Cargando…

Morphological and Proteomic Analysis Reveal the Role of Pistil under Pollination in Liriodendron chinense (Hemsl.) Sarg

Pollination is an important physiological process during which interaction between pollen and pistil occurs. This interaction could determine whether or not fertilization will occur and hence the ratio of plant seed setting. Liriodendron chinense (Hemsl.) Sarg. (L. chinense) exhibits a distinct phen...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Ming, Wang, Kun, Wang, Xin, Yang, Pingfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055720/
https://www.ncbi.nlm.nih.gov/pubmed/24924488
http://dx.doi.org/10.1371/journal.pone.0099970
_version_ 1782320712784019456
author Li, Ming
Wang, Kun
Wang, Xin
Yang, Pingfang
author_facet Li, Ming
Wang, Kun
Wang, Xin
Yang, Pingfang
author_sort Li, Ming
collection PubMed
description Pollination is an important physiological process during which interaction between pollen and pistil occurs. This interaction could determine whether or not fertilization will occur and hence the ratio of plant seed setting. Liriodendron chinense (Hemsl.) Sarg. (L. chinense) exhibits a distinct phenomenon where seed setting ratio is not more than 10% in natural environment. To explore the origin of this phenomenon, we conducted a comparative morphological and proteomic analysis on L. chinense pistils upon pollination. The morphological analysis showed that pollen grows well in vitro, but much slower on pistil or nutrient medium containing pistil extract. Proteomic analysis showed that 493 proteins had changed the expression after pollination. Among them, 468 and 51 proteins were identified by isobaric tags for relative and absolute quantitation and two-dimensional gel electrophoresis respectively, and 26 proteins were common in the two methods. After proteins functional categorization, 66 differentially expressed proteins that are involved in reproduction process were found. Further analysis showed that among the reproductive process related proteins, protein disulfide-isomerase A6 and four embryo-defective proteins showed closer relations with the low seed setting phenomenon. The results indicated that the element from pistil might be the main reason leading to low seed setting in L. chinense, which will provide new insights in the mechanisms underlying L. chinense reproduction process.
format Online
Article
Text
id pubmed-4055720
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-40557202014-06-18 Morphological and Proteomic Analysis Reveal the Role of Pistil under Pollination in Liriodendron chinense (Hemsl.) Sarg Li, Ming Wang, Kun Wang, Xin Yang, Pingfang PLoS One Research Article Pollination is an important physiological process during which interaction between pollen and pistil occurs. This interaction could determine whether or not fertilization will occur and hence the ratio of plant seed setting. Liriodendron chinense (Hemsl.) Sarg. (L. chinense) exhibits a distinct phenomenon where seed setting ratio is not more than 10% in natural environment. To explore the origin of this phenomenon, we conducted a comparative morphological and proteomic analysis on L. chinense pistils upon pollination. The morphological analysis showed that pollen grows well in vitro, but much slower on pistil or nutrient medium containing pistil extract. Proteomic analysis showed that 493 proteins had changed the expression after pollination. Among them, 468 and 51 proteins were identified by isobaric tags for relative and absolute quantitation and two-dimensional gel electrophoresis respectively, and 26 proteins were common in the two methods. After proteins functional categorization, 66 differentially expressed proteins that are involved in reproduction process were found. Further analysis showed that among the reproductive process related proteins, protein disulfide-isomerase A6 and four embryo-defective proteins showed closer relations with the low seed setting phenomenon. The results indicated that the element from pistil might be the main reason leading to low seed setting in L. chinense, which will provide new insights in the mechanisms underlying L. chinense reproduction process. Public Library of Science 2014-06-12 /pmc/articles/PMC4055720/ /pubmed/24924488 http://dx.doi.org/10.1371/journal.pone.0099970 Text en © 2014 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Ming
Wang, Kun
Wang, Xin
Yang, Pingfang
Morphological and Proteomic Analysis Reveal the Role of Pistil under Pollination in Liriodendron chinense (Hemsl.) Sarg
title Morphological and Proteomic Analysis Reveal the Role of Pistil under Pollination in Liriodendron chinense (Hemsl.) Sarg
title_full Morphological and Proteomic Analysis Reveal the Role of Pistil under Pollination in Liriodendron chinense (Hemsl.) Sarg
title_fullStr Morphological and Proteomic Analysis Reveal the Role of Pistil under Pollination in Liriodendron chinense (Hemsl.) Sarg
title_full_unstemmed Morphological and Proteomic Analysis Reveal the Role of Pistil under Pollination in Liriodendron chinense (Hemsl.) Sarg
title_short Morphological and Proteomic Analysis Reveal the Role of Pistil under Pollination in Liriodendron chinense (Hemsl.) Sarg
title_sort morphological and proteomic analysis reveal the role of pistil under pollination in liriodendron chinense (hemsl.) sarg
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055720/
https://www.ncbi.nlm.nih.gov/pubmed/24924488
http://dx.doi.org/10.1371/journal.pone.0099970
work_keys_str_mv AT liming morphologicalandproteomicanalysisrevealtheroleofpistilunderpollinationinliriodendronchinensehemslsarg
AT wangkun morphologicalandproteomicanalysisrevealtheroleofpistilunderpollinationinliriodendronchinensehemslsarg
AT wangxin morphologicalandproteomicanalysisrevealtheroleofpistilunderpollinationinliriodendronchinensehemslsarg
AT yangpingfang morphologicalandproteomicanalysisrevealtheroleofpistilunderpollinationinliriodendronchinensehemslsarg