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Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum
BACKGROUND: The post-genomic era of malaria research provided unprecedented insights into the biology of Plasmodium parasites. Due to the large evolutionary distance to model eukaryotes, however, we lack a profound understanding of many processes in Plasmodium biology. One example is the cell nucleu...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053738/ https://www.ncbi.nlm.nih.gov/pubmed/23181666 http://dx.doi.org/10.1186/gb-2012-13-11-r108 |
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author | Oehring, Sophie C Woodcroft, Ben J Moes, Suzette Wetzel, Johanna Dietz, Olivier Pulfer, Andreas Dekiwadia, Chaitali Maeser, Pascal Flueck, Christian Witmer, Kathrin Brancucci, Nicolas MB Niederwieser, Igor Jenoe, Paul Ralph, Stuart A Voss, Till S |
author_facet | Oehring, Sophie C Woodcroft, Ben J Moes, Suzette Wetzel, Johanna Dietz, Olivier Pulfer, Andreas Dekiwadia, Chaitali Maeser, Pascal Flueck, Christian Witmer, Kathrin Brancucci, Nicolas MB Niederwieser, Igor Jenoe, Paul Ralph, Stuart A Voss, Till S |
author_sort | Oehring, Sophie C |
collection | PubMed |
description | BACKGROUND: The post-genomic era of malaria research provided unprecedented insights into the biology of Plasmodium parasites. Due to the large evolutionary distance to model eukaryotes, however, we lack a profound understanding of many processes in Plasmodium biology. One example is the cell nucleus, which controls the parasite genome in a development- and cell cycle-specific manner through mostly unknown mechanisms. To study this important organelle in detail, we conducted an integrative analysis of the P. falciparum nuclear proteome. RESULTS: We combined high accuracy mass spectrometry and bioinformatic approaches to present for the first time an experimentally determined core nuclear proteome for P. falciparum. Besides a large number of factors implicated in known nuclear processes, one-third of all detected proteins carry no functional annotation, including many phylum- or genus-specific factors. Importantly, extensive experimental validation using 30 transgenic cell lines confirmed the high specificity of this inventory, and revealed distinct nuclear localization patterns of hitherto uncharacterized proteins. Further, our detailed analysis identified novel protein domains potentially implicated in gene transcription pathways, and sheds important new light on nuclear compartments and processes including regulatory complexes, the nucleolus, nuclear pores, and nuclear import pathways. CONCLUSION: Our study provides comprehensive new insight into the biology of the Plasmodium nucleus and will serve as an important platform for dissecting general and parasite-specific nuclear processes in malaria parasites. Moreover, as the first nuclear proteome characterized in any protist organism, it will provide an important resource for studying evolutionary aspects of nuclear biology. |
format | Online Article Text |
id | pubmed-4053738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40537382014-06-13 Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum Oehring, Sophie C Woodcroft, Ben J Moes, Suzette Wetzel, Johanna Dietz, Olivier Pulfer, Andreas Dekiwadia, Chaitali Maeser, Pascal Flueck, Christian Witmer, Kathrin Brancucci, Nicolas MB Niederwieser, Igor Jenoe, Paul Ralph, Stuart A Voss, Till S Genome Biol Research BACKGROUND: The post-genomic era of malaria research provided unprecedented insights into the biology of Plasmodium parasites. Due to the large evolutionary distance to model eukaryotes, however, we lack a profound understanding of many processes in Plasmodium biology. One example is the cell nucleus, which controls the parasite genome in a development- and cell cycle-specific manner through mostly unknown mechanisms. To study this important organelle in detail, we conducted an integrative analysis of the P. falciparum nuclear proteome. RESULTS: We combined high accuracy mass spectrometry and bioinformatic approaches to present for the first time an experimentally determined core nuclear proteome for P. falciparum. Besides a large number of factors implicated in known nuclear processes, one-third of all detected proteins carry no functional annotation, including many phylum- or genus-specific factors. Importantly, extensive experimental validation using 30 transgenic cell lines confirmed the high specificity of this inventory, and revealed distinct nuclear localization patterns of hitherto uncharacterized proteins. Further, our detailed analysis identified novel protein domains potentially implicated in gene transcription pathways, and sheds important new light on nuclear compartments and processes including regulatory complexes, the nucleolus, nuclear pores, and nuclear import pathways. CONCLUSION: Our study provides comprehensive new insight into the biology of the Plasmodium nucleus and will serve as an important platform for dissecting general and parasite-specific nuclear processes in malaria parasites. Moreover, as the first nuclear proteome characterized in any protist organism, it will provide an important resource for studying evolutionary aspects of nuclear biology. BioMed Central 2012 2012-11-26 /pmc/articles/PMC4053738/ /pubmed/23181666 http://dx.doi.org/10.1186/gb-2012-13-11-r108 Text en Copyright © 2013 Oehring et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Oehring, Sophie C Woodcroft, Ben J Moes, Suzette Wetzel, Johanna Dietz, Olivier Pulfer, Andreas Dekiwadia, Chaitali Maeser, Pascal Flueck, Christian Witmer, Kathrin Brancucci, Nicolas MB Niederwieser, Igor Jenoe, Paul Ralph, Stuart A Voss, Till S Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum |
title | Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum |
title_full | Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum |
title_fullStr | Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum |
title_full_unstemmed | Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum |
title_short | Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum |
title_sort | organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite plasmodium falciparum |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053738/ https://www.ncbi.nlm.nih.gov/pubmed/23181666 http://dx.doi.org/10.1186/gb-2012-13-11-r108 |
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