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Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe

We conducted a cross-sectional study of second line drug resistance patterns and genetic diversity of MDR-TB isolates archived at the BRTI-TB Laboratory, Harare, between January 2007 and December 2011. DSTs were performed for second line antituberculosis drugs. XDR-TB strains were defined as MDR-TB...

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Autores principales: Sagonda, Tichaona, Mupfumi, Lucy, Manzou, Rumbidzai, Makamure, Beauty, Tshabalala, Mqondisi, Gwanzura, Lovemore, Mason, Peter, Mutetwa, Reggie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4054961/
https://www.ncbi.nlm.nih.gov/pubmed/24967101
http://dx.doi.org/10.1155/2014/349141
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author Sagonda, Tichaona
Mupfumi, Lucy
Manzou, Rumbidzai
Makamure, Beauty
Tshabalala, Mqondisi
Gwanzura, Lovemore
Mason, Peter
Mutetwa, Reggie
author_facet Sagonda, Tichaona
Mupfumi, Lucy
Manzou, Rumbidzai
Makamure, Beauty
Tshabalala, Mqondisi
Gwanzura, Lovemore
Mason, Peter
Mutetwa, Reggie
author_sort Sagonda, Tichaona
collection PubMed
description We conducted a cross-sectional study of second line drug resistance patterns and genetic diversity of MDR-TB isolates archived at the BRTI-TB Laboratory, Harare, between January 2007 and December 2011. DSTs were performed for second line antituberculosis drugs. XDR-TB strains were defined as MDR-TB strains with resistance to either kanamycin and ofloxacin or capreomycin and ofloxacin. Strain types were identified by spoligotyping. No resistance to any second line drugs was shown in 73% of the isolates, with 23% resistant to one or two drugs but not meeting the definition of XDR-TB. A total of 26 shared types were identified, and 18 (69%) matched preexisting shared types in the current published spoligotype databases. Of the 11 out of 18 clustered SITs, 4 predominant (>6 isolates per shared type) were identified. The most and least abundant types were SIT 1468 (LAM 11-ZWE) with 12 (18%) isolates and SIT 53 (T1) with 6 (9%) isolates, respectively. XDR-TB strains are rare in Zimbabwe, but the high proportion of “pre-XDR-TB” strains and treatment failure cases is of concern. The genetic diversity of the MDR-TB strains showed no significant association between SITs and drug resistance.
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spelling pubmed-40549612014-06-25 Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe Sagonda, Tichaona Mupfumi, Lucy Manzou, Rumbidzai Makamure, Beauty Tshabalala, Mqondisi Gwanzura, Lovemore Mason, Peter Mutetwa, Reggie Tuberc Res Treat Research Article We conducted a cross-sectional study of second line drug resistance patterns and genetic diversity of MDR-TB isolates archived at the BRTI-TB Laboratory, Harare, between January 2007 and December 2011. DSTs were performed for second line antituberculosis drugs. XDR-TB strains were defined as MDR-TB strains with resistance to either kanamycin and ofloxacin or capreomycin and ofloxacin. Strain types were identified by spoligotyping. No resistance to any second line drugs was shown in 73% of the isolates, with 23% resistant to one or two drugs but not meeting the definition of XDR-TB. A total of 26 shared types were identified, and 18 (69%) matched preexisting shared types in the current published spoligotype databases. Of the 11 out of 18 clustered SITs, 4 predominant (>6 isolates per shared type) were identified. The most and least abundant types were SIT 1468 (LAM 11-ZWE) with 12 (18%) isolates and SIT 53 (T1) with 6 (9%) isolates, respectively. XDR-TB strains are rare in Zimbabwe, but the high proportion of “pre-XDR-TB” strains and treatment failure cases is of concern. The genetic diversity of the MDR-TB strains showed no significant association between SITs and drug resistance. Hindawi Publishing Corporation 2014 2014-05-20 /pmc/articles/PMC4054961/ /pubmed/24967101 http://dx.doi.org/10.1155/2014/349141 Text en Copyright © 2014 Tichaona Sagonda et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sagonda, Tichaona
Mupfumi, Lucy
Manzou, Rumbidzai
Makamure, Beauty
Tshabalala, Mqondisi
Gwanzura, Lovemore
Mason, Peter
Mutetwa, Reggie
Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe
title Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe
title_full Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe
title_fullStr Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe
title_full_unstemmed Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe
title_short Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe
title_sort prevalence of extensively drug resistant tuberculosis among archived multidrug resistant tuberculosis isolates in zimbabwe
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4054961/
https://www.ncbi.nlm.nih.gov/pubmed/24967101
http://dx.doi.org/10.1155/2014/349141
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