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Sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells
INTRODUCTION: Overweight status should not be considered merely an aesthetic concern; rather, it can incur health risks since it may trigger a cascade of events that produce further fat tissue through altered levels of circulating signaling molecules. There have been few studies addressing the effec...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055107/ https://www.ncbi.nlm.nih.gov/pubmed/24405848 http://dx.doi.org/10.1186/scrt393 |
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author | Di Bernardo, Giovanni Messina, Giovanni Capasso, Stefania Del Gaudio, Stefania Cipollaro, Marilena Peluso, Gianfranco Casale, Fiorina Monda, Marcellino Galderisi, Umberto |
author_facet | Di Bernardo, Giovanni Messina, Giovanni Capasso, Stefania Del Gaudio, Stefania Cipollaro, Marilena Peluso, Gianfranco Casale, Fiorina Monda, Marcellino Galderisi, Umberto |
author_sort | Di Bernardo, Giovanni |
collection | PubMed |
description | INTRODUCTION: Overweight status should not be considered merely an aesthetic concern; rather, it can incur health risks since it may trigger a cascade of events that produce further fat tissue through altered levels of circulating signaling molecules. There have been few studies addressing the effect of overweight status on the physiological functions of stem cells, including mesenchymal stem cells (MSCs), which are the progenitors of adipocytes and osteocytes and are a subset of the bone marrow stromal cell population. METHODS: We decided to investigate the influence of overweight individuals’ sera on in vitro MSC proliferation and differentiation. RESULTS: We observed that in vitro incubation of bone marrow stromal cells with the sera of overweight individuals promotes the adipogenic differentiation of MSCs while partially impairing proper osteogenesis. CONCLUSIONS: These results, which represent a pilot study, might suggest that becoming overweight triggers further weight gains by promoting a bias in the differentiation potential of MSCs toward adipogenesis. The circulating factors involved in this phenomenon remain to be determined, since the great majority of the well known pro-inflammatory cytokines and adipocyte-secreted factors we investigated did not show relevant modifications in overweight serum samples compared with controls. |
format | Online Article Text |
id | pubmed-4055107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40551072014-06-15 Sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells Di Bernardo, Giovanni Messina, Giovanni Capasso, Stefania Del Gaudio, Stefania Cipollaro, Marilena Peluso, Gianfranco Casale, Fiorina Monda, Marcellino Galderisi, Umberto Stem Cell Res Ther Research INTRODUCTION: Overweight status should not be considered merely an aesthetic concern; rather, it can incur health risks since it may trigger a cascade of events that produce further fat tissue through altered levels of circulating signaling molecules. There have been few studies addressing the effect of overweight status on the physiological functions of stem cells, including mesenchymal stem cells (MSCs), which are the progenitors of adipocytes and osteocytes and are a subset of the bone marrow stromal cell population. METHODS: We decided to investigate the influence of overweight individuals’ sera on in vitro MSC proliferation and differentiation. RESULTS: We observed that in vitro incubation of bone marrow stromal cells with the sera of overweight individuals promotes the adipogenic differentiation of MSCs while partially impairing proper osteogenesis. CONCLUSIONS: These results, which represent a pilot study, might suggest that becoming overweight triggers further weight gains by promoting a bias in the differentiation potential of MSCs toward adipogenesis. The circulating factors involved in this phenomenon remain to be determined, since the great majority of the well known pro-inflammatory cytokines and adipocyte-secreted factors we investigated did not show relevant modifications in overweight serum samples compared with controls. BioMed Central 2014-01-09 /pmc/articles/PMC4055107/ /pubmed/24405848 http://dx.doi.org/10.1186/scrt393 Text en Copyright © 2014 Di Bernardo et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Di Bernardo, Giovanni Messina, Giovanni Capasso, Stefania Del Gaudio, Stefania Cipollaro, Marilena Peluso, Gianfranco Casale, Fiorina Monda, Marcellino Galderisi, Umberto Sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells |
title | Sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells |
title_full | Sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells |
title_fullStr | Sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells |
title_full_unstemmed | Sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells |
title_short | Sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells |
title_sort | sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055107/ https://www.ncbi.nlm.nih.gov/pubmed/24405848 http://dx.doi.org/10.1186/scrt393 |
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