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Small Interfering RNA Inhibition of Andes Virus Replication
Andes virus (ANDV) is the most common causative agent of hantavirus pulmonary syndrome (HPS) in the Americas, and is the only hantavirus associated with human-to-human transmission. Case fatality rates of ANDV-induced HPS are approximately 40%. There are currently no effective vaccines or antivirals...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055710/ https://www.ncbi.nlm.nih.gov/pubmed/24924189 http://dx.doi.org/10.1371/journal.pone.0099764 |
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author | Chiang, Cheng-Feng Albariňo, Cesar G. Lo, Michael K. Spiropoulou, Christina F. |
author_facet | Chiang, Cheng-Feng Albariňo, Cesar G. Lo, Michael K. Spiropoulou, Christina F. |
author_sort | Chiang, Cheng-Feng |
collection | PubMed |
description | Andes virus (ANDV) is the most common causative agent of hantavirus pulmonary syndrome (HPS) in the Americas, and is the only hantavirus associated with human-to-human transmission. Case fatality rates of ANDV-induced HPS are approximately 40%. There are currently no effective vaccines or antivirals against ANDV. Since HPS severity correlates with viral load, we tested small interfering RNA (siRNA) directed against ANDV genes as a potential antiviral strategy. We designed pools of 4 siRNAs targeting each of the ANDV genome segments (S, M, and L), and tested their efficacy in reducing viral replication in vitro. The siRNA pool targeting the S segment reduced viral transcription and replication in Vero-E6 cells more efficiently than those targeting the M and L segments. In contrast, siRNAs targeting the S, M, or L segment were similar in their ability to reduce viral replication in human lung microvascular endothelial cells. Importantly, these siRNAs inhibit ANDV replication even if given after infection. Taken together, our findings indicate that siRNAs targeting the ANDV genome efficiently inhibit ANDV replication, and show promise as a strategy for developing therapeutics against ANDV infection. |
format | Online Article Text |
id | pubmed-4055710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40557102014-06-18 Small Interfering RNA Inhibition of Andes Virus Replication Chiang, Cheng-Feng Albariňo, Cesar G. Lo, Michael K. Spiropoulou, Christina F. PLoS One Research Article Andes virus (ANDV) is the most common causative agent of hantavirus pulmonary syndrome (HPS) in the Americas, and is the only hantavirus associated with human-to-human transmission. Case fatality rates of ANDV-induced HPS are approximately 40%. There are currently no effective vaccines or antivirals against ANDV. Since HPS severity correlates with viral load, we tested small interfering RNA (siRNA) directed against ANDV genes as a potential antiviral strategy. We designed pools of 4 siRNAs targeting each of the ANDV genome segments (S, M, and L), and tested their efficacy in reducing viral replication in vitro. The siRNA pool targeting the S segment reduced viral transcription and replication in Vero-E6 cells more efficiently than those targeting the M and L segments. In contrast, siRNAs targeting the S, M, or L segment were similar in their ability to reduce viral replication in human lung microvascular endothelial cells. Importantly, these siRNAs inhibit ANDV replication even if given after infection. Taken together, our findings indicate that siRNAs targeting the ANDV genome efficiently inhibit ANDV replication, and show promise as a strategy for developing therapeutics against ANDV infection. Public Library of Science 2014-06-12 /pmc/articles/PMC4055710/ /pubmed/24924189 http://dx.doi.org/10.1371/journal.pone.0099764 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Chiang, Cheng-Feng Albariňo, Cesar G. Lo, Michael K. Spiropoulou, Christina F. Small Interfering RNA Inhibition of Andes Virus Replication |
title | Small Interfering RNA Inhibition of Andes Virus Replication |
title_full | Small Interfering RNA Inhibition of Andes Virus Replication |
title_fullStr | Small Interfering RNA Inhibition of Andes Virus Replication |
title_full_unstemmed | Small Interfering RNA Inhibition of Andes Virus Replication |
title_short | Small Interfering RNA Inhibition of Andes Virus Replication |
title_sort | small interfering rna inhibition of andes virus replication |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055710/ https://www.ncbi.nlm.nih.gov/pubmed/24924189 http://dx.doi.org/10.1371/journal.pone.0099764 |
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