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Lipid domain–dependent regulation of single-cell wound repair

After damage, cells reseal their plasma membrane and repair the underlying cortical cytoskeleton. Although many different proteins have been implicated in cell repair, the potential role of specific lipids has not been explored. Here we report that cell damage elicits rapid formation of spatially or...

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Autores principales: Vaughan, Emily M., You, Jae-Sung, Elsie Yu, Hoi-Ying, Lasek, Amber, Vitale, Nicolas, Hornberger, Troy A., Bement, William M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055266/
https://www.ncbi.nlm.nih.gov/pubmed/24790096
http://dx.doi.org/10.1091/mbc.E14-03-0839
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author Vaughan, Emily M.
You, Jae-Sung
Elsie Yu, Hoi-Ying
Lasek, Amber
Vitale, Nicolas
Hornberger, Troy A.
Bement, William M.
author_facet Vaughan, Emily M.
You, Jae-Sung
Elsie Yu, Hoi-Ying
Lasek, Amber
Vitale, Nicolas
Hornberger, Troy A.
Bement, William M.
author_sort Vaughan, Emily M.
collection PubMed
description After damage, cells reseal their plasma membrane and repair the underlying cortical cytoskeleton. Although many different proteins have been implicated in cell repair, the potential role of specific lipids has not been explored. Here we report that cell damage elicits rapid formation of spatially organized lipid domains around the damage site, with different lipids concentrated in different domains as a result of both de novo synthesis and transport. One of these lipids—diacylglycerol (DAG)—rapidly accumulates in a broad domain that overlaps the zones of active Rho and Cdc42, GTPases that regulate repair of the cortical cytoskeleton. Formation of the DAG domain is required for Cdc42 and Rho activation and healing. Two DAG targets, protein kinase C (PKC) β and η, are recruited to cell wounds and play mutually antagonistic roles in the healing process: PKCβ participates in Rho and Cdc42 activation, whereas PKCη inhibits Rho and Cdc42 activation. The results reveal an unexpected diversity in subcellular lipid domains and the importance of such domains for a basic cellular process.
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spelling pubmed-40552662014-08-30 Lipid domain–dependent regulation of single-cell wound repair Vaughan, Emily M. You, Jae-Sung Elsie Yu, Hoi-Ying Lasek, Amber Vitale, Nicolas Hornberger, Troy A. Bement, William M. Mol Biol Cell Articles After damage, cells reseal their plasma membrane and repair the underlying cortical cytoskeleton. Although many different proteins have been implicated in cell repair, the potential role of specific lipids has not been explored. Here we report that cell damage elicits rapid formation of spatially organized lipid domains around the damage site, with different lipids concentrated in different domains as a result of both de novo synthesis and transport. One of these lipids—diacylglycerol (DAG)—rapidly accumulates in a broad domain that overlaps the zones of active Rho and Cdc42, GTPases that regulate repair of the cortical cytoskeleton. Formation of the DAG domain is required for Cdc42 and Rho activation and healing. Two DAG targets, protein kinase C (PKC) β and η, are recruited to cell wounds and play mutually antagonistic roles in the healing process: PKCβ participates in Rho and Cdc42 activation, whereas PKCη inhibits Rho and Cdc42 activation. The results reveal an unexpected diversity in subcellular lipid domains and the importance of such domains for a basic cellular process. The American Society for Cell Biology 2014-06-15 /pmc/articles/PMC4055266/ /pubmed/24790096 http://dx.doi.org/10.1091/mbc.E14-03-0839 Text en © 2014 Vaughan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Vaughan, Emily M.
You, Jae-Sung
Elsie Yu, Hoi-Ying
Lasek, Amber
Vitale, Nicolas
Hornberger, Troy A.
Bement, William M.
Lipid domain–dependent regulation of single-cell wound repair
title Lipid domain–dependent regulation of single-cell wound repair
title_full Lipid domain–dependent regulation of single-cell wound repair
title_fullStr Lipid domain–dependent regulation of single-cell wound repair
title_full_unstemmed Lipid domain–dependent regulation of single-cell wound repair
title_short Lipid domain–dependent regulation of single-cell wound repair
title_sort lipid domain–dependent regulation of single-cell wound repair
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055266/
https://www.ncbi.nlm.nih.gov/pubmed/24790096
http://dx.doi.org/10.1091/mbc.E14-03-0839
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