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Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice

Gallic acid (GA), a naturally abundant plant phenolic compound in vegetables and fruits, has been shown to have potent anti-oxidative and anti-obesity activity. However, the effects of GA on nonalcoholic fatty liver disease (NAFLD) are poorly understood. In this study, we investigated the beneficial...

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Autores principales: Chao, Jung, Huo, Teh-Ia, Cheng, Hao-Yuan, Tsai, Jen-Chieh, Liao, Jiunn-Wang, Lee, Meng-Shiou, Qin, Xue-Mei, Hsieh, Ming-Tsuen, Pao, Li-Heng, Peng, Wen-Huang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053315/
https://www.ncbi.nlm.nih.gov/pubmed/24918580
http://dx.doi.org/10.1371/journal.pone.0096969
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author Chao, Jung
Huo, Teh-Ia
Cheng, Hao-Yuan
Tsai, Jen-Chieh
Liao, Jiunn-Wang
Lee, Meng-Shiou
Qin, Xue-Mei
Hsieh, Ming-Tsuen
Pao, Li-Heng
Peng, Wen-Huang
author_facet Chao, Jung
Huo, Teh-Ia
Cheng, Hao-Yuan
Tsai, Jen-Chieh
Liao, Jiunn-Wang
Lee, Meng-Shiou
Qin, Xue-Mei
Hsieh, Ming-Tsuen
Pao, Li-Heng
Peng, Wen-Huang
author_sort Chao, Jung
collection PubMed
description Gallic acid (GA), a naturally abundant plant phenolic compound in vegetables and fruits, has been shown to have potent anti-oxidative and anti-obesity activity. However, the effects of GA on nonalcoholic fatty liver disease (NAFLD) are poorly understood. In this study, we investigated the beneficial effects of GA administration on nutritional hepatosteatosis model by a more “holistic view” approach, namely (1)H NMR-based metabolomics, in order to prove efficacy and to obtain information that might lead to a better understanding of the mode of action of GA. Male C57BL/6 mice were placed for 16 weeks on either a normal chow diet, a high fat diet (HFD, 60%), or a high fat diet supplemented with GA (50 and 100 mg/kg/day, orally). Liver histopathology and serum biochemical examinations indicated that the daily administration of GA protects against hepatic steatosis, obesity, hypercholesterolemia, and insulin resistance among the HFD-induced NAFLD mice. In addition, partial least squares discriminant analysis scores plots demonstrated that the cluster of HFD fed mice is clearly separated from the normal group mice plots, indicating that the metabolic characteristics of these two groups are distinctively different. Specifically, the GA-treated mice are located closer to the normal group of mice, indicating that the HFD-induced disturbances to the metabolic profile were partially reversed by GA treatment. Our results show that the hepatoprotective effect of GA occurs in part through a reversing of the HFD caused disturbances to a range of metabolic pathways, including lipid metabolism, glucose metabolism (glycolysis and gluconeogenesis), amino acids metabolism, choline metabolism and gut-microbiota-associated metabolism. Taken together, this study suggested that a (1)H NMR-based metabolomics approach is a useful platform for natural product functional evaluation. The selected metabolites are potentially useful as preventive action biomarkers and could also be used to help our further understanding of the effect of GA in hepatosteatosis mice.
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spelling pubmed-40533152014-06-18 Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice Chao, Jung Huo, Teh-Ia Cheng, Hao-Yuan Tsai, Jen-Chieh Liao, Jiunn-Wang Lee, Meng-Shiou Qin, Xue-Mei Hsieh, Ming-Tsuen Pao, Li-Heng Peng, Wen-Huang PLoS One Research Article Gallic acid (GA), a naturally abundant plant phenolic compound in vegetables and fruits, has been shown to have potent anti-oxidative and anti-obesity activity. However, the effects of GA on nonalcoholic fatty liver disease (NAFLD) are poorly understood. In this study, we investigated the beneficial effects of GA administration on nutritional hepatosteatosis model by a more “holistic view” approach, namely (1)H NMR-based metabolomics, in order to prove efficacy and to obtain information that might lead to a better understanding of the mode of action of GA. Male C57BL/6 mice were placed for 16 weeks on either a normal chow diet, a high fat diet (HFD, 60%), or a high fat diet supplemented with GA (50 and 100 mg/kg/day, orally). Liver histopathology and serum biochemical examinations indicated that the daily administration of GA protects against hepatic steatosis, obesity, hypercholesterolemia, and insulin resistance among the HFD-induced NAFLD mice. In addition, partial least squares discriminant analysis scores plots demonstrated that the cluster of HFD fed mice is clearly separated from the normal group mice plots, indicating that the metabolic characteristics of these two groups are distinctively different. Specifically, the GA-treated mice are located closer to the normal group of mice, indicating that the HFD-induced disturbances to the metabolic profile were partially reversed by GA treatment. Our results show that the hepatoprotective effect of GA occurs in part through a reversing of the HFD caused disturbances to a range of metabolic pathways, including lipid metabolism, glucose metabolism (glycolysis and gluconeogenesis), amino acids metabolism, choline metabolism and gut-microbiota-associated metabolism. Taken together, this study suggested that a (1)H NMR-based metabolomics approach is a useful platform for natural product functional evaluation. The selected metabolites are potentially useful as preventive action biomarkers and could also be used to help our further understanding of the effect of GA in hepatosteatosis mice. Public Library of Science 2014-06-11 /pmc/articles/PMC4053315/ /pubmed/24918580 http://dx.doi.org/10.1371/journal.pone.0096969 Text en © 2014 Chao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chao, Jung
Huo, Teh-Ia
Cheng, Hao-Yuan
Tsai, Jen-Chieh
Liao, Jiunn-Wang
Lee, Meng-Shiou
Qin, Xue-Mei
Hsieh, Ming-Tsuen
Pao, Li-Heng
Peng, Wen-Huang
Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice
title Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice
title_full Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice
title_fullStr Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice
title_full_unstemmed Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice
title_short Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice
title_sort gallic acid ameliorated impaired glucose and lipid homeostasis in high fat diet-induced nafld mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053315/
https://www.ncbi.nlm.nih.gov/pubmed/24918580
http://dx.doi.org/10.1371/journal.pone.0096969
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