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Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms

BACKGROUND: Modern automated haematology instruments are capable of performing leukocyte differentials faster, cheaper and with a higher precision than the traditional 100-cell manual differential count. Thus, in human laboratories, criteria are defined for performing a manual review of the blood sm...

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Autores principales: Stirn, Martina, Moritz, Andreas, Bauer, Natali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053280/
https://www.ncbi.nlm.nih.gov/pubmed/24903909
http://dx.doi.org/10.1186/1746-6148-10-125
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author Stirn, Martina
Moritz, Andreas
Bauer, Natali
author_facet Stirn, Martina
Moritz, Andreas
Bauer, Natali
author_sort Stirn, Martina
collection PubMed
description BACKGROUND: Modern automated haematology instruments are capable of performing leukocyte differentials faster, cheaper and with a higher precision than the traditional 100-cell manual differential count. Thus, in human laboratories, criteria are defined for performing a manual review of the blood smear resulting in a marked reduction of manual differential counts. While common in human laboratories, this approach to reducing the number of manual differentials in veterinary laboratories is still not commonly performed. Thus, our aim was to determine the rate and causes of manual leukocyte differentials in a university clinical pathology laboratory using the automated laser-based haematology analyser ADVIA 120. Overall, 14,953 complete blood cell counts from dogs, cats and horses were reviewed. Manual leukocyte differentials were requested if abnormal ADVIA peroxidase and baso cytograms were detected (i.e. suspicion of left shift or atypical lymphocytes/blasts, inappropriate separation of cell populations). RESULTS: In 21% of canine, 32% of feline and 20% of equine samples, a manual differential was requested. Indistinct separation of the cell population was present in 10% to 15% of the cases. Depending on the species, atypical lymphocytes were suspected in 2% to 12%, left shift in 13% to 25% and suspicion of blasts was present in less than 0.4% of the cases. CONCLUSIONS: The obtained results are comparable to those published for human medicine and the rate of manual differentiation could be markedly reduced in veterinary laboratories if microscopic examination was used as a validation procedure rather than as a reflexive substitute for automated differentiation.
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spelling pubmed-40532802014-06-12 Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms Stirn, Martina Moritz, Andreas Bauer, Natali BMC Vet Res Research Article BACKGROUND: Modern automated haematology instruments are capable of performing leukocyte differentials faster, cheaper and with a higher precision than the traditional 100-cell manual differential count. Thus, in human laboratories, criteria are defined for performing a manual review of the blood smear resulting in a marked reduction of manual differential counts. While common in human laboratories, this approach to reducing the number of manual differentials in veterinary laboratories is still not commonly performed. Thus, our aim was to determine the rate and causes of manual leukocyte differentials in a university clinical pathology laboratory using the automated laser-based haematology analyser ADVIA 120. Overall, 14,953 complete blood cell counts from dogs, cats and horses were reviewed. Manual leukocyte differentials were requested if abnormal ADVIA peroxidase and baso cytograms were detected (i.e. suspicion of left shift or atypical lymphocytes/blasts, inappropriate separation of cell populations). RESULTS: In 21% of canine, 32% of feline and 20% of equine samples, a manual differential was requested. Indistinct separation of the cell population was present in 10% to 15% of the cases. Depending on the species, atypical lymphocytes were suspected in 2% to 12%, left shift in 13% to 25% and suspicion of blasts was present in less than 0.4% of the cases. CONCLUSIONS: The obtained results are comparable to those published for human medicine and the rate of manual differentiation could be markedly reduced in veterinary laboratories if microscopic examination was used as a validation procedure rather than as a reflexive substitute for automated differentiation. BioMed Central 2014-06-05 /pmc/articles/PMC4053280/ /pubmed/24903909 http://dx.doi.org/10.1186/1746-6148-10-125 Text en Copyright © 2014 Stirn et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Stirn, Martina
Moritz, Andreas
Bauer, Natali
Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms
title Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms
title_full Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms
title_fullStr Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms
title_full_unstemmed Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms
title_short Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms
title_sort rate of manual leukocyte differentials in dog, cat and horse blood samples using advia 120 cytograms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053280/
https://www.ncbi.nlm.nih.gov/pubmed/24903909
http://dx.doi.org/10.1186/1746-6148-10-125
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