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Real-Time Dopamine Measurement in Awake Monkeys
Fast-scan cyclic voltammetry (FSCV) is often used to measure real-time dopamine (DA) concentrations in awake, behaving rodents. Extending this technique to work in monkeys would provide a platform for advanced behavioral studies and a primate model for preclinical research. The present study demonst...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055617/ https://www.ncbi.nlm.nih.gov/pubmed/24921937 http://dx.doi.org/10.1371/journal.pone.0098692 |
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author | Schluter, Erik W. Mitz, Andrew R. Cheer, Joseph F. Averbeck, Bruno B. |
author_facet | Schluter, Erik W. Mitz, Andrew R. Cheer, Joseph F. Averbeck, Bruno B. |
author_sort | Schluter, Erik W. |
collection | PubMed |
description | Fast-scan cyclic voltammetry (FSCV) is often used to measure real-time dopamine (DA) concentrations in awake, behaving rodents. Extending this technique to work in monkeys would provide a platform for advanced behavioral studies and a primate model for preclinical research. The present study demonstrates the feasibility of DA recordings in two awake monkeys (Macaca mulatta) using a mixture of techniques adapted from rodent, primate and brain slice work. We developed a long carbon fiber electrode to operate in the larger primate brain. This electrode was lowered into the striatum each day using a recording chamber and a detachable micromanipulator system. A manipulator also moved one or more tungsten stimulating electrodes into either the nearby striatum or the ventral tegmental area/substantia nigra pars compacta (VTA/SNc). We developed an electrical stimulation controller to reduce artifacts during electrical stimulation. We also introduce a stimulation-based methodology for estimating distances between electrodes in the brain. Dopamine responses within the striatum were evoked by either stimulation of the striatum near the FSCV electrode, or stimulation within the VTA/SNc. Unexpected juice rewards also evoked dopamine responses in the ventral striatum. Thus, we demonstrate that robust dopamine responses can be recorded from awake, behaving primates with FSCV. In addition, we describe how a stimulation technique borrowed from the neuroprosthetics field can activate the distributed monkey midbrain dopamine system in a way that mimics rodent VTA stimulation. |
format | Online Article Text |
id | pubmed-4055617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40556172014-06-18 Real-Time Dopamine Measurement in Awake Monkeys Schluter, Erik W. Mitz, Andrew R. Cheer, Joseph F. Averbeck, Bruno B. PLoS One Research Article Fast-scan cyclic voltammetry (FSCV) is often used to measure real-time dopamine (DA) concentrations in awake, behaving rodents. Extending this technique to work in monkeys would provide a platform for advanced behavioral studies and a primate model for preclinical research. The present study demonstrates the feasibility of DA recordings in two awake monkeys (Macaca mulatta) using a mixture of techniques adapted from rodent, primate and brain slice work. We developed a long carbon fiber electrode to operate in the larger primate brain. This electrode was lowered into the striatum each day using a recording chamber and a detachable micromanipulator system. A manipulator also moved one or more tungsten stimulating electrodes into either the nearby striatum or the ventral tegmental area/substantia nigra pars compacta (VTA/SNc). We developed an electrical stimulation controller to reduce artifacts during electrical stimulation. We also introduce a stimulation-based methodology for estimating distances between electrodes in the brain. Dopamine responses within the striatum were evoked by either stimulation of the striatum near the FSCV electrode, or stimulation within the VTA/SNc. Unexpected juice rewards also evoked dopamine responses in the ventral striatum. Thus, we demonstrate that robust dopamine responses can be recorded from awake, behaving primates with FSCV. In addition, we describe how a stimulation technique borrowed from the neuroprosthetics field can activate the distributed monkey midbrain dopamine system in a way that mimics rodent VTA stimulation. Public Library of Science 2014-06-12 /pmc/articles/PMC4055617/ /pubmed/24921937 http://dx.doi.org/10.1371/journal.pone.0098692 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Schluter, Erik W. Mitz, Andrew R. Cheer, Joseph F. Averbeck, Bruno B. Real-Time Dopamine Measurement in Awake Monkeys |
title | Real-Time Dopamine Measurement in Awake Monkeys |
title_full | Real-Time Dopamine Measurement in Awake Monkeys |
title_fullStr | Real-Time Dopamine Measurement in Awake Monkeys |
title_full_unstemmed | Real-Time Dopamine Measurement in Awake Monkeys |
title_short | Real-Time Dopamine Measurement in Awake Monkeys |
title_sort | real-time dopamine measurement in awake monkeys |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055617/ https://www.ncbi.nlm.nih.gov/pubmed/24921937 http://dx.doi.org/10.1371/journal.pone.0098692 |
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