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RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination

RNA-binding protein CsrA is a key regulator of a variety of cellular processes in bacteria, including carbon and stationary phase metabolism, biofilm formation, quorum sensing, and virulence gene expression in pathogens. CsrA binds to bipartite sequence elements at or near the ribosome loading site...

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Autores principales: Figueroa-Bossi, Nara, Schwartz, Annie, Guillemardet, Benoit, D’Heygère, François, Bossi, Lionello, Boudvillain, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052769/
https://www.ncbi.nlm.nih.gov/pubmed/24888591
http://dx.doi.org/10.1101/gad.240192.114
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author Figueroa-Bossi, Nara
Schwartz, Annie
Guillemardet, Benoit
D’Heygère, François
Bossi, Lionello
Boudvillain, Marc
author_facet Figueroa-Bossi, Nara
Schwartz, Annie
Guillemardet, Benoit
D’Heygère, François
Bossi, Lionello
Boudvillain, Marc
author_sort Figueroa-Bossi, Nara
collection PubMed
description RNA-binding protein CsrA is a key regulator of a variety of cellular processes in bacteria, including carbon and stationary phase metabolism, biofilm formation, quorum sensing, and virulence gene expression in pathogens. CsrA binds to bipartite sequence elements at or near the ribosome loading site in messenger RNA (mRNA), most often inhibiting translation initiation. Here we describe an alternative novel mechanism through which CsrA achieves negative regulation. We show that CsrA binding to the upstream portion of the 5′ untranslated region of Escherichia coli pgaA mRNA—encoding a polysaccharide adhesin export protein—unfolds a secondary structure that sequesters an entry site for transcription termination factor Rho, resulting in the premature stop of transcription. These findings establish a new paradigm for bacterial gene regulation in which remodeling of the nascent transcript by a regulatory protein promotes Rho-dependent transcription attenuation.
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spelling pubmed-40527692014-12-01 RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination Figueroa-Bossi, Nara Schwartz, Annie Guillemardet, Benoit D’Heygère, François Bossi, Lionello Boudvillain, Marc Genes Dev Research Paper RNA-binding protein CsrA is a key regulator of a variety of cellular processes in bacteria, including carbon and stationary phase metabolism, biofilm formation, quorum sensing, and virulence gene expression in pathogens. CsrA binds to bipartite sequence elements at or near the ribosome loading site in messenger RNA (mRNA), most often inhibiting translation initiation. Here we describe an alternative novel mechanism through which CsrA achieves negative regulation. We show that CsrA binding to the upstream portion of the 5′ untranslated region of Escherichia coli pgaA mRNA—encoding a polysaccharide adhesin export protein—unfolds a secondary structure that sequesters an entry site for transcription termination factor Rho, resulting in the premature stop of transcription. These findings establish a new paradigm for bacterial gene regulation in which remodeling of the nascent transcript by a regulatory protein promotes Rho-dependent transcription attenuation. Cold Spring Harbor Laboratory Press 2014-06-01 /pmc/articles/PMC4052769/ /pubmed/24888591 http://dx.doi.org/10.1101/gad.240192.114 Text en © 2014 Figueroa-Bossi et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Paper
Figueroa-Bossi, Nara
Schwartz, Annie
Guillemardet, Benoit
D’Heygère, François
Bossi, Lionello
Boudvillain, Marc
RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination
title RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination
title_full RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination
title_fullStr RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination
title_full_unstemmed RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination
title_short RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination
title_sort rna remodeling by bacterial global regulator csra promotes rho-dependent transcription termination
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052769/
https://www.ncbi.nlm.nih.gov/pubmed/24888591
http://dx.doi.org/10.1101/gad.240192.114
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