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Yi Qi Qing Re Gao Attenuates Podocyte Injury and Inhibits Vascular Endothelial Growth Factor Overexpression in Puromycin Aminonucleoside Rat Model

Proteinuria is the hallmark of chronic kidney disease. Podocyte damage underlies the formation of proteinuria, and vascular endothelial growth factor (VEGF) functions as an autocrine/paracrine regulator. Yi Qi Qing Re Gao (YQQRG) has been used to treat proteinuria for more than two decades. The obje...

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Autores principales: Zhan, Yongli, Yang, Liping, Wen, Yumin, Liu, Huijie, Zhang, Haojun, Zhu, Bin, Han, Wenbing, Gu, Yanting, Sun, Xueyan, Dong, Xi, Zhao, Tingting, Ma, Huixia, Li, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055581/
https://www.ncbi.nlm.nih.gov/pubmed/24963322
http://dx.doi.org/10.1155/2014/375986
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author Zhan, Yongli
Yang, Liping
Wen, Yumin
Liu, Huijie
Zhang, Haojun
Zhu, Bin
Han, Wenbing
Gu, Yanting
Sun, Xueyan
Dong, Xi
Zhao, Tingting
Ma, Huixia
Li, Ping
author_facet Zhan, Yongli
Yang, Liping
Wen, Yumin
Liu, Huijie
Zhang, Haojun
Zhu, Bin
Han, Wenbing
Gu, Yanting
Sun, Xueyan
Dong, Xi
Zhao, Tingting
Ma, Huixia
Li, Ping
author_sort Zhan, Yongli
collection PubMed
description Proteinuria is the hallmark of chronic kidney disease. Podocyte damage underlies the formation of proteinuria, and vascular endothelial growth factor (VEGF) functions as an autocrine/paracrine regulator. Yi Qi Qing Re Gao (YQQRG) has been used to treat proteinuria for more than two decades. The objective of this study was to investigate the protective effect and possible mechanisms of YQQRG on puromycin aminonucleoside (PAN) rat model. Eighty male Sprague-Dawley rats were randomized into sham group, PAN group, PAN + YQQRG group, and PAN + fosinopril group. Treatments were started 7 days before induction of nephrosis (a single intravenous injection of 40 mg/kg PAN) until day 15. 24 h urinary samples were collected on days 5, 9, and 14. The animals were sacrificed on days 3, 10, and 15, respectively. Blood samples and renal tissues were obtained for detection of biochemical and molecular biological parameters. YQQRG significantly reduced proteinuria, elevated serum albumin, and alleviated renal pathological lesions. YQQRG inhibited VEGF-A, nephrin, podocin, and CD2AP mRNA expression and elevated nephrin, podocin, and CD2AP protein levels starting on day 3. In conclusion, YQQRG attenuates podocyte injury in the rat PAN model through downregulation of VEGF-A and restoration of nephrin, podocin, and CD2AP protein expression.
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spelling pubmed-40555812014-06-24 Yi Qi Qing Re Gao Attenuates Podocyte Injury and Inhibits Vascular Endothelial Growth Factor Overexpression in Puromycin Aminonucleoside Rat Model Zhan, Yongli Yang, Liping Wen, Yumin Liu, Huijie Zhang, Haojun Zhu, Bin Han, Wenbing Gu, Yanting Sun, Xueyan Dong, Xi Zhao, Tingting Ma, Huixia Li, Ping Evid Based Complement Alternat Med Research Article Proteinuria is the hallmark of chronic kidney disease. Podocyte damage underlies the formation of proteinuria, and vascular endothelial growth factor (VEGF) functions as an autocrine/paracrine regulator. Yi Qi Qing Re Gao (YQQRG) has been used to treat proteinuria for more than two decades. The objective of this study was to investigate the protective effect and possible mechanisms of YQQRG on puromycin aminonucleoside (PAN) rat model. Eighty male Sprague-Dawley rats were randomized into sham group, PAN group, PAN + YQQRG group, and PAN + fosinopril group. Treatments were started 7 days before induction of nephrosis (a single intravenous injection of 40 mg/kg PAN) until day 15. 24 h urinary samples were collected on days 5, 9, and 14. The animals were sacrificed on days 3, 10, and 15, respectively. Blood samples and renal tissues were obtained for detection of biochemical and molecular biological parameters. YQQRG significantly reduced proteinuria, elevated serum albumin, and alleviated renal pathological lesions. YQQRG inhibited VEGF-A, nephrin, podocin, and CD2AP mRNA expression and elevated nephrin, podocin, and CD2AP protein levels starting on day 3. In conclusion, YQQRG attenuates podocyte injury in the rat PAN model through downregulation of VEGF-A and restoration of nephrin, podocin, and CD2AP protein expression. Hindawi Publishing Corporation 2014 2014-05-21 /pmc/articles/PMC4055581/ /pubmed/24963322 http://dx.doi.org/10.1155/2014/375986 Text en Copyright © 2014 Yongli Zhan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhan, Yongli
Yang, Liping
Wen, Yumin
Liu, Huijie
Zhang, Haojun
Zhu, Bin
Han, Wenbing
Gu, Yanting
Sun, Xueyan
Dong, Xi
Zhao, Tingting
Ma, Huixia
Li, Ping
Yi Qi Qing Re Gao Attenuates Podocyte Injury and Inhibits Vascular Endothelial Growth Factor Overexpression in Puromycin Aminonucleoside Rat Model
title Yi Qi Qing Re Gao Attenuates Podocyte Injury and Inhibits Vascular Endothelial Growth Factor Overexpression in Puromycin Aminonucleoside Rat Model
title_full Yi Qi Qing Re Gao Attenuates Podocyte Injury and Inhibits Vascular Endothelial Growth Factor Overexpression in Puromycin Aminonucleoside Rat Model
title_fullStr Yi Qi Qing Re Gao Attenuates Podocyte Injury and Inhibits Vascular Endothelial Growth Factor Overexpression in Puromycin Aminonucleoside Rat Model
title_full_unstemmed Yi Qi Qing Re Gao Attenuates Podocyte Injury and Inhibits Vascular Endothelial Growth Factor Overexpression in Puromycin Aminonucleoside Rat Model
title_short Yi Qi Qing Re Gao Attenuates Podocyte Injury and Inhibits Vascular Endothelial Growth Factor Overexpression in Puromycin Aminonucleoside Rat Model
title_sort yi qi qing re gao attenuates podocyte injury and inhibits vascular endothelial growth factor overexpression in puromycin aminonucleoside rat model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055581/
https://www.ncbi.nlm.nih.gov/pubmed/24963322
http://dx.doi.org/10.1155/2014/375986
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