Cargando…
The Real-Time Dynamic Monitoring of microRNA Function in Cholangiocarcinoma
BACKGROUND: Although many studies have confirmed a relationship between microRNAs (miRNAs) and cholangiocarcinoma (CCA), the real-time dynamics of miRNA function have not been examined. METHODS: miRNA reporter constructs were generated using a recombinant adeno-associated virus vector, which contain...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053425/ https://www.ncbi.nlm.nih.gov/pubmed/24918778 http://dx.doi.org/10.1371/journal.pone.0099431 |
_version_ | 1782320379355725824 |
---|---|
author | Chen, Xue Chen, Jing Liu, Xinjuan Guo, Zihao Sun, Xiaoxin Zhang, Jie |
author_facet | Chen, Xue Chen, Jing Liu, Xinjuan Guo, Zihao Sun, Xiaoxin Zhang, Jie |
author_sort | Chen, Xue |
collection | PubMed |
description | BACKGROUND: Although many studies have confirmed a relationship between microRNAs (miRNAs) and cholangiocarcinoma (CCA), the real-time dynamics of miRNA function have not been examined. METHODS: miRNA reporter constructs were generated using a recombinant adeno-associated virus vector, which contained complementary sequences for six miRNAs (miR-200a, miR-200b, miR-21, miR-146a, miR-155, and miR-221), along with two independent expression cassettes encoding the fluorescent reporter genes Fluc and Gluc. The spatio-temporal function of each miRNA was monitored both in CCA and control tissues. RESULTS: All miRNAs participated in CCA development, with distinct patterns of expression over time. The activity of miR-21 was significantly lower in female T3N0M0 CCA tissue relative to controls at three time points, yet was higher in two male T3N1M0 CCA tissues. The difference in miR-200b function between two male T3N1M0 CCA tissues and their corresponding controls peaked at 24 h, while function in a female T3N0M0 CCA was detected only at 72 h. The four remaining miRNAs (miR-200a, miR146a, miR-155, and miR-221) displayed patient-specific activity patterns in both CCA and control tissues. CONCLUSION: Significant variability was observed in the temporal function of all six miRNAs, which may play an important role in the development of CCA. |
format | Online Article Text |
id | pubmed-4053425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40534252014-06-18 The Real-Time Dynamic Monitoring of microRNA Function in Cholangiocarcinoma Chen, Xue Chen, Jing Liu, Xinjuan Guo, Zihao Sun, Xiaoxin Zhang, Jie PLoS One Research Article BACKGROUND: Although many studies have confirmed a relationship between microRNAs (miRNAs) and cholangiocarcinoma (CCA), the real-time dynamics of miRNA function have not been examined. METHODS: miRNA reporter constructs were generated using a recombinant adeno-associated virus vector, which contained complementary sequences for six miRNAs (miR-200a, miR-200b, miR-21, miR-146a, miR-155, and miR-221), along with two independent expression cassettes encoding the fluorescent reporter genes Fluc and Gluc. The spatio-temporal function of each miRNA was monitored both in CCA and control tissues. RESULTS: All miRNAs participated in CCA development, with distinct patterns of expression over time. The activity of miR-21 was significantly lower in female T3N0M0 CCA tissue relative to controls at three time points, yet was higher in two male T3N1M0 CCA tissues. The difference in miR-200b function between two male T3N1M0 CCA tissues and their corresponding controls peaked at 24 h, while function in a female T3N0M0 CCA was detected only at 72 h. The four remaining miRNAs (miR-200a, miR146a, miR-155, and miR-221) displayed patient-specific activity patterns in both CCA and control tissues. CONCLUSION: Significant variability was observed in the temporal function of all six miRNAs, which may play an important role in the development of CCA. Public Library of Science 2014-06-11 /pmc/articles/PMC4053425/ /pubmed/24918778 http://dx.doi.org/10.1371/journal.pone.0099431 Text en © 2014 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chen, Xue Chen, Jing Liu, Xinjuan Guo, Zihao Sun, Xiaoxin Zhang, Jie The Real-Time Dynamic Monitoring of microRNA Function in Cholangiocarcinoma |
title | The Real-Time Dynamic Monitoring of microRNA Function in Cholangiocarcinoma |
title_full | The Real-Time Dynamic Monitoring of microRNA Function in Cholangiocarcinoma |
title_fullStr | The Real-Time Dynamic Monitoring of microRNA Function in Cholangiocarcinoma |
title_full_unstemmed | The Real-Time Dynamic Monitoring of microRNA Function in Cholangiocarcinoma |
title_short | The Real-Time Dynamic Monitoring of microRNA Function in Cholangiocarcinoma |
title_sort | real-time dynamic monitoring of microrna function in cholangiocarcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053425/ https://www.ncbi.nlm.nih.gov/pubmed/24918778 http://dx.doi.org/10.1371/journal.pone.0099431 |
work_keys_str_mv | AT chenxue therealtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma AT chenjing therealtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma AT liuxinjuan therealtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma AT guozihao therealtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma AT sunxiaoxin therealtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma AT zhangjie therealtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma AT chenxue realtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma AT chenjing realtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma AT liuxinjuan realtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma AT guozihao realtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma AT sunxiaoxin realtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma AT zhangjie realtimedynamicmonitoringofmicrornafunctionincholangiocarcinoma |