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Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia

Dysregulation of microRNA (miRNA) expression contributes to the pathogenesis of several clinical conditions. The aim of this study is to evaluate the associations between miRNAs and childhood acute lymphoblastic leukemia (ALL) to discover their role in the course of the disease. Forty-three children...

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Autores principales: Duyu, Muhterem, Durmaz, Burak, Gunduz, Cumhur, Vergin, Canan, Yilmaz Karapinar, Deniz, Aksoylar, Serap, Kavakli, Kaan, Cetingul, Nazan, Irken, Gulersu, Yaman, Yontem, Ozkinay, Ferda, Cogulu, Ozgur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053274/
https://www.ncbi.nlm.nih.gov/pubmed/24955371
http://dx.doi.org/10.1155/2014/967585
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author Duyu, Muhterem
Durmaz, Burak
Gunduz, Cumhur
Vergin, Canan
Yilmaz Karapinar, Deniz
Aksoylar, Serap
Kavakli, Kaan
Cetingul, Nazan
Irken, Gulersu
Yaman, Yontem
Ozkinay, Ferda
Cogulu, Ozgur
author_facet Duyu, Muhterem
Durmaz, Burak
Gunduz, Cumhur
Vergin, Canan
Yilmaz Karapinar, Deniz
Aksoylar, Serap
Kavakli, Kaan
Cetingul, Nazan
Irken, Gulersu
Yaman, Yontem
Ozkinay, Ferda
Cogulu, Ozgur
author_sort Duyu, Muhterem
collection PubMed
description Dysregulation of microRNA (miRNA) expression contributes to the pathogenesis of several clinical conditions. The aim of this study is to evaluate the associations between miRNAs and childhood acute lymphoblastic leukemia (ALL) to discover their role in the course of the disease. Forty-three children with ALL and 14 age-matched healthy controls were included in the study. MicroRNA microarray expression profiling was used for peripheral blood and bone marrow samples. Aberrant miRNA expressions associated with the diagnosis and outcome were prospectively evaluated. Confirmation analysis was performed by real time RT-PCR. miR-128, miR-146a, miR-155, miR-181a, and miR-195 were significantly dysregulated in ALL patients at day 0. Following a six-month treatment period, the change in miRNA levels was determined by real time RT-PCR and expression of miR-146a, miR-155, miR-181a, and miR-195 significantly decreased. To conclude, these miRNAs not only may be used as biomarkers in diagnosis of ALL and monitoring the disease but also provide new insights into the potential roles of them in leukemogenesis.
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spelling pubmed-40532742014-06-22 Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia Duyu, Muhterem Durmaz, Burak Gunduz, Cumhur Vergin, Canan Yilmaz Karapinar, Deniz Aksoylar, Serap Kavakli, Kaan Cetingul, Nazan Irken, Gulersu Yaman, Yontem Ozkinay, Ferda Cogulu, Ozgur Biomed Res Int Clinical Study Dysregulation of microRNA (miRNA) expression contributes to the pathogenesis of several clinical conditions. The aim of this study is to evaluate the associations between miRNAs and childhood acute lymphoblastic leukemia (ALL) to discover their role in the course of the disease. Forty-three children with ALL and 14 age-matched healthy controls were included in the study. MicroRNA microarray expression profiling was used for peripheral blood and bone marrow samples. Aberrant miRNA expressions associated with the diagnosis and outcome were prospectively evaluated. Confirmation analysis was performed by real time RT-PCR. miR-128, miR-146a, miR-155, miR-181a, and miR-195 were significantly dysregulated in ALL patients at day 0. Following a six-month treatment period, the change in miRNA levels was determined by real time RT-PCR and expression of miR-146a, miR-155, miR-181a, and miR-195 significantly decreased. To conclude, these miRNAs not only may be used as biomarkers in diagnosis of ALL and monitoring the disease but also provide new insights into the potential roles of them in leukemogenesis. Hindawi Publishing Corporation 2014 2014-05-14 /pmc/articles/PMC4053274/ /pubmed/24955371 http://dx.doi.org/10.1155/2014/967585 Text en Copyright © 2014 Muhterem Duyu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Study
Duyu, Muhterem
Durmaz, Burak
Gunduz, Cumhur
Vergin, Canan
Yilmaz Karapinar, Deniz
Aksoylar, Serap
Kavakli, Kaan
Cetingul, Nazan
Irken, Gulersu
Yaman, Yontem
Ozkinay, Ferda
Cogulu, Ozgur
Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia
title Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia
title_full Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia
title_fullStr Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia
title_full_unstemmed Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia
title_short Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia
title_sort prospective evaluation of whole genome microrna expression profiling in childhood acute lymphoblastic leukemia
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053274/
https://www.ncbi.nlm.nih.gov/pubmed/24955371
http://dx.doi.org/10.1155/2014/967585
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