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Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering

We investigated the feasibility of oleuropein as a cross-linking agent for fabricating three-dimensional (3D) porous composite scaffolds for bone tissue engineering. Human-like collagen (HLC) and nanohydroxyapatite (n-HAp) were used to fabricate the composite scaffold by way of cross-linking. The me...

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Detalles Bibliográficos
Autores principales: Fan, Hui, Hui, Junfeng, Duan, Zhiguang, Fan, Daidi, Mi, Yu, Deng, Jianjun, Li, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052823/
https://www.ncbi.nlm.nih.gov/pubmed/24959582
http://dx.doi.org/10.1155/2014/652432
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author Fan, Hui
Hui, Junfeng
Duan, Zhiguang
Fan, Daidi
Mi, Yu
Deng, Jianjun
Li, Hui
author_facet Fan, Hui
Hui, Junfeng
Duan, Zhiguang
Fan, Daidi
Mi, Yu
Deng, Jianjun
Li, Hui
author_sort Fan, Hui
collection PubMed
description We investigated the feasibility of oleuropein as a cross-linking agent for fabricating three-dimensional (3D) porous composite scaffolds for bone tissue engineering. Human-like collagen (HLC) and nanohydroxyapatite (n-HAp) were used to fabricate the composite scaffold by way of cross-linking. The mechanical tests revealed superior properties for the cross-linked scaffolds compared to the uncross-linked scaffolds. The as-obtained composite scaffold had a 3D porous structure with pores ranging from 120 to 300 μm and a porosity of 73.6 ± 2.3%. The cross-linked scaffolds were seeded with MC3T3-E1 Subclone 14 mouse osteoblasts. Fluorescence staining, the Cell Counting Kit-8 (CCK-8) assay, and scanning electron microscopy (SEM) indicated that the scaffolds enhanced cell adhesion and proliferation. Our results indicate the potential of these scaffolds for bone tissue engineering.
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spelling pubmed-40528232014-06-23 Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering Fan, Hui Hui, Junfeng Duan, Zhiguang Fan, Daidi Mi, Yu Deng, Jianjun Li, Hui Biomed Res Int Research Article We investigated the feasibility of oleuropein as a cross-linking agent for fabricating three-dimensional (3D) porous composite scaffolds for bone tissue engineering. Human-like collagen (HLC) and nanohydroxyapatite (n-HAp) were used to fabricate the composite scaffold by way of cross-linking. The mechanical tests revealed superior properties for the cross-linked scaffolds compared to the uncross-linked scaffolds. The as-obtained composite scaffold had a 3D porous structure with pores ranging from 120 to 300 μm and a porosity of 73.6 ± 2.3%. The cross-linked scaffolds were seeded with MC3T3-E1 Subclone 14 mouse osteoblasts. Fluorescence staining, the Cell Counting Kit-8 (CCK-8) assay, and scanning electron microscopy (SEM) indicated that the scaffolds enhanced cell adhesion and proliferation. Our results indicate the potential of these scaffolds for bone tissue engineering. Hindawi Publishing Corporation 2014 2014-05-13 /pmc/articles/PMC4052823/ /pubmed/24959582 http://dx.doi.org/10.1155/2014/652432 Text en Copyright © 2014 Hui Fan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Fan, Hui
Hui, Junfeng
Duan, Zhiguang
Fan, Daidi
Mi, Yu
Deng, Jianjun
Li, Hui
Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering
title Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering
title_full Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering
title_fullStr Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering
title_full_unstemmed Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering
title_short Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering
title_sort novel scaffolds fabricated using oleuropein for bone tissue engineering
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052823/
https://www.ncbi.nlm.nih.gov/pubmed/24959582
http://dx.doi.org/10.1155/2014/652432
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