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Cooperative endocytosis of the endosomal SNARE protein syntaxin-8 and the potassium channel TASK-1

The endosomal SNARE protein syntaxin-8 interacts with the acid-sensitive potassium channel TASK-1. The functional relevance of this interaction was studied by heterologous expression of these proteins (and mutants thereof) in Xenopus oocytes and in mammalian cell lines. Coexpression of syntaxin-8 ca...

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Autores principales: Renigunta, Vijay, Fischer, Thomas, Zuzarte, Marylou, Kling, Stefan, Zou, Xinle, Siebert, Kai, Limberg, Maren M., Rinné, Susanne, Decher, Niels, Schlichthörl, Günter, Daut, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055267/
https://www.ncbi.nlm.nih.gov/pubmed/24743596
http://dx.doi.org/10.1091/mbc.E13-10-0592
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author Renigunta, Vijay
Fischer, Thomas
Zuzarte, Marylou
Kling, Stefan
Zou, Xinle
Siebert, Kai
Limberg, Maren M.
Rinné, Susanne
Decher, Niels
Schlichthörl, Günter
Daut, Jürgen
author_facet Renigunta, Vijay
Fischer, Thomas
Zuzarte, Marylou
Kling, Stefan
Zou, Xinle
Siebert, Kai
Limberg, Maren M.
Rinné, Susanne
Decher, Niels
Schlichthörl, Günter
Daut, Jürgen
author_sort Renigunta, Vijay
collection PubMed
description The endosomal SNARE protein syntaxin-8 interacts with the acid-sensitive potassium channel TASK-1. The functional relevance of this interaction was studied by heterologous expression of these proteins (and mutants thereof) in Xenopus oocytes and in mammalian cell lines. Coexpression of syntaxin-8 caused a fourfold reduction in TASK-1 current, a corresponding reduction in the expression of TASK-1 at the cell surface, and a marked increase in the rate of endocytosis of the channel. TASK-1 and syntaxin-8 colocalized in the early endosomal compartment, as indicated by the endosomal markers 2xFYVE and rab5. The stimulatory effect of the SNARE protein on the endocytosis of the channel was abolished when both an endocytosis signal in TASK-1 and an endocytosis signal in syntaxin-8 were mutated. A syntaxin-8 mutant that cannot assemble with other SNARE proteins had virtually the same effect as wild-type syntaxin-8. Total internal reflection fluorescence microscopy showed formation and endocytosis of vesicles containing fluorescence-tagged clathrin, TASK-1, and/or syntaxin-8. Our results suggest that the unassembled form of syntaxin-8 and the potassium channel TASK-1 are internalized via clathrin-mediated endocytosis in a cooperative manner. This implies that syntaxin-8 regulates the endocytosis of TASK-1. Our study supports the idea that endosomal SNARE proteins can have functions unrelated to membrane fusion.
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spelling pubmed-40552672014-08-30 Cooperative endocytosis of the endosomal SNARE protein syntaxin-8 and the potassium channel TASK-1 Renigunta, Vijay Fischer, Thomas Zuzarte, Marylou Kling, Stefan Zou, Xinle Siebert, Kai Limberg, Maren M. Rinné, Susanne Decher, Niels Schlichthörl, Günter Daut, Jürgen Mol Biol Cell Articles The endosomal SNARE protein syntaxin-8 interacts with the acid-sensitive potassium channel TASK-1. The functional relevance of this interaction was studied by heterologous expression of these proteins (and mutants thereof) in Xenopus oocytes and in mammalian cell lines. Coexpression of syntaxin-8 caused a fourfold reduction in TASK-1 current, a corresponding reduction in the expression of TASK-1 at the cell surface, and a marked increase in the rate of endocytosis of the channel. TASK-1 and syntaxin-8 colocalized in the early endosomal compartment, as indicated by the endosomal markers 2xFYVE and rab5. The stimulatory effect of the SNARE protein on the endocytosis of the channel was abolished when both an endocytosis signal in TASK-1 and an endocytosis signal in syntaxin-8 were mutated. A syntaxin-8 mutant that cannot assemble with other SNARE proteins had virtually the same effect as wild-type syntaxin-8. Total internal reflection fluorescence microscopy showed formation and endocytosis of vesicles containing fluorescence-tagged clathrin, TASK-1, and/or syntaxin-8. Our results suggest that the unassembled form of syntaxin-8 and the potassium channel TASK-1 are internalized via clathrin-mediated endocytosis in a cooperative manner. This implies that syntaxin-8 regulates the endocytosis of TASK-1. Our study supports the idea that endosomal SNARE proteins can have functions unrelated to membrane fusion. The American Society for Cell Biology 2014-06-15 /pmc/articles/PMC4055267/ /pubmed/24743596 http://dx.doi.org/10.1091/mbc.E13-10-0592 Text en © 2014 Renigunta et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Renigunta, Vijay
Fischer, Thomas
Zuzarte, Marylou
Kling, Stefan
Zou, Xinle
Siebert, Kai
Limberg, Maren M.
Rinné, Susanne
Decher, Niels
Schlichthörl, Günter
Daut, Jürgen
Cooperative endocytosis of the endosomal SNARE protein syntaxin-8 and the potassium channel TASK-1
title Cooperative endocytosis of the endosomal SNARE protein syntaxin-8 and the potassium channel TASK-1
title_full Cooperative endocytosis of the endosomal SNARE protein syntaxin-8 and the potassium channel TASK-1
title_fullStr Cooperative endocytosis of the endosomal SNARE protein syntaxin-8 and the potassium channel TASK-1
title_full_unstemmed Cooperative endocytosis of the endosomal SNARE protein syntaxin-8 and the potassium channel TASK-1
title_short Cooperative endocytosis of the endosomal SNARE protein syntaxin-8 and the potassium channel TASK-1
title_sort cooperative endocytosis of the endosomal snare protein syntaxin-8 and the potassium channel task-1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055267/
https://www.ncbi.nlm.nih.gov/pubmed/24743596
http://dx.doi.org/10.1091/mbc.E13-10-0592
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