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Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions

Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer c...

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Autores principales: Milašinović, Nikola, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lučić Škorić, Marija, Filipović, Jovanka, Kalagasidis Krušić, Melina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055544/
https://www.ncbi.nlm.nih.gov/pubmed/24982870
http://dx.doi.org/10.1155/2014/364930
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author Milašinović, Nikola
Knežević-Jugović, Zorica
Milosavljević, Nedeljko
Lučić Škorić, Marija
Filipović, Jovanka
Kalagasidis Krušić, Melina
author_facet Milašinović, Nikola
Knežević-Jugović, Zorica
Milosavljević, Nedeljko
Lučić Škorić, Marija
Filipović, Jovanka
Kalagasidis Krušić, Melina
author_sort Milašinović, Nikola
collection PubMed
description Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.
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spelling pubmed-40555442014-06-30 Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions Milašinović, Nikola Knežević-Jugović, Zorica Milosavljević, Nedeljko Lučić Škorić, Marija Filipović, Jovanka Kalagasidis Krušić, Melina Biomed Res Int Research Article Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well. Hindawi Publishing Corporation 2014 2014-05-25 /pmc/articles/PMC4055544/ /pubmed/24982870 http://dx.doi.org/10.1155/2014/364930 Text en Copyright © 2014 Nikola Milašinović et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Milašinović, Nikola
Knežević-Jugović, Zorica
Milosavljević, Nedeljko
Lučić Škorić, Marija
Filipović, Jovanka
Kalagasidis Krušić, Melina
Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
title Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
title_full Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
title_fullStr Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
title_full_unstemmed Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
title_short Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
title_sort stimuli-sensitive hydrogel based on n-isopropylacrylamide and itaconic acid for entrapment and controlled release of candida rugosa lipase under mild conditions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055544/
https://www.ncbi.nlm.nih.gov/pubmed/24982870
http://dx.doi.org/10.1155/2014/364930
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