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PIK3CA and TP53 Gene Mutations in Human Breast Cancer Tumors Frequently Detected by Ion Torrent DNA Sequencing
Breast cancer is the most common malignancy and the leading cause of cancer deaths in women worldwide. While specific genetic mutations have been linked to 5–10% of breast cancer cases, other environmental and epigenetic factors influence the development and progression of the cancer. Since unique m...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053449/ https://www.ncbi.nlm.nih.gov/pubmed/24918944 http://dx.doi.org/10.1371/journal.pone.0099306 |
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author | Bai, Xusheng Zhang, Enke Ye, Hua Nandakumar, Vijayalakshmi Wang, Zhuo Chen, Lihong Tang, Chuanning Li, Jianhui Li, Huijin Zhang, Wei Han, Wei Lou, Feng Zhang, Dandan Sun, Hong Dong, Haichao Zhang, Guangchun Liu, Zhiyuan Dong, Zhishou Guo, Baishuai Yan, He Yan, Chaowei Wang, Lu Su, Ziyi Li, Yangyang Jones, Lindsey Huang, Xue F. Chen, Si-Yi Gao, Jinglong |
author_facet | Bai, Xusheng Zhang, Enke Ye, Hua Nandakumar, Vijayalakshmi Wang, Zhuo Chen, Lihong Tang, Chuanning Li, Jianhui Li, Huijin Zhang, Wei Han, Wei Lou, Feng Zhang, Dandan Sun, Hong Dong, Haichao Zhang, Guangchun Liu, Zhiyuan Dong, Zhishou Guo, Baishuai Yan, He Yan, Chaowei Wang, Lu Su, Ziyi Li, Yangyang Jones, Lindsey Huang, Xue F. Chen, Si-Yi Gao, Jinglong |
author_sort | Bai, Xusheng |
collection | PubMed |
description | Breast cancer is the most common malignancy and the leading cause of cancer deaths in women worldwide. While specific genetic mutations have been linked to 5–10% of breast cancer cases, other environmental and epigenetic factors influence the development and progression of the cancer. Since unique mutations patterns have been observed in individual cancer samples, identification and characterization of the distinctive breast cancer molecular profile is needed to develop more effective target therapies. Until recently, identifying genetic cancer mutations via personalized DNA sequencing was impractical and expensive. The recent technological advancements in next-generation DNA sequencing, such as the semiconductor-based Ion Torrent sequencing platform, has made DNA sequencing cost and time effective with more reliable results. Using the Ion Torrent Ampliseq Cancer Panel, we sequenced 737 loci from 45 cancer-related genes to identify genetic mutations in 105 human breast cancer samples. The sequencing analysis revealed missense mutations in PIK3CA, and TP53 genes in the breast cancer samples of various histologic types. Thus, this study demonstrates the necessity of sequencing individual human cancers in order to develop personalized drugs or combination therapies to effectively target individual, breast cancer-specific mutations. |
format | Online Article Text |
id | pubmed-4053449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40534492014-06-18 PIK3CA and TP53 Gene Mutations in Human Breast Cancer Tumors Frequently Detected by Ion Torrent DNA Sequencing Bai, Xusheng Zhang, Enke Ye, Hua Nandakumar, Vijayalakshmi Wang, Zhuo Chen, Lihong Tang, Chuanning Li, Jianhui Li, Huijin Zhang, Wei Han, Wei Lou, Feng Zhang, Dandan Sun, Hong Dong, Haichao Zhang, Guangchun Liu, Zhiyuan Dong, Zhishou Guo, Baishuai Yan, He Yan, Chaowei Wang, Lu Su, Ziyi Li, Yangyang Jones, Lindsey Huang, Xue F. Chen, Si-Yi Gao, Jinglong PLoS One Research Article Breast cancer is the most common malignancy and the leading cause of cancer deaths in women worldwide. While specific genetic mutations have been linked to 5–10% of breast cancer cases, other environmental and epigenetic factors influence the development and progression of the cancer. Since unique mutations patterns have been observed in individual cancer samples, identification and characterization of the distinctive breast cancer molecular profile is needed to develop more effective target therapies. Until recently, identifying genetic cancer mutations via personalized DNA sequencing was impractical and expensive. The recent technological advancements in next-generation DNA sequencing, such as the semiconductor-based Ion Torrent sequencing platform, has made DNA sequencing cost and time effective with more reliable results. Using the Ion Torrent Ampliseq Cancer Panel, we sequenced 737 loci from 45 cancer-related genes to identify genetic mutations in 105 human breast cancer samples. The sequencing analysis revealed missense mutations in PIK3CA, and TP53 genes in the breast cancer samples of various histologic types. Thus, this study demonstrates the necessity of sequencing individual human cancers in order to develop personalized drugs or combination therapies to effectively target individual, breast cancer-specific mutations. Public Library of Science 2014-06-11 /pmc/articles/PMC4053449/ /pubmed/24918944 http://dx.doi.org/10.1371/journal.pone.0099306 Text en © 2014 Bai et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bai, Xusheng Zhang, Enke Ye, Hua Nandakumar, Vijayalakshmi Wang, Zhuo Chen, Lihong Tang, Chuanning Li, Jianhui Li, Huijin Zhang, Wei Han, Wei Lou, Feng Zhang, Dandan Sun, Hong Dong, Haichao Zhang, Guangchun Liu, Zhiyuan Dong, Zhishou Guo, Baishuai Yan, He Yan, Chaowei Wang, Lu Su, Ziyi Li, Yangyang Jones, Lindsey Huang, Xue F. Chen, Si-Yi Gao, Jinglong PIK3CA and TP53 Gene Mutations in Human Breast Cancer Tumors Frequently Detected by Ion Torrent DNA Sequencing |
title | PIK3CA and TP53 Gene Mutations in Human Breast Cancer Tumors Frequently Detected by Ion Torrent DNA Sequencing |
title_full | PIK3CA and TP53 Gene Mutations in Human Breast Cancer Tumors Frequently Detected by Ion Torrent DNA Sequencing |
title_fullStr | PIK3CA and TP53 Gene Mutations in Human Breast Cancer Tumors Frequently Detected by Ion Torrent DNA Sequencing |
title_full_unstemmed | PIK3CA and TP53 Gene Mutations in Human Breast Cancer Tumors Frequently Detected by Ion Torrent DNA Sequencing |
title_short | PIK3CA and TP53 Gene Mutations in Human Breast Cancer Tumors Frequently Detected by Ion Torrent DNA Sequencing |
title_sort | pik3ca and tp53 gene mutations in human breast cancer tumors frequently detected by ion torrent dna sequencing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053449/ https://www.ncbi.nlm.nih.gov/pubmed/24918944 http://dx.doi.org/10.1371/journal.pone.0099306 |
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