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Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study

Cisplatin (CisPt) is a widely used chemotherapeutic drug whose side effects include muscle weakness and cachexia. Here we analysed CisPt-induced atrophy in C2C12 myotubes by a multidisciplinary morphological approach, focusing on the onset and progression of autophagy, a protective cellular process...

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Autores principales: Stacchiotti, Alessandra, Rovetta, Francesca, Ferroni, Matteo, Corsetti, Giovanni, Lavazza, Antonio, Sberveglieri, Giorgio, Aleo, Maria Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053152/
https://www.ncbi.nlm.nih.gov/pubmed/24955211
http://dx.doi.org/10.1155/2014/840951
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author Stacchiotti, Alessandra
Rovetta, Francesca
Ferroni, Matteo
Corsetti, Giovanni
Lavazza, Antonio
Sberveglieri, Giorgio
Aleo, Maria Francesca
author_facet Stacchiotti, Alessandra
Rovetta, Francesca
Ferroni, Matteo
Corsetti, Giovanni
Lavazza, Antonio
Sberveglieri, Giorgio
Aleo, Maria Francesca
author_sort Stacchiotti, Alessandra
collection PubMed
description Cisplatin (CisPt) is a widely used chemotherapeutic drug whose side effects include muscle weakness and cachexia. Here we analysed CisPt-induced atrophy in C2C12 myotubes by a multidisciplinary morphological approach, focusing on the onset and progression of autophagy, a protective cellular process that, when excessively activated, may trigger protein hypercatabolism and atrophy in skeletal muscle. To visualize autophagy we used confocal and transmission electron microscopy at different times of treatment and doses of CisPt. Moreover we evaluated the effects of taurine, a cytoprotective beta-amino acid able to counteract oxidative stress, apoptosis, and endoplasmic reticulum stress in different tissues and organs. Our microscopic results indicate that autophagy occurs very early in 50 μM CisPt challenged myotubes (4 h–8 h) before overt atrophy but it persists even at 24 h, when several autophagic vesicles, damaged mitochondria, and sarcoplasmic blebbings engulf the sarcoplasm. Differently, 25 mM taurine pretreatment rescues the majority of myotubes size upon 50 μM CisPt at 24 h. Taurine appears to counteract atrophy by restoring regular microtubular apparatus and mitochondria and reducing the overload and the localization of autophagolysosomes. Such a promising taurine action in preventing atrophy needs further molecular and biochemical studies to best define its impact on muscle homeostasis and the maintenance of an adequate skeletal mass in vivo.
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spelling pubmed-40531522014-06-22 Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study Stacchiotti, Alessandra Rovetta, Francesca Ferroni, Matteo Corsetti, Giovanni Lavazza, Antonio Sberveglieri, Giorgio Aleo, Maria Francesca Oxid Med Cell Longev Research Article Cisplatin (CisPt) is a widely used chemotherapeutic drug whose side effects include muscle weakness and cachexia. Here we analysed CisPt-induced atrophy in C2C12 myotubes by a multidisciplinary morphological approach, focusing on the onset and progression of autophagy, a protective cellular process that, when excessively activated, may trigger protein hypercatabolism and atrophy in skeletal muscle. To visualize autophagy we used confocal and transmission electron microscopy at different times of treatment and doses of CisPt. Moreover we evaluated the effects of taurine, a cytoprotective beta-amino acid able to counteract oxidative stress, apoptosis, and endoplasmic reticulum stress in different tissues and organs. Our microscopic results indicate that autophagy occurs very early in 50 μM CisPt challenged myotubes (4 h–8 h) before overt atrophy but it persists even at 24 h, when several autophagic vesicles, damaged mitochondria, and sarcoplasmic blebbings engulf the sarcoplasm. Differently, 25 mM taurine pretreatment rescues the majority of myotubes size upon 50 μM CisPt at 24 h. Taurine appears to counteract atrophy by restoring regular microtubular apparatus and mitochondria and reducing the overload and the localization of autophagolysosomes. Such a promising taurine action in preventing atrophy needs further molecular and biochemical studies to best define its impact on muscle homeostasis and the maintenance of an adequate skeletal mass in vivo. Hindawi Publishing Corporation 2014 2014-05-13 /pmc/articles/PMC4053152/ /pubmed/24955211 http://dx.doi.org/10.1155/2014/840951 Text en Copyright © 2014 Alessandra Stacchiotti et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Stacchiotti, Alessandra
Rovetta, Francesca
Ferroni, Matteo
Corsetti, Giovanni
Lavazza, Antonio
Sberveglieri, Giorgio
Aleo, Maria Francesca
Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study
title Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study
title_full Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study
title_fullStr Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study
title_full_unstemmed Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study
title_short Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study
title_sort taurine rescues cisplatin-induced muscle atrophy in vitro: a morphological study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053152/
https://www.ncbi.nlm.nih.gov/pubmed/24955211
http://dx.doi.org/10.1155/2014/840951
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