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Real-Time Dopamine Measurement in Awake Monkeys

Fast-scan cyclic voltammetry (FSCV) is often used to measure real-time dopamine (DA) concentrations in awake, behaving rodents. Extending this technique to work in monkeys would provide a platform for advanced behavioral studies and a primate model for preclinical research. The present study demonst...

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Autores principales: Schluter, Erik W., Mitz, Andrew R., Cheer, Joseph F., Averbeck, Bruno B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055617/
https://www.ncbi.nlm.nih.gov/pubmed/24921937
http://dx.doi.org/10.1371/journal.pone.0098692
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author Schluter, Erik W.
Mitz, Andrew R.
Cheer, Joseph F.
Averbeck, Bruno B.
author_facet Schluter, Erik W.
Mitz, Andrew R.
Cheer, Joseph F.
Averbeck, Bruno B.
author_sort Schluter, Erik W.
collection PubMed
description Fast-scan cyclic voltammetry (FSCV) is often used to measure real-time dopamine (DA) concentrations in awake, behaving rodents. Extending this technique to work in monkeys would provide a platform for advanced behavioral studies and a primate model for preclinical research. The present study demonstrates the feasibility of DA recordings in two awake monkeys (Macaca mulatta) using a mixture of techniques adapted from rodent, primate and brain slice work. We developed a long carbon fiber electrode to operate in the larger primate brain. This electrode was lowered into the striatum each day using a recording chamber and a detachable micromanipulator system. A manipulator also moved one or more tungsten stimulating electrodes into either the nearby striatum or the ventral tegmental area/substantia nigra pars compacta (VTA/SNc). We developed an electrical stimulation controller to reduce artifacts during electrical stimulation. We also introduce a stimulation-based methodology for estimating distances between electrodes in the brain. Dopamine responses within the striatum were evoked by either stimulation of the striatum near the FSCV electrode, or stimulation within the VTA/SNc. Unexpected juice rewards also evoked dopamine responses in the ventral striatum. Thus, we demonstrate that robust dopamine responses can be recorded from awake, behaving primates with FSCV. In addition, we describe how a stimulation technique borrowed from the neuroprosthetics field can activate the distributed monkey midbrain dopamine system in a way that mimics rodent VTA stimulation.
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spelling pubmed-40556172014-06-18 Real-Time Dopamine Measurement in Awake Monkeys Schluter, Erik W. Mitz, Andrew R. Cheer, Joseph F. Averbeck, Bruno B. PLoS One Research Article Fast-scan cyclic voltammetry (FSCV) is often used to measure real-time dopamine (DA) concentrations in awake, behaving rodents. Extending this technique to work in monkeys would provide a platform for advanced behavioral studies and a primate model for preclinical research. The present study demonstrates the feasibility of DA recordings in two awake monkeys (Macaca mulatta) using a mixture of techniques adapted from rodent, primate and brain slice work. We developed a long carbon fiber electrode to operate in the larger primate brain. This electrode was lowered into the striatum each day using a recording chamber and a detachable micromanipulator system. A manipulator also moved one or more tungsten stimulating electrodes into either the nearby striatum or the ventral tegmental area/substantia nigra pars compacta (VTA/SNc). We developed an electrical stimulation controller to reduce artifacts during electrical stimulation. We also introduce a stimulation-based methodology for estimating distances between electrodes in the brain. Dopamine responses within the striatum were evoked by either stimulation of the striatum near the FSCV electrode, or stimulation within the VTA/SNc. Unexpected juice rewards also evoked dopamine responses in the ventral striatum. Thus, we demonstrate that robust dopamine responses can be recorded from awake, behaving primates with FSCV. In addition, we describe how a stimulation technique borrowed from the neuroprosthetics field can activate the distributed monkey midbrain dopamine system in a way that mimics rodent VTA stimulation. Public Library of Science 2014-06-12 /pmc/articles/PMC4055617/ /pubmed/24921937 http://dx.doi.org/10.1371/journal.pone.0098692 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Schluter, Erik W.
Mitz, Andrew R.
Cheer, Joseph F.
Averbeck, Bruno B.
Real-Time Dopamine Measurement in Awake Monkeys
title Real-Time Dopamine Measurement in Awake Monkeys
title_full Real-Time Dopamine Measurement in Awake Monkeys
title_fullStr Real-Time Dopamine Measurement in Awake Monkeys
title_full_unstemmed Real-Time Dopamine Measurement in Awake Monkeys
title_short Real-Time Dopamine Measurement in Awake Monkeys
title_sort real-time dopamine measurement in awake monkeys
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055617/
https://www.ncbi.nlm.nih.gov/pubmed/24921937
http://dx.doi.org/10.1371/journal.pone.0098692
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