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Effects of biosurfactants on the viability and proliferation of human breast cancer cells

Biosurfactants are molecules with surface activity produced by microorganisms that can be used in many biomedical applications. The anti-tumour potential of these molecules is being studied, although results are still scarce and few data are available regarding the mechanisms underlying such activit...

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Detalles Bibliográficos
Autores principales: Duarte, Cristina, Gudiña, Eduardo J, Lima, Cristovao F, Rodrigues, Ligia R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052778/
https://www.ncbi.nlm.nih.gov/pubmed/24949273
http://dx.doi.org/10.1186/s13568-014-0040-0
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author Duarte, Cristina
Gudiña, Eduardo J
Lima, Cristovao F
Rodrigues, Ligia R
author_facet Duarte, Cristina
Gudiña, Eduardo J
Lima, Cristovao F
Rodrigues, Ligia R
author_sort Duarte, Cristina
collection PubMed
description Biosurfactants are molecules with surface activity produced by microorganisms that can be used in many biomedical applications. The anti-tumour potential of these molecules is being studied, although results are still scarce and few data are available regarding the mechanisms underlying such activity. In this work, the anti-tumour activity of a surfactin produced by Bacillus subtilis 573 and a glycoprotein (BioEG) produced by Lactobacillus paracasei subsp. paracasei A20 was evaluated. Both biosurfactants were tested against two breast cancer cell lines, T47D and MDA-MB-231, and a non-tumour fibroblast cell line (MC-3 T3-E1), specifically regarding cell viability and proliferation. Surfactin was found to decrease viability of both breast cancer cell lines studied. A 24 h exposure to 0.05 g l(-1) surfactin led to inhibition of cell proliferation as shown by cell cycle arrest at G1 phase. Similarly, exposure of cells to 0.15 g l(-1) BioEG for 48 h decreased cancer cells’ viability, without affecting normal fibroblasts. Moreover, BioEG induced the cell cycle arrest at G1 for both breast cancer cell lines. The biosurfactant BioEG was shown to be more active than surfactin against the studied breast cancer cells. The results gathered in this work are very promising regarding the biosurfactants potential for breast cancer treatment and encourage further work with the BioEG glycoprotein.
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spelling pubmed-40527782014-06-19 Effects of biosurfactants on the viability and proliferation of human breast cancer cells Duarte, Cristina Gudiña, Eduardo J Lima, Cristovao F Rodrigues, Ligia R AMB Express Research Article Biosurfactants are molecules with surface activity produced by microorganisms that can be used in many biomedical applications. The anti-tumour potential of these molecules is being studied, although results are still scarce and few data are available regarding the mechanisms underlying such activity. In this work, the anti-tumour activity of a surfactin produced by Bacillus subtilis 573 and a glycoprotein (BioEG) produced by Lactobacillus paracasei subsp. paracasei A20 was evaluated. Both biosurfactants were tested against two breast cancer cell lines, T47D and MDA-MB-231, and a non-tumour fibroblast cell line (MC-3 T3-E1), specifically regarding cell viability and proliferation. Surfactin was found to decrease viability of both breast cancer cell lines studied. A 24 h exposure to 0.05 g l(-1) surfactin led to inhibition of cell proliferation as shown by cell cycle arrest at G1 phase. Similarly, exposure of cells to 0.15 g l(-1) BioEG for 48 h decreased cancer cells’ viability, without affecting normal fibroblasts. Moreover, BioEG induced the cell cycle arrest at G1 for both breast cancer cell lines. The biosurfactant BioEG was shown to be more active than surfactin against the studied breast cancer cells. The results gathered in this work are very promising regarding the biosurfactants potential for breast cancer treatment and encourage further work with the BioEG glycoprotein. Springer 2014-04-15 /pmc/articles/PMC4052778/ /pubmed/24949273 http://dx.doi.org/10.1186/s13568-014-0040-0 Text en Copyright © 2014 Duarte et al.; licensee Springer http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research Article
Duarte, Cristina
Gudiña, Eduardo J
Lima, Cristovao F
Rodrigues, Ligia R
Effects of biosurfactants on the viability and proliferation of human breast cancer cells
title Effects of biosurfactants on the viability and proliferation of human breast cancer cells
title_full Effects of biosurfactants on the viability and proliferation of human breast cancer cells
title_fullStr Effects of biosurfactants on the viability and proliferation of human breast cancer cells
title_full_unstemmed Effects of biosurfactants on the viability and proliferation of human breast cancer cells
title_short Effects of biosurfactants on the viability and proliferation of human breast cancer cells
title_sort effects of biosurfactants on the viability and proliferation of human breast cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052778/
https://www.ncbi.nlm.nih.gov/pubmed/24949273
http://dx.doi.org/10.1186/s13568-014-0040-0
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