Cargando…
AHT-ChIP-seq: a completely automated robotic protocol for high-throughput chromatin immunoprecipitation
ChIP-seq is an established manually-performed method for identifying DNA-protein interactions genome-wide. Here, we describe a protocol for automated high-throughput (AHT) ChIP-seq. To demonstrate the quality of data obtained using AHT-ChIP-seq, we applied it to five proteins in mouse livers using a...
Autores principales: | Aldridge, Sarah, Watt, Stephen, Quail, Michael A, Rayner, Tim, Lukk, Margus, Bimson, Michael F, Gaffney, Daniel, Odom, Duncan T |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053851/ https://www.ncbi.nlm.nih.gov/pubmed/24200198 http://dx.doi.org/10.1186/gb-2013-14-11-r124 |
Ejemplares similares
-
Fully automated high-throughput chromatin immunoprecipitation for ChIP-seq: Identifying ChIP-quality p300 monoclonal antibodies
por: Gasper, William C., et al.
Publicado: (2014) -
A ChIP on the shoulder? Chromatin immunoprecipitation and validation strategies for ChIP antibodies
por: Wardle, Fiona C., et al.
Publicado: (2015) -
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
por: Giaimo, Benedetto Daniele, et al.
Publicado: (2017) -
Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
por: Strenkert, Daniela, et al.
Publicado: (2011) -
Accounting for immunoprecipitation efficiencies in the statistical analysis of ChIP-seq data
por: Bao, Yanchun, et al.
Publicado: (2013)