Cargando…
Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study
Cisplatin (CisPt) is a widely used chemotherapeutic drug whose side effects include muscle weakness and cachexia. Here we analysed CisPt-induced atrophy in C2C12 myotubes by a multidisciplinary morphological approach, focusing on the onset and progression of autophagy, a protective cellular process...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053152/ https://www.ncbi.nlm.nih.gov/pubmed/24955211 http://dx.doi.org/10.1155/2014/840951 |
_version_ | 1782320332187631616 |
---|---|
author | Stacchiotti, Alessandra Rovetta, Francesca Ferroni, Matteo Corsetti, Giovanni Lavazza, Antonio Sberveglieri, Giorgio Aleo, Maria Francesca |
author_facet | Stacchiotti, Alessandra Rovetta, Francesca Ferroni, Matteo Corsetti, Giovanni Lavazza, Antonio Sberveglieri, Giorgio Aleo, Maria Francesca |
author_sort | Stacchiotti, Alessandra |
collection | PubMed |
description | Cisplatin (CisPt) is a widely used chemotherapeutic drug whose side effects include muscle weakness and cachexia. Here we analysed CisPt-induced atrophy in C2C12 myotubes by a multidisciplinary morphological approach, focusing on the onset and progression of autophagy, a protective cellular process that, when excessively activated, may trigger protein hypercatabolism and atrophy in skeletal muscle. To visualize autophagy we used confocal and transmission electron microscopy at different times of treatment and doses of CisPt. Moreover we evaluated the effects of taurine, a cytoprotective beta-amino acid able to counteract oxidative stress, apoptosis, and endoplasmic reticulum stress in different tissues and organs. Our microscopic results indicate that autophagy occurs very early in 50 μM CisPt challenged myotubes (4 h–8 h) before overt atrophy but it persists even at 24 h, when several autophagic vesicles, damaged mitochondria, and sarcoplasmic blebbings engulf the sarcoplasm. Differently, 25 mM taurine pretreatment rescues the majority of myotubes size upon 50 μM CisPt at 24 h. Taurine appears to counteract atrophy by restoring regular microtubular apparatus and mitochondria and reducing the overload and the localization of autophagolysosomes. Such a promising taurine action in preventing atrophy needs further molecular and biochemical studies to best define its impact on muscle homeostasis and the maintenance of an adequate skeletal mass in vivo. |
format | Online Article Text |
id | pubmed-4053152 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-40531522014-06-22 Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study Stacchiotti, Alessandra Rovetta, Francesca Ferroni, Matteo Corsetti, Giovanni Lavazza, Antonio Sberveglieri, Giorgio Aleo, Maria Francesca Oxid Med Cell Longev Research Article Cisplatin (CisPt) is a widely used chemotherapeutic drug whose side effects include muscle weakness and cachexia. Here we analysed CisPt-induced atrophy in C2C12 myotubes by a multidisciplinary morphological approach, focusing on the onset and progression of autophagy, a protective cellular process that, when excessively activated, may trigger protein hypercatabolism and atrophy in skeletal muscle. To visualize autophagy we used confocal and transmission electron microscopy at different times of treatment and doses of CisPt. Moreover we evaluated the effects of taurine, a cytoprotective beta-amino acid able to counteract oxidative stress, apoptosis, and endoplasmic reticulum stress in different tissues and organs. Our microscopic results indicate that autophagy occurs very early in 50 μM CisPt challenged myotubes (4 h–8 h) before overt atrophy but it persists even at 24 h, when several autophagic vesicles, damaged mitochondria, and sarcoplasmic blebbings engulf the sarcoplasm. Differently, 25 mM taurine pretreatment rescues the majority of myotubes size upon 50 μM CisPt at 24 h. Taurine appears to counteract atrophy by restoring regular microtubular apparatus and mitochondria and reducing the overload and the localization of autophagolysosomes. Such a promising taurine action in preventing atrophy needs further molecular and biochemical studies to best define its impact on muscle homeostasis and the maintenance of an adequate skeletal mass in vivo. Hindawi Publishing Corporation 2014 2014-05-13 /pmc/articles/PMC4053152/ /pubmed/24955211 http://dx.doi.org/10.1155/2014/840951 Text en Copyright © 2014 Alessandra Stacchiotti et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Stacchiotti, Alessandra Rovetta, Francesca Ferroni, Matteo Corsetti, Giovanni Lavazza, Antonio Sberveglieri, Giorgio Aleo, Maria Francesca Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study |
title | Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study |
title_full | Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study |
title_fullStr | Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study |
title_full_unstemmed | Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study |
title_short | Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study |
title_sort | taurine rescues cisplatin-induced muscle atrophy in vitro: a morphological study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053152/ https://www.ncbi.nlm.nih.gov/pubmed/24955211 http://dx.doi.org/10.1155/2014/840951 |
work_keys_str_mv | AT stacchiottialessandra taurinerescuescisplatininducedmuscleatrophyinvitroamorphologicalstudy AT rovettafrancesca taurinerescuescisplatininducedmuscleatrophyinvitroamorphologicalstudy AT ferronimatteo taurinerescuescisplatininducedmuscleatrophyinvitroamorphologicalstudy AT corsettigiovanni taurinerescuescisplatininducedmuscleatrophyinvitroamorphologicalstudy AT lavazzaantonio taurinerescuescisplatininducedmuscleatrophyinvitroamorphologicalstudy AT sberveglierigiorgio taurinerescuescisplatininducedmuscleatrophyinvitroamorphologicalstudy AT aleomariafrancesca taurinerescuescisplatininducedmuscleatrophyinvitroamorphologicalstudy |