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Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2
Cell migration is an integral part of re-epithelialization during skin wound healing, a complex process involving molecular controls that are still largely unknown. Here we identify a novel role for Tcf3, an essential transcription factor regulating embryonic and adult skin stem cell functions, as a...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052366/ https://www.ncbi.nlm.nih.gov/pubmed/24909826 http://dx.doi.org/10.1038/ncomms5088 |
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author | Miao, Qi Ku, Amy T. Nishino, Yudai Howard, Jeffrey M. Rao, Ajay S. Shaver, Timothy M. Garcia, Gloria E. Le, Diep N. Karlin, Kristen L. Westbrook, Thomas F. Poli, Valeria Nguyen, Hoang |
author_facet | Miao, Qi Ku, Amy T. Nishino, Yudai Howard, Jeffrey M. Rao, Ajay S. Shaver, Timothy M. Garcia, Gloria E. Le, Diep N. Karlin, Kristen L. Westbrook, Thomas F. Poli, Valeria Nguyen, Hoang |
author_sort | Miao, Qi |
collection | PubMed |
description | Cell migration is an integral part of re-epithelialization during skin wound healing, a complex process involving molecular controls that are still largely unknown. Here we identify a novel role for Tcf3, an essential transcription factor regulating embryonic and adult skin stem cell functions, as a key effector of epidermal wound repair. We show that Tcf3 is upregulated in skin wounds and that Tcf3 overexpression accelerates keratinocyte migration and skin wound healing. We also identify Stat3 as an upstream regulator of Tcf3. We show that the pro-migration effects of Tcf3 are non-cell autonomous and occur independently of its ability to interact with β-catenin. Finally, we identify lipocalin-2 as the key secreted factor downstream of Tcf3 that promotes cell migration in vitro and wound healing in vivo. Our findings provide new insights into the molecular controls of wound-associated cell migration and identify potential therapeutic targets for the treatment of defective wound repair. |
format | Online Article Text |
id | pubmed-4052366 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-40523662014-12-09 Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2 Miao, Qi Ku, Amy T. Nishino, Yudai Howard, Jeffrey M. Rao, Ajay S. Shaver, Timothy M. Garcia, Gloria E. Le, Diep N. Karlin, Kristen L. Westbrook, Thomas F. Poli, Valeria Nguyen, Hoang Nat Commun Article Cell migration is an integral part of re-epithelialization during skin wound healing, a complex process involving molecular controls that are still largely unknown. Here we identify a novel role for Tcf3, an essential transcription factor regulating embryonic and adult skin stem cell functions, as a key effector of epidermal wound repair. We show that Tcf3 is upregulated in skin wounds and that Tcf3 overexpression accelerates keratinocyte migration and skin wound healing. We also identify Stat3 as an upstream regulator of Tcf3. We show that the pro-migration effects of Tcf3 are non-cell autonomous and occur independently of its ability to interact with β-catenin. Finally, we identify lipocalin-2 as the key secreted factor downstream of Tcf3 that promotes cell migration in vitro and wound healing in vivo. Our findings provide new insights into the molecular controls of wound-associated cell migration and identify potential therapeutic targets for the treatment of defective wound repair. 2014-06-09 /pmc/articles/PMC4052366/ /pubmed/24909826 http://dx.doi.org/10.1038/ncomms5088 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Miao, Qi Ku, Amy T. Nishino, Yudai Howard, Jeffrey M. Rao, Ajay S. Shaver, Timothy M. Garcia, Gloria E. Le, Diep N. Karlin, Kristen L. Westbrook, Thomas F. Poli, Valeria Nguyen, Hoang Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2 |
title | Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2 |
title_full | Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2 |
title_fullStr | Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2 |
title_full_unstemmed | Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2 |
title_short | Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2 |
title_sort | tcf3 promotes cell migration and wound repair through regulation of lipocalin 2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052366/ https://www.ncbi.nlm.nih.gov/pubmed/24909826 http://dx.doi.org/10.1038/ncomms5088 |
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