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Genetic and physiological analysis of tolerance to acute iron toxicity in rice

BACKGROUND: Fe toxicity occurs in lowland rice production due to excess ferrous iron (Fe(2+)) formation in reduced soils. To contribute to the breeding for tolerance to Fe toxicity in rice, we determined quantitative trait loci (QTL) by screening two different bi-parental mapping populations under i...

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Autores principales: Wu, Lin-Bo, Shhadi, Mohamad Yusser, Gregorio, Glenn, Matthus, Elsa, Becker, Mathias, Frei, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052628/
https://www.ncbi.nlm.nih.gov/pubmed/24920973
http://dx.doi.org/10.1186/s12284-014-0008-3
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author Wu, Lin-Bo
Shhadi, Mohamad Yusser
Gregorio, Glenn
Matthus, Elsa
Becker, Mathias
Frei, Michael
author_facet Wu, Lin-Bo
Shhadi, Mohamad Yusser
Gregorio, Glenn
Matthus, Elsa
Becker, Mathias
Frei, Michael
author_sort Wu, Lin-Bo
collection PubMed
description BACKGROUND: Fe toxicity occurs in lowland rice production due to excess ferrous iron (Fe(2+)) formation in reduced soils. To contribute to the breeding for tolerance to Fe toxicity in rice, we determined quantitative trait loci (QTL) by screening two different bi-parental mapping populations under iron pulse stresses (1,000 mg L(−1) = 17.9 mM Fe(2+) for 5 days) in hydroponic solution, followed by experiments with selected lines to determine whether QTLs were associated with iron exclusion (i.e. root based mechanisms), or iron inclusion (i.e. shoot-based mechanisms). RESULTS: In an IR29/Pokkali F(8) recombinant inbred population, 7 QTLs were detected for leaf bronzing score on chromosome 1, 2, 4, 7 and 12, respectively, individually explaining 9.2-18.7% of the phenotypic variation. Two tolerant recombinant inbred lines carrying putative QTLs were selected for further experiments. Based on Fe uptake into the shoot, the dominant tolerance mechanism of the tolerant line FL510 was determined to be exclusion with its root architecture being conducive to air transport and thus the ability to oxidize Fe(2+) in rhizosphere. In line FL483, the iron tolerance was related mainly to shoot-based mechanisms (tolerant inclusion mechanism). In a Nipponbare/Kasalath/Nipponbare backcross inbred population, 3 QTLs were mapped on chromosomes 1, 3 and 8, respectively. These QTLs explained 11.6-18.6% of the total phenotypic variation. The effect of QTLs on chromosome 1 and 3 were confirmed by using chromosome segment substitution lines (SL), carrying Kasalath introgressions in the genetic background on Nipponbare. The Fe uptake in shoots of substitution lines suggests that the effect of the QTL on chromosome 1 was associated with shoot tolerance while the QTL on chromosome 3 was associated with iron exclusion. CONCLUSION: Tolerance of certain genotypes were classified into shoot- and root- based mechanisms. Comparing our findings with previously reported QTLs for iron toxicity tolerance, we identified co-localization for some QTLs in both pluse and chronic stresses, especially on chromosome 1.
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spelling pubmed-40526282014-06-12 Genetic and physiological analysis of tolerance to acute iron toxicity in rice Wu, Lin-Bo Shhadi, Mohamad Yusser Gregorio, Glenn Matthus, Elsa Becker, Mathias Frei, Michael Rice (N Y) Research BACKGROUND: Fe toxicity occurs in lowland rice production due to excess ferrous iron (Fe(2+)) formation in reduced soils. To contribute to the breeding for tolerance to Fe toxicity in rice, we determined quantitative trait loci (QTL) by screening two different bi-parental mapping populations under iron pulse stresses (1,000 mg L(−1) = 17.9 mM Fe(2+) for 5 days) in hydroponic solution, followed by experiments with selected lines to determine whether QTLs were associated with iron exclusion (i.e. root based mechanisms), or iron inclusion (i.e. shoot-based mechanisms). RESULTS: In an IR29/Pokkali F(8) recombinant inbred population, 7 QTLs were detected for leaf bronzing score on chromosome 1, 2, 4, 7 and 12, respectively, individually explaining 9.2-18.7% of the phenotypic variation. Two tolerant recombinant inbred lines carrying putative QTLs were selected for further experiments. Based on Fe uptake into the shoot, the dominant tolerance mechanism of the tolerant line FL510 was determined to be exclusion with its root architecture being conducive to air transport and thus the ability to oxidize Fe(2+) in rhizosphere. In line FL483, the iron tolerance was related mainly to shoot-based mechanisms (tolerant inclusion mechanism). In a Nipponbare/Kasalath/Nipponbare backcross inbred population, 3 QTLs were mapped on chromosomes 1, 3 and 8, respectively. These QTLs explained 11.6-18.6% of the total phenotypic variation. The effect of QTLs on chromosome 1 and 3 were confirmed by using chromosome segment substitution lines (SL), carrying Kasalath introgressions in the genetic background on Nipponbare. The Fe uptake in shoots of substitution lines suggests that the effect of the QTL on chromosome 1 was associated with shoot tolerance while the QTL on chromosome 3 was associated with iron exclusion. CONCLUSION: Tolerance of certain genotypes were classified into shoot- and root- based mechanisms. Comparing our findings with previously reported QTLs for iron toxicity tolerance, we identified co-localization for some QTLs in both pluse and chronic stresses, especially on chromosome 1. Springer 2014-05-30 /pmc/articles/PMC4052628/ /pubmed/24920973 http://dx.doi.org/10.1186/s12284-014-0008-3 Text en Copyright © 2014 Wu et al.; licensee Springer. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research
Wu, Lin-Bo
Shhadi, Mohamad Yusser
Gregorio, Glenn
Matthus, Elsa
Becker, Mathias
Frei, Michael
Genetic and physiological analysis of tolerance to acute iron toxicity in rice
title Genetic and physiological analysis of tolerance to acute iron toxicity in rice
title_full Genetic and physiological analysis of tolerance to acute iron toxicity in rice
title_fullStr Genetic and physiological analysis of tolerance to acute iron toxicity in rice
title_full_unstemmed Genetic and physiological analysis of tolerance to acute iron toxicity in rice
title_short Genetic and physiological analysis of tolerance to acute iron toxicity in rice
title_sort genetic and physiological analysis of tolerance to acute iron toxicity in rice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052628/
https://www.ncbi.nlm.nih.gov/pubmed/24920973
http://dx.doi.org/10.1186/s12284-014-0008-3
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