigou, Célia Gabriel, Frédéric Biteau, Nicolas Fitton-Ouhabi, Valérie Noël, Thierry Accoceberry, Isabelle Med Mycol Case Rep Article We report on the first cloning and nucleotide sequencing of an ERG11 allele from a clinical isolate of Candida kefyr cross-resistant to azole antifungals. It was recovered from a stem cell transplant patient, in an oncohematology unit exhibiting unexpected high prevalence of C. kefyr. Two amino acid substitutions were identified: K151E, whose role in fluconazole resistance was already demonstrated in Candida albicans, and E123Q, a new substitution never described so far in azole-resistant Candida yeast. Elsevier 2014-05-09 /pmc/articles/PMC4052357/ /pubmed/24936404 http://dx.doi.org/10.1016/j.mmcr.2014.04.002 Text en © 2014 International Society for Human and Animal Mycology http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Couzigou, Célia
Gabriel, Frédéric
Biteau, Nicolas
Fitton-Ouhabi, Valérie
Noël, Thierry
Accoceberry, Isabelle
Two missense mutations, E123Q and K151E, identified in the ERG11 allele of an azole-resistant isolate of Candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia
title Two missense mutations, E123Q and K151E, identified in the ERG11 allele of an azole-resistant isolate of Candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia
title_full Two missense mutations, E123Q and K151E, identified in the ERG11 allele of an azole-resistant isolate of Candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia
title_fullStr Two missense mutations, E123Q and K151E, identified in the ERG11 allele of an azole-resistant isolate of Candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia
title_full_unstemmed Two missense mutations, E123Q and K151E, identified in the ERG11 allele of an azole-resistant isolate of Candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia
title_short Two missense mutations, E123Q and K151E, identified in the ERG11 allele of an azole-resistant isolate of Candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia
title_sort two missense mutations, e123q and k151e, identified in the erg11 allele of an azole-resistant isolate of candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052357/
https://www.ncbi.nlm.nih.gov/pubmed/24936404
http://dx.doi.org/10.1016/j.mmcr.2014.04.002
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